Isolation, identification of a Cd-resistant strain N-17 and its potential in enhancing cadmium accumulation by Brassica juncea

doi:10.3969/j.issn.1004-1524.2017.05.18

›› 2017, Vol. 29 ›› Issue (5): 812-817.DOI: 10.3969/j.issn.1004-1524.2017.05.18

• Environmental Science • Previous Articles Next Articles

Isolation, identification of a Cd-resistant strain N-17 and its potential in enhancing cadmium accumulation by Brassica juncea

DU Dongxia, DU Yu, XU Jun, YIN Hongmei, HE Yuelin^*

Hunan Academy of Microbiology, Changsha 410009, China

Received:2017-01-09 Online:2017-05-20 Published:2017-06-06

Abstract

Abstract: A Cd-resistant strain N-17, with siderophore-producing, nitrogen-fixing and CdCO₃-solubilizing capacity, was isolated from paddy soil with Cd pollution. Combined with the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, N-17 was identified as Burkholderia sp. A pot experiment was done to investigate the effect of N-17 on phytoremediation of Cd polluted soil by Brassica juncea. It was shown that this strain could significantly increase the content of exchangeable cadmium in contaminated soil. Compared with control, the biomass and plant height of Brassica juncea inoculated with N-17 was increased by 9.41% and 20.2%, respectively. The cadmium content in aboveground and root of Brassica juncea inoculated with N-17 was increased by 49% and 67%, respectively.

Key words: siderophore, nitrogen-fixing capacity, Burkholderia sp., phytoremediation

CLC Number:

X172

DU Dongxia, DU Yu, XU Jun, YIN Hongmei, HE Yuelin. Isolation, identification of a Cd-resistant strain N-17 and its potential in enhancing cadmium accumulation by Brassica juncea[J]. , 2017, 29(5): 812-817.

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Isolation, identification of a Cd-resistant strain N-17 and its potential in enhancing cadmium accumulation by Brassica juncea

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