›› 2017, Vol. 29 ›› Issue (6): 888-893.DOI: 10.3969/j.issn.1004-1524.2017.06.05

• Animal Science • Previous Articles     Next Articles

Drug resistance spectrum analysis and MLST genotyping of Escherichia coli from refrigerated chicken in three regions of Zhejiang Province

YANG Hua1,2, HE Xiangxiang1,2,3, XIAO Yingping1,2, QIAN Mingrong1,2, ZHANG Qiaoyan1, TANG Biao1,2,*   

  1. 1. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    3. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Received:2017-05-12 Online:2017-06-20 Published:2017-09-07

Abstract: Escherichia coli is a common opportunistic pathogen in food. Its drug resistance is severe in which the related resistance genes can easily spread. In this study, 59 strains of E. coli from refrigerated chicken in three regions of Zhejiang Province were isolated. Using drug resistant spectrum determination, 24 types of drug resistant spectrum were found, which showed that the drug resistant types of E. coli isolates were multifarious. Meanwhile, all the E. coli isolates were analyzed with MLST genotyping. The results showed that a total of 38 ST types were obtained, and two new ST types were found as well. On this basis, the phylogenetic analysis of 7 housekeeping genes showed a certain degree of the regional differences. This research results can provide scientific reference for the evaluation of drug resistance and traceability analysis of avian Escherichia coli in Zhejiang province, and a wider region in China.

Key words: Escherichia coli, bacterial drug resistance, multilocus sequence typing (MLST), phylogenetic tree

CLC Number: