Isolation and identification of mycorrhizal fungi in rhizosphere and their effect on growth and photosynthetic characteristics of Eucalyptus globulus seedlings

doi:10.3969/j.issn.1004-1524.2022.05.16

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (5): 1015-1023.DOI: 10.3969/j.issn.1004-1524.2022.05.16

• Horticultural Science • Previous Articles Next Articles

Isolation and identification of mycorrhizal fungi in rhizosphere and their effect on growth and photosynthetic characteristics of Eucalyptus globulus seedlings

WANG Xiaoli¹(), ZHAO Yingwei², KONG Xiaona¹, CAO Zilin³^,^*()

1. College of Forestry, Southwest Forestry University, Kunming 650224, China
2. State-Owned Dongmen Forest Farm in Guangxi,Fusui 532108, Guangxi, China
3. College of Ecology and Environment, Southwest Forestry University, Kunming 650224, China

Received:2021-05-03 Online:2022-05-25 Published:2022-06-06
Contact: CAO Zilin

Abstract

Abstract:

In Eucalyptus, Eucalyptus globulus is a specific species for oil and timber dual-use. In order to provide theoretical basis and practical guidance for the cultivation of sound seedlings of E. globulus from the perspective of mycorrhizal seedlings, rhizosphere soil of E. globulus was collected from Yunnan Province. The species and dominant species of mycorrhizal fungi in rhizosphere soil were investigated. The effects of the isolated mycorrhizal fungi on the growth and photosynthetic characteristics of E. globulus seedlings were analyzed by controlling the number of mycorrhizal fungi in the nursery substrate. The main species of mycorrhizal fungi affecting seedling growth were explored through gradual regression analysis. The results showed that six species of mycorrhizal fungi were isolated from the rhizosphere soil of E. globulus plantation, which were Glomus mosseae, Glomus aggregatum, Glomus multicaule, Claroideoglomus etunicatum, Septoglomus constrictum, and Glomus hoi. The dominant mycorrhizal fungi were G. aggregatum, C. etunicatum and G. mosseae, which accumulatively accounted for 80.77% of the total spore density. At 10 months of age, the infection rate and infection intensity of mycorrhizal fungi of E. globulus seedlings were increased with the increase of the number of mycorrhizal fungi in the nursery substrate. At the ages of 5 months and 10 months, the seedling height, ground diameter of E. globulus seedlings were significantly (P<0.05) elevated with mycorrhizal fungi in the nursery substrate, the light compensation point and light saturation point of E. globulus seedlings were significantly (P<0.05) reduced, meanwhile the chlorophyll content and apparent quantum yield were significantly (P<0.05) increased. The dominant species promoting the growth and photosynthesis of E. globulus seedlings were G. aggregatum, C. etunicatum and S. constrictum, which was revealed by the correlation analysis and stepwise regression analysis.

Key words: Eucalyptus globulus Labill, mycorrhizal fungi, isolation and identification, growth index, photosynthetic characteristics

CLC Number:

WANG Xiaoli, ZHAO Yingwei, KONG Xiaona, CAO Zilin. Isolation and identification of mycorrhizal fungi in rhizosphere and their effect on growth and photosynthetic characteristics of Eucalyptus globulus seedlings[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1015-1023.

Figures/Tables 13

References 23

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处理 Treatment	菌根侵染率 Mycorrhizal infection rate/%	菌根侵染强度 Mycorrhizal infection intensity
1	0	Ⅰ
2	61.5	Ⅳ
3	79.5	Ⅴ

处理 Treatment	菌根侵染率 Mycorrhizal infection rate/%	菌根侵染强度 Mycorrhizal infection intensity
1	0	Ⅰ
2	61.5	Ⅳ
3	79.5	Ⅴ

处理 Treatment	5个月 Five months		10个月 Ten months
处理 Treatment	苗高 Seedling height/cm	地径 Ground diameter/mm	苗高 Seedling height/cm	地径 Ground diameter/mm	根干重 Root dry weight/g	茎干重 Stem dry weight/g	叶干重 Leaf dry weight/g
1	28.61±1.22 ab	1.36±0.08	32.82±1.18 b	2.63±0.07 ab	0.33±0.07	0.76±0.08	0.55±0.03
2	31.39±1.29 a	1.41±0.78	36.77±0.82 a	2.82±0.09 a	0.39±0.07	0.78±0.09	0.56±0.01
3	26.31±1.11 b	1.28±0.59	31.18±1.02 b	2.46±0.11 b	0.35±0.05	0.82±0.08	0.73±0.08

