Effect of application of amino acid fertilizer on spring maize cultivation under nitrogen reduction

doi:10.3969/j.issn.1004-1524.2022.04.01

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (4): 661-670.DOI: 10.3969/j.issn.1004-1524.2022.04.01

• Crop Science • Previous Articles Next Articles

Effect of application of amino acid fertilizer on spring maize cultivation under nitrogen reduction

HU Kaibo¹^,²(), YANG Qingxia¹, LI Yang¹, WU Kaixian³, ZHAO Ping¹^,²^,^*(), LONG Guangqiang¹^,²

1. College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China
2. Yunnan Scientific Observation Station for Cultivated Land Conservation of the Ministry of Agriculture and Rural Affairs, Kunming 650201, China
3. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China

Received:2021-04-22 Online:2022-04-25 Published:2022-04-28
Contact: ZHAO Ping

Abstract

Abstract:

In order to explore the effect of application of amino acid fertilizer on spring maize cultivation under nitrogen reduction, a field plot experiment was conducted with 6 treatments: N0, no nitrogen applied; U100, conventional N rate, 250 kg·hm^-2; U80, N reduction by 20% compared with U100; U60, N reduction by 40% compared with U100; A80, application of amino acid fertilization based on U80; A60, application of amino acid fertilization based on U60. The yield, and main physiological characteristics at tasseling period, nitrogen uptake and utilization of maize at maturity period under different treatments were determined. It was shown that compared with U100, N reduction rate of 20% (U80 and A80 treatments) did not significantly decrease maize yield, yet significantly (P<0.05) increased nitrogen use efficiency by 35.8%-38.8% and harvest index by 41.8%-57.2%. When N reduction rate was 40%, maize yield was significantly (P<0.05) decreased by 20.6% under U60 treatment as compared with U100, yet maize yield was not significantly changed under A60 treatment. Besides, the nitrogen use efficiency was significantly (P<0.05) increased by 30.0% under A60 treatment as compared with U100. However, A60 and U60 treatments had no significant effect on harvest index of maize as compared with U100. Under the same N reduction rate, the intracellular CO₂ concentration, chlorophyll content (SPAD value), photosynthetic nitrogen use efficiency, and activities of glutamic oxaloacetic transaminase and glutamate dehydrogenase under A80 treatment were significantly (P<0.05) elevated as compared with U80 treatment, and the transpiration rate and activities of glutamine synthetase and glutamate synthase were significantly (P<0.05) elevated under A60 treatment as compared with U60 treatment. Thus, it was concluded that N reduction rate of 20% would not decrease maize yield based on the conventional N rate under the experiment conditions. Under the same N rate, application of amino acid fertilizers would further increase nitrogen use efficiency.

Key words: amino acid, spring maize, photosynthetic characteristics, nitrogen use efficiency, nitrogen assimilation enzymes, yield

CLC Number:

S147.34

HU Kaibo, YANG Qingxia, LI Yang, WU Kaixian, ZHAO Ping, LONG Guangqiang. Effect of application of amino acid fertilizer on spring maize cultivation under nitrogen reduction[J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 661-670.

Figures/Tables 7

Table 1 Effects of different treatments on yield,aboveground biomass,harvest index and yield component

处理 Treatment	穗长 Ear length/ cm	秃尖长 Bare tip length/cm	穗行数 Kernel row number	行粒数 Kernel number per row	百粒重 100-grain weight/g	产量 Yield/(kg· hm^-2)	地上部生物量 Aboveground biomass/ (kg·hm^-2)	收获指数 Harvest index/%
U100	20.6±4.2 a	2.1±0.5 a	19±3 b	36±1 a	26.4±1.5 b	8 656±126.9 ab	29 164.8±2 951.0 bc	40.7±1.5 b
U80	18.9±1.5 ab	1.3±0.3 bc	22±1 a	39±1 a	26.3±0.7 b	9 503±1 001.3 a	35 574.1±1 335.7 a	64.0±3.6 a
A80	17.8±2.9 ab	0.8±0.3 c	23±2 a	37±2 a	29.0±2.3 a	9 163±1 313.3 a	32 052.0±1 125.5 ab	57.7±2.5 a
U60	14.5±3.6 b	1.8±0.4 ab	19±1 b	38±3 a	25.5±0.5 b	6 868±706.8 c	26 081.6±2 732.5 c	38.3±2.5 b
A60	16.3±0.4 ab	1.7±0.3 ab	20±1 ab	36±3 a	25.7±1.1 b	7 411±983.3 bc	28 048.0±1 625.2 c	34.0±8.2 b

Fig.1 Soil ammonium nitrogen and nitrate nitrogen content under different treatments at different growth periods Bars marked without the same letters indicated significant difference at P<0.05 at the same growth period.

