Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress

doi:10.3969/j.issn.1004-1524.2017.07.12

›› 2017, Vol. 29 ›› Issue (7): 1144-1150.DOI: 10.3969/j.issn.1004-1524.2017.07.12

• Horticultural Science • Previous Articles Next Articles

Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress

GAO Fan¹, XIA Hui², YUAN Xuezhen¹, HUANG Shouyi¹, LIU Ji³, LIANG Dong^{2, *}

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China;
3. Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China

Received:2017-03-03 Online:2017-07-20 Published:2017-07-24

Abstract

Abstract: One-year-old wild Actinidia deliciosa seedlings were treated with exogenous melatonin solution in order to investigate the effects of electrolyte leakage, phenolic substances (total phenolics, total flavonoids and total flavanols) and antioxidant activities were evaluated, including ABTS^.+ radical scavenging capacity (ABTS), DPPH radical scavenging capacity (DPPH), and iron ion reducing ability (FRAP).The results showed that the relative electrolytic leakage was reduced, total phenolics and total flavonoids were significantly increased and antioxidant ability was improved. This suggested that MT pretreatment alleviated the damage by salt stress in kiwifruit seedlings, and thus improved the salt resistance.

Key words: kiwifruit, exogenous melatonin, salt stress, phenolic, antioxidant ability

CLC Number:

S663.9

GAO Fan, XIA Hui, YUAN Xuezhen, HUANG Shouyi, LIU Ji, LIANG Dong. Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress[J]. , 2017, 29(7): 1144-1150.

