Effects of arbuscular mycorrhizal fungi on plant roots and soil microenvironment under cadmium stress

doi:10.3969/j.issn.1004-1524.2022.05.19

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (5): 1039-1048.DOI: 10.3969/j.issn.1004-1524.2022.05.19

• Environmental Science • Previous Articles Next Articles

Effects of arbuscular mycorrhizal fungi on plant roots and soil microenvironment under cadmium stress

DU Hong¹(), LI Yupeng², CHENG Wen³, XIAO Rongying⁴, HU Peng⁵

1. College of Agricultural Engineering, Henan Vocational College of Agriculture, Zhengzhou 451450, China
2. Wheat Research Center,Nanyang Academy of Agricultural Sciences, Nanyang 473000, Henan, China
3. Anhui Provincial Key Laboratory of Nutrient Recycling,Resources and Environment, Institute of Soil and Fertilizer,Anhui Academy of Agricultural Sciences, Hefei 230031, China
4. College of Agriculture,Xinyang Agriculture and Forestry University,Xinyang 464000, Henan, China
5. Microelement Research Center, College of Resources and Environment,Huazhong Agricultural University, Wuhan 430070, China

Received:2021-03-08 Online:2022-05-25 Published:2022-06-06

Abstract

Abstract:

In order to explore the effects of arbuscular mycorrhizal fungi (AMF) on plant roots and soil microorganism,a pot experiment was carried out with Lolium perenne as test plant, and Glomus versiforme as test AMP. Two factors were set: whether Lolium perenne was inoculated with AMF, and Cd concentration (0, 10, 20, 30 mg·kg^-1). It was shown that AMF infection rate and spore number were significantly (P<0.05) decreased with the increasing Cd concentration.Under the same Cd concentration, inoculation of AMF significantly (P<0.05) improved the root architecture (total root length, total root projection area, total root volume, number of root tips and number of root bifurcations) of Lolium perenne.Under the same Cd concentration, inoculation of AMF significantly (P<0.05) increased the number of fungi, bacteria, actinomycetes in soil,contents of soil microbial biomass carbon, soil microbial biomass nitrogen,soil polyphenol oxidase activity, and the contents of easily extractable glomalin-related soil protein and total glomalin-related soil protein. Therefore, it was concluded that AMF could increase plant resistance by improving plant roots and microbial environment, and increasing soil related enzyme activities and glomus content.

Key words: arbuscular mycorrhizal fungi, cadmium stress, soil microorganism, root system

CLC Number:

S154.39

DU Hong, LI Yupeng, CHENG Wen, XIAO Rongying, HU Peng. Effects of arbuscular mycorrhizal fungi on plant roots and soil microenvironment under cadmium stress[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1039-1048.

Figures/Tables 8

References 43

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接种 Inoculation	Cd浓度 Cd concentration/(mg·kg^-1)	菌根侵染率 Colonization rate/%	孢子数 Number of spores/cm^-1	株高 Plant height/cm	地上部干重 Dry weigh of shoot/g
NM	0	—	—	34.8±2.2 bc	3.609±0.386 a
	10	—	—	30.8±2.4 de	2.623±0.198 c
	20	—	—	28.0±1.8 ef	2.510±0.134 cd
	30	—	—	22.9±1.3 g	2.138±0.180 d
AMF	0	53.0±7.5 a	15.3±3.1 a	42.6±2.3 a	3.897±0.282 a
	10	47.6±2.5 ab	12.3±2.5 ab	37.3±1.8 b	3.507±0.346 ab
	20	42.7±2.5 bc	10.7±1.2 bc	32.0±2.3 cd	3.125±0.205 b
	30	37.7±2.5 c	7.3±0.6 c	25.9±1.3 fg	2.406±0.261 cd

接种 Inoculation	Cd浓度 Cd concentration/(mg·kg^-1)	菌根侵染率 Colonization rate/%	孢子数 Number of spores/cm^-1	株高 Plant height/cm	地上部干重 Dry weigh of shoot/g
NM	0	—	—	34.8±2.2 bc	3.609±0.386 a
	10	—	—	30.8±2.4 de	2.623±0.198 c
	20	—	—	28.0±1.8 ef	2.510±0.134 cd
	30	—	—	22.9±1.3 g	2.138±0.180 d
AMF	0	53.0±7.5 a	15.3±3.1 a	42.6±2.3 a	3.897±0.282 a
	10	47.6±2.5 ab	12.3±2.5 ab	37.3±1.8 b	3.507±0.346 ab
	20	42.7±2.5 bc	10.7±1.2 bc	32.0±2.3 cd	3.125±0.205 b
	30	37.7±2.5 c	7.3±0.6 c	25.9±1.3 fg	2.406±0.261 cd