Table 2 Effects of different treatments on nitrogen distribution and use efficiency of spring maize at harvest stage

处理 Treatment	籽粒Grain		秸秆Straw		根系root		氮吸收总量 Total N uptake/(kg· hm^-2)	NUE/%
处理 Treatment	吸氮量 N uptake/ (kg·hm^-2)	比例 Proportion/%	吸氮量 N uptake/ (kg·hm^-2)	比例 Proportion/%	吸氮量 N uptake/ (kg·hm^-2)	比例 Proportion/%	氮吸收总量 Total N uptake/(kg· hm^-2)	NUE/%
U100	90.3± 12.4 ab	73.8± 9.6 ab	20.4± 7.3 b	16.7± 6.0 ab	11.7± 2.7 ab	9.5± 1.5 ab	122.4± 9.1 bc	42.13± 5.70 b
U80	102.7± 16.9 ab	75.3± 8.7 ab	18.4± 5.9 b	13.5± 4.0 b	15.3± 6.7 a	11.2± 4.6 a	136.4± 18.2 ab	57.20± 2.35 a
A80	97.2± 18.0 ab	68.3± 6.6 b	29.7± 8.3 a	20.9± 6.3 a	15.4± 5.0 a	10.8± 5.1 a	142.3± 11.0 a	58.47± 3.55 a
U60	79.1± 6.8 b	78.0± 4.4 a	13.8± 2.9 c	13.6± 1.9 b	8.7± 3.1 ab	8.6± 2.9 ab	101.5± 8.8 c	50.72± 4.72 ab
A60	111.8± 3.5 a	79.2± 3.7 a	23.5± 4.2 ab	16.7± 1.9 ab	5.8± 3.3 b	4.1± 2.1 b	141.1± 9.5 a	54.74± 7.05 a

Table 3 Effects of different treatments on physiological indexes of maize leaf

处理 Treatment	P_n/(μmol· m^-2·s^-1)	C_i/(μmol· mol^-1)	T_r/(μmol· m^-2·s^-1)	G_s/(μmol· m^-2·s^-1)	SPAD	PNUE/(μmol· g^-1·s^-1)
U100	20.5±1.2 a	152.4±4.7 d	3.3±0.2 a	0.23±0.06 b	39.2±1.5 b	59.0±4.6 b
U80	20.0±0.4 a	133.5±5.5 e	3.2±0.1 a	0.27±0.03 ab	38.9±1.3 b	63.0±3.9 b
A80	17.3±0.5 b	205.6±1.4 c	3.1±0.2 a	0.32±0.03 a	41.8±1.0 a	69.3±6.8 a
U60	15.8±0.9 b	295.2±10.3 a	2.0±0.2 c	0.11±0.03 c	30.2±1.4 c	45.4±1.1 c
A60	17.3±0.6 b	230.5±1.6 b	2.3±0.1 b	0.16±0.04 c	32.2±1.6 c	54.8±11.5 bc

Table 4 Effects of different treatments on nitrogen assimilation enzymes activities

处理Treatment	GDH/(nmol·min^-1·g^-1)	GOT/(nmol·min^-1·g^-1)	GS/(U·g^-1)	GOGAT/(nmol·min^-1·g^-1)
U100	76.75±5.17 a	76.69±2.32 b	21.60±0.35 a	117.90±11.66 a
U80	35.16±2.68 c	74.06±5.11 b	21.03±1.40 a	105.51±7.44 ab
A80	57.29±1.24 b	86.86±3.99 a	14.29±1.05 b	110.49±13.90 ab
U60	39.62±8.16 c	62.54±1.93 c	15.58±2.18 b	57.34±5.24 c
A60	42.91±2.55 c	64.84±1.60 c	20.98±3.89 a	91.73±12.04 b