References

[1] 陈德明, 杨劲松. 土壤盐渍环境与养分管理[J]. 土壤学进展, 1995, 23(5): 7-13.
CHEN D M, YANG J S. Soil salinization environment and nutrient management[J]. Advance in Soil Science , 1995, 23(5): 7-13. (in Chinese)
[2] 赵宣, 韩霁昌, 王欢元,等. 盐渍土改良技术研究进展[J]. 中国农学通报, 2016, 32(8):113-116.
ZHAO X, HAN J C, WANG H Y, et al. Research progress of saline soil improvement technology[J]. Chinese Agricultural Science Bulletin , 2016, 32(8):113-116. (in Chinese with English abstract)
[3] 裘丽珍, 黄有军, 黄坚钦,等. 不同耐盐性植物在盐胁迫下的生长与生理特性比较研究[J]. 浙江大学学报(农业与生命科学版), 2006, 32(4):420-427.
QIU L Z, HUANG Y J, HUANG J Q, et al. Comparative study on vegetal and physiological characteristics of different salt-tolerant plants under salt stress[J]. Journal of Zhejiang University ( Agriculture & Life Sciences ), 2006, 32(4):420-427. (in Chinese with English abstract)
[4] JAMPEETONG A, BRIX H. Effects of NaCl salinity on growth, morphology, photosynthesis and proline accumulation of Salvinia natans [J]. Aquatic Botany , 2009, 91(3):181-186.
[5] LEA US, SLIMESTAD R, SMEDVING P, et al. Nitrogen deficiency enhances expression of specific MYB and bHLH transcription factors and accumulation of end products in the flavonoid pathway[J]. Planta , 2007, 225(5):1245-1253
[6] FINI A, GUIDI L, FERRINI F, et al. Drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in Fraxinus ornus leaves: An excess light stress affair?[J]. Journal of Plant Physiology , 2012, 169(10):929-939.
[7] 黄宏文. 猕猴桃研究进展[M].北京: 科学出版社, 2000: 1.
[8] 马凯, 汪良驹, 王业遴,等. 十八种果树盐害症状与耐盐性研究[J]. 果树科学, 1997, 14(1):1-5. (in Chinese)
MA K, WANG L J, WANG Y L, et al. Study on salt resistance and salt tolerance of 18 kinds of fruit trees[J]. Journal of Fruit Science , 1997, 14(1):1-5. (in Chinese)
[9] LERNER A B, CASE J D, TAKAHASHI Y, et al. Isolation of melatonin, the pineal gland factor that lightens melanocytes[J]. Journal of the American Chemical Society , 1958, 80(10):2587.
[10] HERNáNDEZRUIZ J, CANO A, ARNAO M B. Melatonin: a growth-stimulating compound present in lupin tissues.[J]. Planta , 2004, 220(1):140-144.
[11] HERNáNDEZRUIZ J, CANO A, ARNAO M B. Melatonin acts as a growth-stimulating compound in some monocot species.[J]. Journal of Pineal Research , 2005, 39(2):137-142.
[12] CHEN Q, QI W B R J, WEI W, et al. Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea[J]. Journal of Plant Physiology , 2009, 166(3):324.
[13] 徐芳,周海鹏,郭早霞,等.植物褪黑素及其抗逆性研究[J].基因组学与应用生物学,2013,32 (2):260-266.
XU F, ZHOU H P, GUO Z X, et al. The melatonin and its resistance to stress in plants[J]. Genomics and Applied Biology , 2013,32 (2):260-266. (in Chinese with English abstract)
[14] 杜天浩. 外源褪黑素对NaCl胁迫下番茄幼苗生理代谢和产量及品质的影响[D]. 杨凌:西北农林科技大学, 2016.
DU T H. Effects of exogenous melatonin on tomato seeldings physiology and friut yield and quality under NaCl stress[D]. Yangling: Northwest A&F University, 2016.
[15] 李超. 盐胁迫下不同抗盐苹果砧木响应的生理差异及褪黑素的缓解效应[D]. 杨陵: 西北农林科技大学, 2012.
LI C. Physiological differences of apple rootstocks with different salt tolerance response to salt stress and mitigation effects of melatonin[D]. Yangling: Northwest A&F University, 2012.
[16] 张海军. 褪黑素处理对氯化钠胁迫下黄瓜种子萌发的影响及其分子机制[D]. 北京:中国农业大学, 2016.
ZHANG H J. Regulation of melatonin on seed germination under salt stress in cucumber[D]. Beijing: China Agriculture University, 2016.
[17] 刘旭峰. 猕猴桃耐盐性比较试验[J]. 陕西农业科学, 1992 (1) : 19.
LIU X F. Comparative experiment on salt tolerance of kiwifruit[J]. Shaanxi Journal of Agricultural Sciences , 1992 (1) : 19.
[18] 周立名, 王飞, 王佳. EMS诱变处理定向筛选猕猴桃耐盐突变体研究[J]. 西北农业学报, 2009, 18 (5) : 330-335.
ZHOU L M, WANG F, WANG J. Selection of salt-tolerant mutants from ethyl methane sulfonate mutagenized kiwifruit embryonic calli[J]. Acta Agriculturae Boreali-Occidentalis Sinica , 2009, 18 (5) : 330-335. (in Chinese with English abstract)
[19] 陈建勋. 植物生理学实验指导[M]. 广州:华南理工大学出版社,2006.
[20] ZHANG K M, YU H J, SHI K, et al. Photoprotective roles of anthocyanins in Begonia semperflorens[J]. Plant Science , 2010,179(3): 202-208.
[21] SINGLETON V L, ROSSI J A. Colorimetry of total phenols with phosphomolybdic phosphotungstic acid reagents[J]. American Journal of Enology & Viticulture , 1965,16(3): 144-158.
[22] JIA Z, TANG M, WU J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J]. Food Chemistry , 1999, 64(4): 555-559.
[23] LI Y G, TANNER G, LARKIN P. The DMACA-HCl protocol and the threshold proanthocyanidin content for bloat safety in forage legumes[J]. Journal of the Science of Food & Agriculture , 1996,70(1): 89-101.
