Effect of Baicalin on expression of Toll-like receptor 2, 3, 4, 7 in Newcastle disease virus-infected CEFs

doi:10.3969/j.issn.1004-1524.2017.12.05

›› 2017, Vol. 29 ›› Issue (12): 1986-1993.DOI: 10.3969/j.issn.1004-1524.2017.12.05

• Animal Science • Previous Articles Next Articles

Effect of Baicalin on expression of Toll-like receptor 2, 3, 4, 7 in Newcastle disease virus-infected CEFs

XU Ruiguang¹, JIA Yan², HU Liwen¹, WENG Jiahua¹, DENG Junliang¹, HU Yanchun^{1, *}

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Agricultural and Environmental Branch, Jiaxing Vocational Technical College, Jiaxing 314036, China

Received:2017-05-22 Online:2017-12-20 Published:2018-01-08

Abstract

Abstract: The purpose of this study was to investigate the changes of the expression of TLR2, TLR3, TLR4 and TLR7 gene in NDV-infected cells and the effect of Baicalin on them. NDV infection of chicken embryo fibroblasts (CEFs) was performed. Cytotoxicity of Baicalin was tested by the MTT method. Quantitative expressions of TLR2, TLR3, TLR4 and TLR7 gene were analyzed by real-time fluorescence quantitative PCR (RTFQ PCR). The results showed that the expressions of TLR2, TLR4 and TLR7 genes were suppressed by NDV in the early infection stage, while their expression were enhanced with the replication of NDV and the expression of TLR2 and TLR4 gene reached the peak level at 24 h. As well as the expression of TLR7 gene reached the peak level at 16 h. The infection of NDV could change the expression of TLR3 gene, but it was not regular. Besides, Baicalin could increase the expression of TLR2 and TLR4 genes in NDV-infected cells and show the dosage-dependence, which enhanced immune function and possessed anti-NDV activity.

Key words: Baicalin, Newcastle disease virus (NDV), Toll-like receptors (TLRs), real-time fluorescence quantitative PCR (RTFQ PCR)

CLC Number:

S831.7

XU Ruiguang, JIA Yan, HU Liwen, WENG Jiahua, DENG Junliang, HU Yanchun. Effect of Baicalin on expression of Toll-like receptor 2, 3, 4, 7 in Newcastle disease virus-infected CEFs[J]. , 2017, 29(12): 1986-1993.

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Effect of Baicalin on expression of Toll-like receptor 2, 3, 4, 7 in Newcastle disease virus-infected CEFs

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