Table 5 Correlation within different indexes

指标 Index	氮吸收总量 Total N uptake	P_n	$NH 4 +$ -N	$NO 3 -$ -N	地上部全氮 Total N in shoot	SPAD	PNUE	GOT	GOGAT
产量Yield	0.532^*	0.537^*	0.123	0.721^**	-0.321	0.781^**	0.581^*	0.572^*	0.532^*
NUE	0.205	-0.243	0.717^**	0.334	-0.739^**	-0.204	0.126	0.153	-0.056

Table 5 Correlation within different indexes

指标 Index	氮吸收总量 Total N uptake	P_n	$NH 4 +$ -N	$NO 3 -$ -N	地上部全氮 Total N in shoot	SPAD	PNUE	GOT	GOGAT
产量Yield	0.532^*	0.537^*	0.123	0.721^**	-0.321	0.781^**	0.581^*	0.572^*	0.532^*
NUE	0.205	-0.243	0.717^**	0.334	-0.739^**	-0.204	0.126	0.153	-0.056

Fig.2 Redundancy analysis of spring maize yield and nitrogen use efficiency TNU, Total nitrogen uptake; Pn, Net photosynthetic rate; SPAD, Chlorophyll content(SPAD value); PNUE, Photosynthetic nitrogen utilization efficiency; GOGAT,Glutamate synthetase; GOT, Glutamic oxaloacetic transaminase; Yield, Yield of maize; NUE, Nitrogen use efficiency; TNS, Total nitrogen content in aboveground part (at tasseling period); NH 4 +-N, Soil ammonium nitrogen content (at tasseling period); NO 3 --N,Soil nitrate nitrogen content (at tasseling period).