[24] BRANDWILLIAMS W, CUVELIER M E, BERSET C. Use of a free radical method to evaluate antioxidant activity[J]. LWT-Food Science and Technology , 1995, 28(1): 25-30.
[25] RE R, PELLEGRINI N, PROTEGGENTE A, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay[J] . Free Radical Biology & Medicine , 1999,26(10): 1231-1237.
[26] BENZIE I F, STRAIN J J. Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay[J]. Analytical Biochemistry , 1996, 239(1): 70-76.
[27] 李会珍, 张志军, 刘培培,等. 不同基因型紫苏耐盐性比较研究[J]. 西北植物学报, 2011, 31(2):338-344.
LI H Z, ZHANG Z J, LIU P P, et al. Comparative study on salt tolerance of different genotypical perilla[J]. Acta Botanica Boreali-Occidentalia Sinica , 2011, 31(2):338-344. (in Chinese with English abstract)
[28] 韩蕊莲, 李丽霞, 梁宗锁. 干旱胁迫下沙棘叶片细胞膜透性与渗透调节物质研究[J]. 西北植物学报, 2003, 23(1) :23-27.
HAN R L, LI L X, LIANG Z S. Seabuckthorn relative membrane conductivity and osmotic adjustment under drought stress[J]. Acta Botanica Boreali-Occidentalia Sinica , 2003, 23(1) :23-27. (in Chinese with English abstract)
[29] ZHU J K. Salt and drought stress signal transduction in plants.[J]. Plant Biology , 2002, 53(53):247-73.
[30] TAN D X, MANCHESTER L C, REITER R J, et al. Melatonin directly scavenges hydrogen peroxide: a potentially new metabolic pathway of melatonin biotransformation[J]. Free Radical Biology & Medicine , 2001, 29(11):1177-1185.
[31] LI C, WANG P, WEI Z, et al. The mitigation effects of exogenous melatonin on salinity-induced stress in Malus hupehensis [J]. Journal of Pineal Research , 2012, 53(3):298-306.
[32] GARCIA J, REITER R J, GUERRERO J M, et al. Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation[J]. FEBS Letters , 1997, 408(3):297-300.
[33] LEI X Y, ZHU R Y, ZHANG G Y, et al. Attenuation of cold-induced apoptosis by exogenous melatonin in carrot suspension cells: the possible involvement of polyamines.[J]. Journal of Pineal Research , 2004, 36(2):126-131.
[34] 陈立松, 刘星辉. 水分胁迫对荔枝叶片活性氧代谢的影响[J]. 园艺学报, 1998, 25(3):241-246.
CHEN L S, LIU X H. Effects on active oxygen metabolism of litchi under water stress[J]. Acta Horticulturae Sinica , 1998, 25(3):241-246. (in Chinese with English abstract)
[35] 潘东明, 郑国华. 水分胁迫与枇杷叶片SOD活性及脂质过氧化作用[J]. 福建农学院学报(自然版), 1993, 22(2):254-257.
PAN D M, ZHENG G H. Superoxide dismutase activity and lipid peroxidation in leaves of loquat under water stress[j]. Journal of Fujian Agrivultural College ( Natural Sciences Edition ), 1993, 22(2):254-257. (in Chinese with English abstract)
[36] RANDHIR R, LIN Y T, SHETTY K. Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors[J]. Process Biochemistry , 2004, 39(5):637-646.
[37] JAAKOLA L, HOHTOLA A. Effect of latitude on flavonoid biosynthesis in plants.[J]. Plant Cell & Environment , 2010, 33(8):1239-1247.
[38] SELMAR D, KLEINWäCHTER M. Influencing the product quality by deliberately applying drought stress during the cultivation of medicinal plants[J]. Industrial Crops & Products , 2013, 42(1):558-566.
[39] NAKABAYASHI R, YONEKURASAKAIBARA K, URANO K, et al. Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids[J]. Plant Journal , 2014, 77(3):367-379.
[40] YUAN Y, QI L, YANG J, et al. Erratum to: A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco[J]. Plant Cell , Tissue and Organ Culture , 2015, 120(3):973-973.
[41] WALIA H, WILSON C, CONDAMINE P, et al. Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage.[J]. Plant Physiology , 2005, 139(2):822-835.
[42] DA P C, CALLIGARIS S, CELOTTI E, et al. Antiradical properties of commercial cognacs assessed by the DPPH test[J]. Journal of Agricultural & Food Chemistry , 2000, 48(9):4241-4245.
[43] STANDLEY L, WINTERTON P, MAARNEWICK J L, et al. Influence of processing stages on antimutagenic and antioxidant potentials of rooibos tea[J]. Journal of Agricultural & Food Chemistry , 2001, 49(1):114-117.
[44] MILLER N J, RICE-EVANS C, DAVIES M J, et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates[J]. Clinical Science , 1993, 84(4):407-412.
[45] BUB A, WATZL B L, DELINCEE H, et al. Moderate intervention with carotenoid-rich vegetable products reduces lipid peroxidation in men[J]. Journal of Nutrition , 2000, 130(9):2200-2206.

Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress

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