Effects of rhizosphere soil extract of Qingshui alfalfa (Medicago sativa L.) on enzyme activities and microbial communities in rhizosphere soil of continuous cropping potato

doi:10.3969/j.issn.1004-1524.2022.02.04

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (2): 240-247.DOI: 10.3969/j.issn.1004-1524.2022.02.04

• Crop Science • Previous Articles Next Articles

Effects of rhizosphere soil extract of Qingshui alfalfa (Medicago sativa L.) on enzyme activities and microbial communities in rhizosphere soil of continuous cropping potato

YUAN Wenya(), KANG Yichen, YANG Xinyu, ZHNAG Ruyan, ZHOU Chuntao, WANG Yong, CHEN Xipeng, YU Huifang, QIN Shuhao^*()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2021-05-31 Online:2022-02-25 Published:2022-03-02
Contact: QIN Shuhao

Abstract

Abstract:

In order to alleviate the continuous cropping obstacle of potato, a pot experiment was carried out with Xindaping potato as the test material. Five different ratios of 4-year-old Qingshui alfalfa (Medicago sativa L.)rhizosphere soil extracts and water mixed solution (1∶1, 2∶1, 3∶1, 4∶1, 5∶1) were set as treatments, and the control group(CK) was irrigated with the same amount of water. Effects of different concentrations of Qingshui alfalfa rhizosphere soil extracts on enzyme activities and microbial communities in continuous cropping potato rhizosphere soil environment was explored. The results showed that catalase and alkaline phosphatase activities in soil treated with the mixed solution (4∶1) were enhanced by 10.14% and 47.65% compared with CK. Compared with CK, number of anaerobic autogenic nitrogen-fixing bacteria and denitrifying bacteria increased by 54.80% and 36.78% and the potato yield was improved by 10.52%. Treated with mixed solution (3∶1), activities of urease and sucrase in soil were increased by 51.70% and 55.27% compared with CK. Compared with the CK, number of bacteria, actinomycetes and aerobic autogenic nitrogen-fixing bacteria were improved by 7.77%, 58.91% and 47.57%. And the number of fungi was significantly reduced, 12.42% lower than CK.Soil catalase activity of the mixed solution of 1∶1 was only lower than that of the mixed solution of 4∶1, and number of aerobic autogenic nitrogen-fixing bacteria was only lower than that of the mixed solution of 3∶1. Considering all these indicators, effect of treatment with mixed solution 2∶1 and 5∶1 were relatively poor. Different concentrations of Qingshui alfalfa soil extracts might increase enzyme activity and the number of some microorganisms, and reduce the number of fungi in potato rhizosphere soil environment. Thus, microbial community structure might be effectively ameliorated in the rhizosphere soil environment, thereby alleviating the continuous cropping obstacles of potato, and improving potato yield. Moreover, mixed solution with (4∶1) had the best effect.

Key words: potato, alfalfa, crop rotation, soil, microbial community, yield

CLC Number:

S532

YUAN Wenya, KANG Yichen, YANG Xinyu, ZHNAG Ruyan, ZHOU Chuntao, WANG Yong, CHEN Xipeng, YU Huifang, QIN Shuhao. Effects of rhizosphere soil extract of Qingshui alfalfa (Medicago sativa L.) on enzyme activities and microbial communities in rhizosphere soil of continuous cropping potato[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 240-247.

Figures/Tables 6

Table 1 Physio-chemical properties of tested soils

供试土壤 Experimental soil	速效氮 Available nitrogen/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	pH值 pH value	电导率 Conductivity/ (mS·m^-1)	阳离子交换量 Cation exchange capacity/ (cmol·kg^-1)
清水苜蓿根际土壤Qingshui alfalfa rhizosphere soil	9.78	15.11	41.44	7.92	147.43	204.14
马铃薯根际土壤Potato rhizosphere soil	11.78	13.71	142.42	7.90	186.13	211.02

Fig.1 Effects of Qingshui alfalfa soil extracts on potato rhizosphere soil enzyme activities CK,Control group (irrigated with the same amount of water); T1-T5, Qingshui alfalfa rhizosphere soil extracts and water mixed by 1∶1, 2∶1, 3∶1, 4∶1, 5∶1. Data marked without the same lowercase letters indicated significant differences at P<0.05. The same as below.

Table 2 Effects of Qingshui alfalfa soil extracts on the number of bacteria, fungi and actinomycetes in potato rhizosphere soil 105 CFU·g-1

处理 Treatment	细菌 Bacteria	真菌 Fungus	放线菌 Actinomycetes
CK	401.8±3.3 b	33.0±1.0 b	20.2±0.8 d
T1	406.9±26.2 ab	39.4 ±1.9 a	29.3±1.2 c
T2	395.6±9.6 ab	29.4±0.1 c	31.3±1.1 ab
T3	433.0±21.6 a	28.9±1.2 c	32.1±1.4 a
T4	426.7±11.8 ab	38.2±0.6 a	30.1±0.8 bc
T5	411.0±23.0 ab	40.2±1.0 a	29.6±0.7 bc

Fig.2 Effects of Qingshui alfalfa soil extracts on the number of voluntary nitrogen-fixing bacteria in potato rhizosphere soil

Fig.3 Effects of Qingshui alfalfa soil extracts on the number of nitrifying bacteria in potato rhizosphere soil

Fig.4 Effects of Qingshui alfalfa alfalfa soil extracts on potato yield

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Effects of rhizosphere soil extract of Qingshui alfalfa (Medicago sativa L.) on enzyme activities and microbial communities in rhizosphere soil of continuous cropping potato

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