处理 Treatment	5个月 Five months		10个月 Ten months
处理 Treatment	苗高 Seedling height/cm	地径 Ground diameter/mm	苗高 Seedling height/cm	地径 Ground diameter/mm	根干重 Root dry weight/g	茎干重 Stem dry weight/g	叶干重 Leaf dry weight/g
1	28.61±1.22 ab	1.36±0.08	32.82±1.18 b	2.63±0.07 ab	0.33±0.07	0.76±0.08	0.55±0.03
2	31.39±1.29 a	1.41±0.78	36.77±0.82 a	2.82±0.09 a	0.39±0.07	0.78±0.09	0.56±0.01
3	26.31±1.11 b	1.28±0.59	31.18±1.02 b	2.46±0.11 b	0.35±0.05	0.82±0.08	0.73±0.08

处理 Treatment	叶绿素a Chlorophyll a/ (mg·g^-1)	叶绿素b Chlorophyll b/ (mg·g^-1)	总叶绿素 Total chlorophyll/ (mg·g^-1)	叶面积指数 Leaf area index	表观量子效率 Apparent quantum yield	最大净光合速率 Maximum net photosynthetic rate/ (μmol·m^-2·s^-1)	暗呼吸速率 Dark respiration rate/(μmol· m^-2·s^-1)	光补偿点 Light compensation point/(μmol· m^-2·s^-1)	光饱和点 Light saturation point/(μmol· m^-2·s^-1)
1	0.705± 0.049 b	0.236± 0.013	0.942± 0.054 b	1.32± 0.16	0.047± 0.010 c	4.726± 1.880	1.035± 0.178	20.010± 1.189 a	315.923± 32.767 a
2	0.712± 0.061 b	0.245± 0.003	0.957± 0.064 b	1.49± 0.12	0.070± 0.006 b	4.838± 0.743	1.071± 0.031	13.044± 0.716 b	265.894± 4.345 b
3	0.834± 0.048 a	0.271± 0.071	1.105± 0.061 a	1.50± 0.11	0.097± 0.005 a	4.910± 1.074	1.127± 0.061	8.781± 0.606 c	217.170± 20.564 c

Isolation and identification of mycorrhizal fungi in rhizosphere and their effect on growth and photosynthetic characteristics of Eucalyptus globulus seedlings

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指标Index	A	B	C	D	E	F
苗高Seedling height/cm	-0.991	1.000^*	-0.958	0.991	0.958	-0.835
地径Ground diameter/cm	0.679	-0.271	0.032	-0.439	0.032	0.036
根干重Rood dry weight/g	-0.929	0.992	-0.945	0.996	0.945	-0.956
茎干重Stem dry weight/g	0.996	-0.846	0.693	-0.928	-0.693	0.553
叶干重Leaf dry weight/g	0.928	-0.657	0.457	-0.781	-0.457	0.158
表观量子效率Apparent quantum yield	0.689	-0.234	0.046	-0.283	-0.046	-0.053
最大净光合速率	-0.961	0.693	-0.544	0.444	0.544	-0.390
Maximum net photosynthetic rate/(μmol·m^-2·s^-1)
暗呼吸速率Dark respiration rate/(μmol·m^-2·s^-1)	-0.744	0.310	-0.125	0.207	0.125	0.228
光补偿点Light compensation point(μmol·m^-2·s^-1)	-0.544	0.052	0.138	0.454	0.138	0.322
光饱和点Light saturation point/(μmol·m^-2·s^-1)	-0.649	0.181	0.008	0.335	0.008	0.298

苗木指标Indexes of seedling	回归方程Regression equation	r	R²	P
Y₁:苗高Seedling height/cm	Y₁=21.675+0.234X₂+0.100X₅	1.000	0.999	0.017
Y₂:根干重Rood dry weight/g	Y₂=0.237+0.001X₂+0.002X₄	0.998	0.995	0.045
Y₂:最大净光合速率Maximum net photosynthetic rate/(μmoL·m^-2·s^-1)	Y₃=4.410+0.004X₂+0.004X₄	0.998	0.995	0.045

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