Isolation, identification and antibiotic sensitivity of Pseudomonas putida from hybrid sturgeon (Huso dauricus♀×Acipenser schrenckii♂)

doi:10.3969/j.issn.1004-1524.2017.12.04

›› 2017, Vol. 29 ›› Issue (12): 1978-1985.DOI: 10.3969/j.issn.1004-1524.2017.12.04

• Animal Science • Previous Articles Next Articles

Isolation, identification and antibiotic sensitivity of Pseudomonas putida from hybrid sturgeon (Huso dauricus♀×Acipenser schrenckii♂)

YANG Yuanyuan¹, YANG Yibin^{2, *}, CAO Haipeng³, FANG Wei¹, LIN Hanqun¹, YANG Xianle³, AI Xiaohui²

1. Guangdong Provincial Center for Aquatic Animal Disease Prevention and Control, Guangzhou 510222, China;
2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
3. Shanghai Ocean University, Shanghai 201306, China

Received:2017-04-11 Online:2017-12-20 Published:2018-01-08

Abstract

Abstract: The isolation, identification and antibiotic sensitivity of pathogen from the morbid hybrid sturgeon were conducted to provide a reference for control and prevention disease for hybrid sturgeon. The pathogenic bacteria were isolated and purified from liver, kidney, spleen and intestinal tract of hybrid sturgeon. The biochemical identification and 16S rRNA gene sequence determination and the artificial infection test were used to identify whether the strain XY4 was the pathogen, and the antimicrobial susceptibility test was conducted by K-B method. The results showed that strain XY4 was the pathogens of hybrid sturgeon, the LD₅₀ of the isolate was counted to 1.30×10⁶ cfu·g^-1. According to morphological and biochemical characteristics as well as the result of 16S rRNA gene sequence analysis, the isolated strain XY4 was Pseudomonas putida. Strain XY4 was susceptible to cefadroxil, florfenicol, doxycycline and other 6 kinds of antibiotics. The disease caused by Pseudomonas putida can be prevented by above sensitive drugs in fishery farming.

Key words: Pseudomonas putida, hybrid sturgeon(Huso dauricus♀×, Acipenser schrenckii♂), isolation and identification, antimicrobial susceptibility

CLC Number:

S941.42

YANG Yuanyuan, YANG Yibin, CAO Haipeng, FANG Wei, LIN Hanqun, YANG Xianle, AI Xiaohui. Isolation, identification and antibiotic sensitivity of Pseudomonas putida from hybrid sturgeon (Huso dauricus♀×Acipenser schrenckii♂)[J]. , 2017, 29(12): 1978-1985.

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Isolation, identification and antibiotic sensitivity of Pseudomonas putida from hybrid sturgeon (Huso dauricus♀×Acipenser schrenckii♂)

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