References 28

[1]	巨晓棠, 谷保静. 我国农田氮肥施用现状、问题及趋势[J]. 植物营养与肥料学报, 2014, 20(4): 783-795.
	JU X T, GU B J. Status-quo, problem and trend of nitrogen fertilization in China[J]. Plant Nutrition and Fertilizer Science, 2014, 20(4): 783-795. (in Chinese with English abstract)
[2]	李焕春, 赵娜, 莎娜, 等. 滴灌条件下减量施肥对玉米产量及肥料利用率的影响[J]. 北方农业学报, 2017, 45(6): 39-43.
	LI H C, ZHAO N, SHA N, et al. Effects of reduced fertilization on yield and fertilizer use efficiency of maize under drip irrigation[J]. Inner Mongolia Agricultural Science and Technology, 2017, 45(6): 39-43. (in Chinese)
[3]	徐丽娜, 闫艳, 梅沛沛, 等. 基肥减氮对夏玉米品种产量形成和氮利用的影响[J]. 耕作与栽培, 2020, 40(2): 7-10.
	XU L N, YAN Y, MEI P P, et al. Effects of reducing nitrogen in base fertilizer on yield and nitrogen use efficiency of summer maize[J]. Tillage and Cultivation, 2020, 40(2): 7-10. (in Chinese with English abstract)
[4]	朱兆良, 金继运. 保障我国粮食安全的肥料问题[J]. 植物营养与肥料学报, 2013, 19(2): 259-273.
	ZHU Z L, JIN J Y. Fertilizer use and food security in China[J]. Plant Nutrition and Fertilizer Science, 2013, 19(2): 259-273. (in Chinese with English abstract)
[5]	WANG P, WANG Z K, SUN X C, et al. Interaction effect of nitrogen form and planting density on plant growth and nutrient uptake in maize seedlings[J]. Journal of Integrative Agriculture, 2019, 18(5): 1120-1129. DOI
[6]	ABBASI M K, TAHIR M M, RAHIM N. Effect of N fertilizer source and timing on yield and N use efficiency of rainfed maize (Zea mays L.) in Kashmir-Pakistan[J]. Geoderma, 2013, 195/196: 87-93. DOI URL
[7]	吕鹏, 张吉旺, 刘伟, 等. 施氮时期对超高产夏玉米产量及氮素吸收利用的影响[J]. 植物营养与肥料学报, 2011(5): 1099-1107.
	LÜ P, ZHANG J W, LIU W, et al. Effects of nitrogen application dates on yield and nitrogen use efficiency of summer maize in super-high yield conditions[J]. Plant Nutrition and Fertilizer Science, 2011(5): 1099-1107. (in Chinese with English abstract)
[8]	李广浩, 董树亭, 赵斌, 等. 不同土壤水分状况下实现夏玉米高产及氮素高效的控释尿素用量研究[J]. 植物营养与肥料学报, 2018, 24(3): 579-589.
	LI G H, DONG S T, ZHAO B, et al. Optimal application rates of controlled release urea for high yield and high nitrogen use efficiency of summer maize under different soil water conditions[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(3): 579-589. (in Chinese with English abstract)
[9]	狄雅莉. 化肥配施氨基酸肥料在冬小麦-夏玉米轮作系统中的减氮增效研究[D]. 沈阳: 沈阳农业大学, 2020.
	DI Y L. Study on nitrogen reducing and increasing effect of combined application of chemical fertilizer and amino acid fertilizer in winter wheat-summer maize rotation system[D]. Shenyang: Shenyang Agricultural University, 2020. (in Chinese with English abstract)
[10]	WANG H J, WU L H, WANG M Y, et al. Effects of amino acids replacing nitrate on growth, nitrate accumulation, and macroelement concentrations in pakchoi (Brassica chinensis L.)[J]. Pedosphere, 2007, 17(5): 595-600. DOI URL
[11]	袁伟, 董元华, 王辉. 植物氨基酸多元素肥料生物效应的研究进展[J]. 土壤, 2009, 41(1): 16-20.
	YUAN W, DONG Y H, WANG H. A review: biological effect of plant amino acids trace-element fertilizers[J]. Soils, 2009, 41(1): 16-20. (in Chinese with English abstract)
[12]	陈明昌, 程滨, 张强, 等. 土施L-蛋氨酸、L-苯基丙氨酸、L-色氨酸对玉米生长和养分吸收的影响[J]. 应用生态学报, 2005, 16(6): 1033-1037.
	CHEN M C, CHENG B, ZHANG Q, et al. Effects of applying L-methionine, L-phenylalanine and L-tryptophan on Zea mays growth and its nutrient uptake[J]. Chinese Journal of Applied Ecology, 2005, 16(6): 1033-1037. (in Chinese with English abstract)
[13]	王子宁. 氨基酸肥料与化肥配施对设施番茄产量与品质及氮素效应的研究[D]. 沈阳: 沈阳农业大学, 2018.
	WANG Z N. Amino acid fertilizers and fertilizers applied to facilities study on yield and quality and nitrogen effect of tomato[D]. Shenyang: Shenyang Agricultural University, 2018. (in Chinese with English abstract)
[14]	吴玉群, 史振声, 李荣华, 等. 植物氨基酸液肥对爆裂玉米产量及生理指标的影响[J]. 种子, 2006, 25(4): 73-75.
