Detoxification protocal optimization and virus detection in strawberry variety Hongyan

doi:10.3969/j.issn.1004-1524.2017.06.15

›› 2017, Vol. 29 ›› Issue (6): 966-970.DOI: 10.3969/j.issn.1004-1524.2017.06.15

• Plant Protection • Previous Articles Next Articles

Detoxification protocal optimization and virus detection in strawberry variety Hongyan

CHEN Ying, XIAO Chunlin, LUO Yanna, ZHANG Xiying, LIU Jiangna

Research Institute of Agricultural Sciences, the 6th Agricultural Production Division of Xinjiang Production and Construction Corps, Wujiaqu 831300, China

Received:2017-02-09 Online:2017-06-20 Published:2017-09-07

Abstract

Abstract: With strawberry variety Hongyan as the material, the sterile container seedlings were treated with high temperature treatment, low temperature freezing treatment combined with stem tip off detoxification. The double antibody step of serology and the clip art enzyme-linked immunoassay detection method were carried out to detect four kinds of virus. The survival rate and the detoxification efficiency of seedlings induced by meristem cells were compared. The results showed that the survival rate increased from 23.0% to 39.6%-60.0% under-1 ℃ cryogenic treatment with the stem tip stripping length increased from 0.3 mm to 0.5 mm, and all four virus were removed by the double antibody sandwich enzyme-linked immunoassay detection.

Key words: strawberry, temperature, virus-free meristem, virus detection, optimization

CLC Number:

S668.4

CHEN Ying, XIAO Chunlin, LUO Yanna, ZHANG Xiying, LIU Jiangna. Detoxification protocal optimization and virus detection in strawberry variety Hongyan[J]. , 2017, 29(6): 966-970.

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Detoxification protocal optimization and virus detection in strawberry variety Hongyan

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