Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae

doi:10.3969/j.issn.1004-1524.2017.05.20

›› 2017, Vol. 29 ›› Issue (5): 824-830.DOI: 10.3969/j.issn.1004-1524.2017.05.20

• Food Science • Previous Articles Next Articles

Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae

YE Riying¹, WANG Yaling^{1, *}, HOU Hongwei¹, ZHANG Chunhui², SUN Lijun¹, XU Defeng¹

1. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. ;
2. Maindata Inspection and Certification Technology Co. Ltd., Qinzhou 535000, China

Received:2016-12-15 Online:2017-05-20 Published:2017-06-06

Abstract

Abstract: In order to detect nucleoside, cordycepic acid and amino acid contents in insect body and stroma of Cordyceps gryllotalpae, ultrasound technology was used in extracting water-soluble constituents of C. gryllotalpae, and HPLC detection system was established and used in detecting nucleoside, cordycepic acid and amino acid were detected respectively by spectrophotometer and automatic amino acid analyzer. The result showed that recovery of nucleoside detection was 95.73%-99.00%, the accuracy of appearance time was 0.05%-0.14%, the accuracy of peak area was 0.12%-0.84%, contents of uridine, inosine, vernine and adenosine of insect body and stroma were respectively 2.86, 0.43, 2.12, 0.17 and 3.53, 0.89, 3.51, 1.39 mg·g^-1. Recovery of cordycepic acid detection was 98.29%, cordycepic acid content in insect body and stroma were 109.64 and 127.46 mg·g^-1, and amino acid score of them were 56.3 and 62.8,essential amino acid index of them were 81.9 and 115.4. The result indicated that contents of nucleoside, cordycepic acid and nutritive value of protein in stroma were all more tnan those in insect body.

Key words: Cordyceps gryllotalpae, nucleoside, cordycepic acid, amino acid

CLC Number:

S567.35

YE Riying, WANG Yaling, HOU Hongwei, ZHANG Chunhui, SUN Lijun, XU Defeng. Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae[J]. , 2017, 29(5): 824-830.