接种 Inoculation	Cd浓度 Cd concentration/ (mg·kg^-1)	根系总长度 Total root length/cm	根系总投影面积 Total root projection area/cm²	根系总体积 Total root volume/cm³	根尖数 Number of root tips	根分叉数 Number of root branches
NM	0	365.0±8.8 b	31.3±1.1 b	0.159±0.004 a	2 080.3±225.5 ab	1 378.0±167.9 ab
	10	305.3±14.3 d	23.1±3.1 cd	0.124±0.009 b	1 604.0±165.4 cd	1 129.0±105.5 cd
	20	203.3±13.0 ef	18.6±3.0 e	0.086±0.011 c	1 251.0±96.6 ef	771.7±130.5 ef
	30	153.7±19.7 g	11.9±1.2 f	0.062±0.015 d	1 045.7±71.6 f	567.3±59.9 f
AMF	0	399.7±20.9 a	38.7±2.9 a	0.169±0.004 a	2 149.0±266.1 a	1 562.3±117.8 a
	10	334.2±12.6 c	32.8±2.5 b	0.134±0.010 b	1 814.0±174.8 bc	1 234.7±101.4 bc
	20	230.6±15.0 e	25.1±1.7 c	0.102±0.011 c	1 371.7±118.6 de	925.0±122.3 de
	30	203.9±16.1 ef	19.6±1.1 de	0.092±0.015 c	1 080.0±131.4 f	741.3±111.5 ef

接种 Inoculation	Cd浓度 Cd concentration/ (mg·kg^-1)	根系总长度 Total root length/cm	根系总投影面积 Total root projection area/cm²	根系总体积 Total root volume/cm³	根尖数 Number of root tips	根分叉数 Number of root branches
NM	0	365.0±8.8 b	31.3±1.1 b	0.159±0.004 a	2 080.3±225.5 ab	1 378.0±167.9 ab
	10	305.3±14.3 d	23.1±3.1 cd	0.124±0.009 b	1 604.0±165.4 cd	1 129.0±105.5 cd
	20	203.3±13.0 ef	18.6±3.0 e	0.086±0.011 c	1 251.0±96.6 ef	771.7±130.5 ef
	30	153.7±19.7 g	11.9±1.2 f	0.062±0.015 d	1 045.7±71.6 f	567.3±59.9 f
AMF	0	399.7±20.9 a	38.7±2.9 a	0.169±0.004 a	2 149.0±266.1 a	1 562.3±117.8 a
	10	334.2±12.6 c	32.8±2.5 b	0.134±0.010 b	1 814.0±174.8 bc	1 234.7±101.4 bc
	20	230.6±15.0 e	25.1±1.7 c	0.102±0.011 c	1 371.7±118.6 de	925.0±122.3 de
	30	203.9±16.1 ef	19.6±1.1 de	0.092±0.015 c	1 080.0±131.4 f	741.3±111.5 ef

接种 Inoculation	Cd浓度 Cd concentration/(mg·kg^-1)	真菌 Fungi/(10⁴ g^-1)	细菌 Bacteria/(10⁵CFU·g^-1)	放线菌 Actinomyces/(10⁵ g^-1)
NM	0	1.45±0.19 cd	1.73±0.07 c	4.26±0.39 b
	10	1.24±0.13 de	1.46±0.05 cd	3.43±0.18 c
	20	1.13±0.11 e	1.31±0.12 d	2.46±0.19 de
	30	1.06±0.10 e	1.23±0.08 d	2.32±0.15 e
AMF	0	2.51±0.31 a	3.10±0.19 a	5.17±0.29 a
	10	2.06±0.09 b	2.35±0.27 b	4.13±0.44 b
	20	1.59±0.07 c	1.73±0.31 c	3.05±0.63 cd
	30	1.22±0.05 de	1.41±0.13 d	3.17±0.25 c

Effects of arbuscular mycorrhizal fungi on plant roots and soil microenvironment under cadmium stress