	WU Y Q, SHI Z S, LI R H, et al. Effects of plant amino acid liquid fertilizer on yield and physiological indexes of popcorn[J]. Seed, 2006, 25(4): 73-75. (in Chinese)
[15]	王兰天. 含氨基酸水溶肥料在玉米和白菜上的应用效果研究[J]. 河南科学, 2013, 31(7): 972-974.
	WANG L T. Application of water-soluble fertilizer containing amino acids on maize and Chinese cabbage[J]. Henan Science, 2013, 31(7): 972-974. (in Chinese with English abstract)
[16]	吴玉群, 史振声, 李荣华, 等. 植物氨基酸液肥对甜玉米产量及生理指标的影响[J]. 玉米科学, 2006, 14(5): 130-133.
	WU Y Q, SHI Z S, LI R H, et al. Effect of plant amino acid liquid fertilizer on the physiological index and yield of sweet corn[J]. Journal of Maize Sciences, 2006, 14(5): 130-133. (in Chinese with English abstract)
[17]	代丽萍, 史海滨, 苗庆丰, 等. 不同灌水量下除草剂对玉米生长与籽粒农药残留量的影响[J]. 灌溉排水学报, 2021, 40(5): 30-38.
	DAI L P, SHI H B, MIAO Q F, et al. Effects of herbicides on maize growth and grain pesticide residues under different irrigation amounts[J]. Journal of Irrigation and Drainage, 2021, 40(5): 30-38. (in Chinese with English abstract)
[18]	房娜娜, 卢宗云, 石元亮, 等. 肥料增效剂“保肥思”对春玉米苗期土壤有效氮的影响[J]. 辽宁农业科学, 2016(1): 32-35.
	FANG N N, LU Z Y, SHI Y L, et al. Effect of fertilizer synergist BIOSOFT on soil available nitrogen at seedling stage of spring maize[J]. Liaoning Agricultural Sciences, 2016(1): 32-35. (in Chinese with English abstract)
[19]	李超英, 计小江, 吴春艳. 施用不同新型增效尿素对水稻产量和氮肥利用率的影响[J]. 浙江农业学报, 2013, 25(2): 333-338.
	LI C Y, JI X J, WU C Y. Effects of application of different novel synergistic urea on the yield and nitrogen use efficiency of rice[J]. Acta Agriculturae Zhejiangensis, 2013, 25(2): 333-338. (in Chinese with English abstract)
[20]	赵士诚, 裴雪霞, 何萍, 等. 氮肥减量后移对土壤氮素供应和夏玉米氮素吸收利用的影响[J]. 植物营养与肥料学报, 2010, 16(2): 492-497.
	ZHAO S C, PEI X X, HE P, et al. Effects of reducing and postponing nitrogen application on soil N supply, plant N uptake and utilization of summer maize[J]. Plant Nutrition and Fertilizer Science, 2010, 16(2): 492-497. (in Chinese with English abstract)
[21]	张健, 李燕婷, 袁亮, 等. 氨基酸发酵尾液可促进樱桃番茄对水溶肥料氮素的吸收利用[J]. 植物营养与肥料学报, 2018, 24(1): 114-121.
	ZHANG J, LI Y T, YUAN L, et al. Tail liquid from amino acid fermentation could improve the uptake and utilization of water soluble fertilizer nitrogen by cherry tomato[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(1): 114-121. (in Chinese with English abstract)
[22]	李荣华, 史振声, 陈风玉, 等. 植物氨基酸液肥的增产作用及其机理研究[C]// 中国腐植酸工业协会.第七届全国绿色环保肥料(农药)新技术、新产品交流会论文集. 北京: 中国腐植酸工业协会, 2008:7.
[23]	孙永平, 张治平, 徐呈祥, 等. 5-氨基乙酰丙酸处理对低温下西瓜叶片快速叶绿素荧光诱导曲线的影响[J]. 园艺学报, 2009, 36(5): 671-678.
	SUN Y P, ZHANG Z P, XU C X, et al. Effect of ALA on fast chlorophyll fluorescence induction dynamics of watermelon leaves under chilling stress[J]. Acta Horticulturae Sinica, 2009, 36(5): 671-678. (in Chinese with English abstract)
[24]	张丽娟, 杨升辉, 杨恒山, 等. 高产栽培下氮肥运筹对春玉米光合特性的影响[J]. 安徽农业科学, 2012, 40(5): 2598-2601.
	ZHANG L J, YANG S H, YANG H S, et al. Effects of nitrogen fertilizer application schemes on spring maize(Zea mays L.) photosynthetic characteristics under hyper-yield cultivation[J]. Journal of Anhui Agricultural Sciences, 2012, 40(5): 2598-2601. (in Chinese with English abstract)
[25]	CALVO P, NELSON L, KLOEPPER J W. Agricultural uses of plant biostimulants[J]. Plant and Soil, 2014, 383(1/2): 3-41. DOI URL
[26]	陆景陵. 植物营养学:上册[M]. 2版. 北京: 中国农业大学出版社, 2003: 23-35.
[27]	吴良欢, 陶勤南. 水稻氨基酸态氮营养效应及其机理研究[J]. 土壤学报, 2000, 37(4): 464-473.
	WU L H, TAO Q N. Effects of amino acid-N on rice nitrogen nutrition and its mechanism[J]. Acta Pedologica Sinica, 2000, 37(4): 464-473. (in Chinese with English abstract)
[28]	田雁飞, 马友华, 褚进华, 等. 水稻减量化施肥与氨基酸水溶性肥配施效果研究[J]. 中国农学通报, 2011, 27(15): 34-39.
	TIAN Y F, MA Y H, CHU J H, et al. Research on effects of fertilizing decreasing with applying water-solubility amino acid fertilizer on rice[J]. Chinese Agricultural Science Bulletin, 2011, 27(15): 34-39. (in Chinese with English abstract)