References

[1] 李琴, 张驰. 不同原料来源的虫草素的提取研究概况[J]. 湖北民族学院学报(自然科学版), 2013, 31(2): 149-152.
LI Q, ZHANG C. Research profile on extracting cordycepin from different raw materials[J]. Journal of Hubei University for Nationalities ( Natural Science Edition ), 2013, 31(2): 149-152. (in Chinese with English abstract)
[2] 宋江峰, 刘春泉, 李大婧, 等. HPLC-DAD测定人工北冬虫夏草中虫草素含量[J]. 食品科学, 2008, 29(4): 352-354.
SONG J F, LIU C Q, LI D J, et al. Determination of cordycepin from cultured Cordyceps sinensis by HPLC-DAD[J]. Food Science , 2008, 29(4): 352-354. (in Chinese with English abstract)
[3] 彭日煌, 谢明勇, 王远兴, 等.RP-HPLC测定虫草菌粉中核苷类成分[J].食品与机械, 2005, 21(1): 61-63.
PENG R H, XIE M Y, WANG Y X, et al. Determination of nucleotides in cordyceps by RP-HPLC[J]. Food and Machinery , 2005, 21(1): 61-63.(in Chinese with English abstract)
[4] 汪宇, 于荣敏, 杨光照, 等. 高效液相色谱法测定蛹虫草人工固体培养物中核苷类化合物的含量[J]. 中国生化药物杂志, 2004, 25(5): 306-308.
WANG Y, YU R M, YANG G Z, et al. Determination of nucleosines in Cordyceps militaris by HPLC[J] . Chinese Journal of Biochemical Pharmaceutics , 2004, 25(5): 306-308. (in Chinese with English abstract)
[5] 王英娟, 李多伟, 王义潮, 等. 蛹虫草中虫草素、虫草多糖综合提取工艺研究[J]. 西北植物学报, 2005, 25(9): 1863-1867.
WANG Y J, LI D W, WANG Y C, et al. Integrated extracting technology of cordycepin and polysaccharides in Cordyceps militaris [J]. Acta Botanica Boreali-Occidentalia Sinica , 2005, 25(9): 1863-1867.(in Chinese with English abstract)
[6] 卢钢, 孙立伟, 高晓旭, 等. 北冬虫夏草中虫草素的微波辅助提取[J].东北林业大学学报, 2010, 38(7): 113-115.
LU G, SUN L W, GAO X X, et al. Microwave-assisted extraction of cordycepin from Cordyceps militaris [J]. Journal of Northeast Forestry University , 2010, 38(7): 113-115. (in Chinese with English abstract)
[7] 陈安徽, 陈宏伟, 徐洋,等. 蝉花虫草中核苷类成分的分离纯化和鉴定[J]. 食品科学, 2013, 34(1): 131-134.
CHEN A H, CHEN H W, XU Y, et al. Purification and identification of nucleoside components in Cordyceps cicadae fruit bodies[J]. Food Science , 2013, 34(1): 131-134. (in Chinese with English abstract)
[8] 凌建亚, 孙迎节, 吕鹏, 等. 虫草属真菌中虫草素的超声波提取及其毛细管电泳测定[J]. 菌物学报, 2002, 21(3): 394-399.
LING J Y, SUN Y J, LYU P, et al. Capillary zone electrophoresis determination of cordycepin in Cordyceps spp. extracted by using ultrasonic[J]. Mycosystema , 2002, 21(3): 394-399. (in Chinese with English abstract)
[9] 黄兰芳, 郭方遒, 梁逸曾, 等. HPLC-ESI-MS测定冬虫夏草和蚕蛹虫草中腺苷和虫草素含量[J]. 中国中药杂志, 2004, 29(8): 762-764.
HUANG L F, GUO F Q, LIANG Y Z, et al. Determination of adenosine and cordycepin in Cordyceps sinensis and C. militaris with HPLC-ESI-MS[J]. China Journal of Chinese Materia Medica , 2004, 29(8): 762-764.(in Chinese with English abstract)
[10] 刘丽, 孙玉飞, 冯菲, 等. RP-HPLC定量检测蛹虫草培养基中活性成分[J]. 河南科学, 2014, 32(8): 1436-1439.
LIU L, SUN Y F, FENG F, et al. Determination of bioactive components in culture medium of Cordyceps militaris by reversed-phase high performance liquid chromatography[J]. Henan Science , 2014, 32(8): 1436-1439. (in Chinese with English abstract)
[11] 孙忠华, 肖建辉, 迟强, 等. 高效液相色谱法测定三种虫草发酵液与菌丝体核苷化合物含量[J]. 天然产物研究与开发, 2014, 26(1): 2000-2003, 2061.
SUN Z H, XIAO J H, CHI Q, et al. Determination of nucleosides in fermentation broth and mycelium of three Cordyceps by high-performance liquid chromatograph[J]. Natural Product Research and Development , 2014, 26(1): 2000-2003, 2061. (in Chinese with English abstract)
[12] 雷宁, 杜树山, 倪雪梅, 等. RP-HPLC测定天然虫草与人工虫草中核苷类成分的含量[J]. 中国药学杂志, 2006, 41(12): 948-951.
LEI N, DU S S, NI X M, et al. Determination of nucleosides in natural Cordyceps Sinensis and cultured Cordyceps by RP-HPLC[J]. Chinese Pharmaceutical Journal , 2006, 41(12): 948-951.(in Chinese with English abstract)
[13] 周苏.冬虫夏草及虫草制品中活性成分检测方法的建立及含量测定[D].上海: 上海海洋大学, 2013.
ZHOU S. Establishment of detection method and determination of contents of active components in Cordyceps and products [D]. Shanghai: Shanghai Ocean University, 2013. (in Chinese with English abstract)
[14] 蔡友华, 范文霞, 刘学铭, 等. 超声-微波协同萃取巴西虫草菌丝体中甘露醇的研究[J]. 江西农业大学学报, 2008, 30(2): 348-353.
CAI Y H, FAN W X, LIU X M, et al. A study on ultrasonic-microwave synergistic extraction of mannitol in the mycelia in Cordyceps brasiliensis [J]. Acta Agriculturae Universitis Jiangxiensis , 2008, 30(2): 348-353.(in Chinese with English abstract)

Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 12

Recommended Articles

Metrics

Comments

[1]	ZHAO Guangmin, LIU Xiuting, DAI Bing, YANG Hua, LYU Wentao, WANG Yuanxia, XIAO Yingping. Effects of early weaning and dietary glutamine addition on serum free amino acids and their metabolites in piglets [J]. , 2020, 32(10): 1764-1771.
[2]	ZHANG Haojie, LIU Mei, LI Chunyan, HE Ran, LAN Jingchao, LUO Li, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic expression and immunofluorescence localization of nucleoside diphosphate kinase gene from Dirofilaria immitis [J]. , 2019, 31(9): 1453-1460.
[3]	MA Jie, ZHENG Hao, ZHOU Ping, CHEN Chunyan, WU Rui, MA Wei, SONG Lei, SUN Bo. Cloning and sequence analysis of StBSKs gene of Solanum tuberosum [J]. , 2019, 31(8): 1224-1230.
[4]	LIU Yongtao, DONG Jing, XIA Jingjin, CAO Cuiyu, XU Ning, YANG Qiuhong, AI Xiaohui. Effect and evaluation of different feed on texture properties and nutritional quality of Procambarus clarkia cultured in rice fields [J]. , 2019, 31(12): 1996-2004.
[5]	MA Linlong, JIN Xiaofang, CAO Dan, LIU Yanli, GONG Ziming, WANG Shengpeng, LIU Panpan. Suitability for processing green tea from high amino acid content of tea introduced to Hubei Province [J]. , 2017, 29(2): 251-260.
[6]	HUANG Shulun, ZHENG Kaiwen, HU Yang, LI Zhangcheng, CHENG Fangjun, SONG Zhenhui, ZHOU Zuoyong, LIU Guolin, LIN Chunfa, LIN Yongrun, ZHANG Tingting. Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes [J]. , 2017, 29(12): 1994-1999.
[7]	ZHANG Qiang1，2WU Caie1，*. Physicochemical properties and antioxidant activities of protein from Morchella esculenta mycelium [J]. , 2016, 28(8): 1408-.
[8]	LI Chun， CHU Wei\\|wei， LIU Huan， FEI Wen\\|chao， HAN Miao\\|miao. Numerical characterization of DNA sequences based on multisets of pseudo amino acids and its applications#br# [J]. , 2015, 27(7): 1244-.
[9]	XIAO Ming\\|li;YANG Qing;LIN Rui\\|feng;PU Yuan\\|zhu;*;BAO Xiu\\|ping;LI Xian. Improvement of tobacco quality by flavored proteinase degradation [J]. , 2014, 26(1): 0-185.
[10]	YANG Yong;LIU Qiang;;SONG Haixing;GUAN Chunyun;;RONG Xiangmin;PENG Jianwei. Effects of different NPK ratios on nutrients absorption,carbon and nitrogen metabolism, and grain yield of rapeseed YANG Yong1, LIU Qiang1,, SONG Haixing1, GUAN Chunyun2,, RONG Xiangmin1, PENG Jianwei1 (1Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resource Use, Hunan Provincial Key Laboratory of Plant Nutrition in Common University, College of Resource and Environment, Hunan Agriculture University, Changsha 410128,China; 2 National Center of Oilseed Crops Improvement,Hunan Branch,Changsha 410128,China) Abstract: A field experiment was conducted to investigate the effects of different NPK ratios on nutrients absorption, carbon and nitrogen metabolism, and grain yield of Xiangzayou 763 rapeseed. The results showed that the total amounts of free amino acids as well as the contents and total soluble sugar increased with increasing N levels,but there was no consistent effect for P and K levels on them. NPK accumulation and grain yields of rapeseed were increased with increasing N and P levels, but the influence of K was not obvious. Fertilizer use efficiencies of N and K decreased with increasing N and P levels. However, P fertilizer use efficiency increased first and then decreased with increasing P levels. The highest grain yield (2 231 kg·hm-2) was obtained from the treatment E (N∶P2O5∶K2O=10∶05∶05), followed by the treatment K (N∶P2O5∶K2O=1∶043∶057, which was 2 065 kg·hm-2. Effects of yield component factors on grain yields of rapeseed decreased in the order: effective pod numbers per plant>seeds per pod >1000-seeds weight． Key words: Effects of different NPK ratios on nutrients absorption,carbon and nitrogen metabolism, and grain yield of rapeseed [J]. , 2012, 24(1): 0-104.
[11]	JI Yu-mei;ZHAO Li-wei. Detection and analysis of free amino acids in main maize inbred lines by HPLC method [J]. , 2011, 23(4): 0-654.
[12]	CHEN Guo-lin;WU Jian-guo;SHI Chun-hai . Analysis of the genetic effects on the trait of seed amino acid content in crops [J]. , 2009, 21(05): 0-528.