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接种 Inoculation	Cd浓度 Cd concentration/ (mg·kg^-1)	脱氢酶 Dehydrogenase activity/(mg·g^-1)	酸性磷酸酶 Acid phosphatase activity/(mg·g^-1)	过氧化氢酶 Catalase activity/ (mg·g^-1)	多酚氧化酶 Polyphenol oxidase activity/(mg·g^-1)
NM	0	0.693±0.134 d	53.4±6.6 a	0.803±0.165 d	0.132±0.020 b
	10	0.797±0.162 cd	42.8±2.7 bcd	1.027±0.090 cd	0.093±0.030 c
	20	0.840±0.195 bcd	39.5±3.5 cde	1.240±0.082 abc	0.063±0.010 d
	30	0.970±0.080 abc	34.2±3.3 ef	1.333±0.061 ab	0.060±0.010 d
AMF	0	0.797±0.163 cd	47.5±4.4 ab	1.090±0.134 c	0.163±0.020 a
	10	0.853±0.120 bcd	45.7±4.5 bc	1.193±0.146 bc	0.125±0.020 b
	20	1.043±0.066 ab	36.9±3.0 def	1.437±0.190 a	0.105±0.020 bc
	30	1.087±0.129 a	30.9±1.3 f	1.450±0.177 a	0.076±0.020 cd

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[2]	HUANG Yongming, SONG Fang, WANG Ce, YAO Jinglei, WANG Zhijing, HE Ligang, WU Liming, JIANG Yingchun. Effects of root pruning on growth and expression of related genes in Poncirus trifoliata [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 270-277.
[3]	ZHAO Hua, REN Qingwen, WANG Xiyu, LI Zhenni, TANG Xiumei, JIANG Lihui, LIU Peng, XING Chenghua. Effects of arbuscular mycorrhizal fungi on antioxidant enzymes activities and photosynthetic characteristics of Solanum lycopersicum L. under salt stress [J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2075-2084.
[4]	GE Jintao, WANG Jiangying, ZHAO Wenjing, SHAO Xiaobin, ZHU Pengbo, TANG Xueyan, SUN Mingwei, LIU Xingman. Transcriptome analysis on development of aerial root in grape of Weike [J]. , 2020, 32(9): 1645-1655.
[5]	LIN Hui, ZHANG Jin, YUAN Qianyu, YE Jing, SUN Wanchun, YU Yijun, YU Qiaogang, MA Junwei. Improving microbial system of continuous cropping soil by addition of Trichoderma asperellum and ultrafine powder humus [J]. , 2020, 32(6): 1060-1069.
[6]	LIU Tao, ZHANG Chipeng, HAO Yaoling, QIU Lijuan, HUANG Chenchen. Effects of sulfate on reduction and transformation of soil iron minerals and arsenic release [J]. , 2020, 32(4): 678-684.
[7]	WANG Baojun, CHENG Wangda, CHEN Gui, SHEN Yaqiang, SHEN Meng, YUAN Ye, WANG Lei, ZHANG Hongmei. Effects of nitrogen fertilizer regulation on soil properties of paddy fields and rice yield with full amount returning of straw in Northern Zhejiang [J]. , 2020, 32(2): 183-190.
[8]	SHI Zhaoyong, LI Ke, WANG Fayuan, WANG Xugang, XU Xiaofeng. Effects of nano-silver and exotic arbuscular mycorrhizal fungi on chlorophyll fluorescence kinetics of sweet sorghum [J]. , 2020, 32(2): 283-290.
[9]	TAO Jing, WU Qifeng, SHI Jiang, LI Songhao, GE Jiangfei, CHEN Junhui, XU Qiufang, LIANG Chenfei, QIN Hua. Impact of intercropping and arbuscular mycorrhizal fungi on soil fertility and corn yield in a newly cultivated mountain land [J]. , 2020, 32(1): 115-123.
[10]	WANG Qingxia, CHEN Xijing, YU Man, SHEN Alin. Research progress on effects of straw returning on nitrogen cycling microbes and functional genes in paddy soil [J]. , 2019, 31(2): 333-342.
[11]	LIANG Jingang, JIAO Yue, LIU Pengcheng, ZHANG Xiujie. Arbuscular mycorrhizal fungi as a potential indicator to assess effects of genetically modified crops on soil microorganisms [J]. , 2018, 30(7): 1267-1272.
[12]	JIANG Wu, WU Zhigang, CHEN Songlin, TAO Zhengming. Kinetic analysis and antioxidant activity of Dendrobium officinale leaves under cadmium stress [J]. , 2017, 29(9): 1421-1429.
[13]	GUO Jiang\\|bo;TANG Bing;WANG Jian\\|ying;CAI Lu;XIN Cui\\|hua*. Influence of cadmium stress on physiological indexes in tobacco [J]. , 2013, 25(6): 0-1283.
[14]	BAI Peng\\|hua;LIU Qi\\|zhi;*;LI Hong\\|xyu;LIU Yan\\|bin;SHI Wang\\|peng;WANG Wei;LIU Zhi\\|long;ZHOU Hai\\|ying;ZHAO Ming\\|xin. Relationships between leaf yellowing and soil microorganisms/nutrients in pear orchards [J]. , 2013, 25(6): 0-1336.