Effect of application of amino acid fertilizer on spring maize cultivation under nitrogen reduction

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 28

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Jingshang, ZHANG Xiaojun, CHEN Shengchang, JIANG Jinhua, XIANG Yun, TU Pingguang, LOU Fangfang, YANG Hua, XIAO Yingping. Developmental changes of amino acid profiles in muscle and serum and their correlation with muscle growth of Jinhua pigs [J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 687-694.
[2]	WANG Huiru, LI Jianshe, YAN Sihua, GAO Yanming. Effect of prunning patterns on canopy light interception characteristics and chlorophyll fluorescence parameters in cherry tomato [J]. Acta Agriculturae Zhejiangensis, 2022, 34(3): 525-533.
[3]	YUAN Wenya, KANG Yichen, YANG Xinyu, ZHNAG Ruyan, ZHOU Chuntao, WANG Yong, CHEN Xipeng, YU Huifang, QIN Shuhao. Effects of rhizosphere soil extract of Qingshui alfalfa (Medicago sativa L.) on enzyme activities and microbial communities in rhizosphere soil of continuous cropping potato [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 240-247.
[4]	CUI Wenfang, CHEN Jing, LU Fukuan, QIN Li, QIN Dezhi, WANG Liping, GAO Julin. Effects of biochar application combined with nitrogen reduction on yield and nitrogen use efficiency of maize [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 248-254.
[5]	GAO Xin, YANG Hengshan, ZHANG Ruifu, ZHANG Yuqin, LI Rui, ZHANG Mingwei. Difference analysis on seed yield and root cap characteristics for spring maize under water fertilizer and high yield optimization of shallow burying drip irrigation [J]. Acta Agriculturae Zhejiangensis, 2022, 34(1): 1-9.
[6]	YANG Chao, LIU Minzhu, LI Qiang, HAN Tao, PENG Liangzhi, LING Lili, FU Xingzheng, CHUN Changpin, CAO Li, HE Yizhong. Effects of different light-emitting diode (LED) light quality on growth, development and photosynthetic characteristics of Jinqiu Shatangju seedlings [J]. Acta Agriculturae Zhejiangensis, 2022, 34(1): 89-97.
[7]	WANG Jia, MU Ruirui, YANG Qiaoqiao, LIU Wei, ZHANG Yuehe, KANG Jianhong. Effects of potassium application rate on chlorophyll fluorescence characteristics and yield of spring maize in Ningxia under integrated drip irrigation [J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1347-1357.
[8]	QI Zhenyu, CAI Licong, HU Weizhen, CAI Pan, ZHANG Longping, REN Yanyun, ZHOU Yanhong. Effects of reduced application of chemical fertilizers and ratio of base and topdressing fertilizers on yield and quality of garlic in integrated mode of water and fertilizer [J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1409-1415.
[9]	LIU Yanwei, ZHOU Xiao, YANG Qiliang, CHA Pinyuan. Effects of fertilization ratio and irrigation level on growth characteristics and incidence rate of Panax notoginseng [J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1426-1435.
[10]	WANG Feng, SHEN Jianghua, CHEN Ruoxia, SHI Jun, REN Shaopeng, JIN Shuquan, YAO Hongyan, ZHU Defeng, DAI Yaolu. Effects of reduced nitrogen application on yield and nitrogen agronomic efficiency of Yongyou indica-japonica hybrid rice [J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 984-992.
[11]	LI Qingbin, QIN Benben, LI Yingying, FAN Kaifeng, YANG Dong, CHEN Lei, LIU Kun. Effects of continuous rain and sunless weather on microclimate, strawberry growth and quality in greenhouse [J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 831-839.
[12]	TANG Hong, HUANG Tao, LIU Wei, HUANG Chengqian, HUANG Wentao, ZHENG Shuoli, CHEN Baibing. Comparative study on photosynthetic characteristics of 4 varieties of Malus sp. [J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 846-854.
[13]	ZHANG Ting, LIU Huiqin, GUO Qinwei, LI Chaosen, ZHANG Xinhui, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Principal component analysis and cluster analysis for evaluating free amino acids of 16 pepper materials [J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 640-650.
[14]	LI Baoxian, WANG Baojun, HUAI Yan, SHEN Yaqiang, ZHANG Hongmei, CHENG Wangda. Effects of integrated rice-redclaw crayfish farming system on soil nutrients, carbon pool and rice quality [J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 688-696.
[15]	ZHANG Hui, CHE Xusheng, LYU Jian, GOU Zhaohui, QIN Qijie, LUO Jian, ZHANG Guobin. Effects of irrigation amount and nitrogen forms on production and nitrogen metabolism of broccoli [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 308-315.