Isolation, identification and genetic evolution analysis of novel goose astrovirus in Anhui Province, China

doi:10.3969/j.issn.1004-1524.2023.05.08

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (5): 1048-1057.DOI: 10.3969/j.issn.1004-1524.2023.05.08

• Animal Science • Previous Articles Next Articles

Isolation, identification and genetic evolution analysis of novel goose astrovirus in Anhui Province, China

JIA Beiping¹(), LYU Xuan¹, YANG Qing¹, WANG Yinan¹, LI Wanxiao¹, XIE Xindi¹, ZHU Yingqi¹, WANG Bei¹, YIN Dongdong², ZHANG Yunkai³, WANG Qing¹, WANG Guijun¹^,^*()

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
2. Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230001, China
3. Hefei Huameng Biotechnology Co., Ltd., Hefei 231131, China

Received:2022-05-28 Online:2023-05-25 Published:2023-06-01

Abstract

Abstract:

To reveal the etiological and genetic characteristics of the novel goose astrovirus epidemic strains causing goose gout in recent years, the samples were collected from goslings suspected to be infected with the novel goose astrovirus in Anhui Province, China. The viruses were isolated by using healthy goose embryos, and the whole genome sequences of the isolated viruses were determined and analyzed. The results showed that five samples infected with novel goose astrovirus were identified. Five strains of goose astrovirus were successfully isolated from goose embryos by blind transmission for 3 generations. The strains were named as AHAU2018, DY-19, ZY02, HR2105/2 and HR2100/1, respectively. The whole genome sequencing results showed that the total genome length of the isolated strains was 7 175 nt. Phylogenetic tree analysis showed that the five isolated strains belonged to the novel goose astrovirus causing goose gout, and the novel goose astrovirus isolated before 2019 and after 2019 were in different evolutionary sub-branches. The novel goose astrovirus has been mutated in China, and new evolutionary sub branches have emerged. But, whether the dominant genotype has changed remains to be further studied.

Key words: novel goose astrovirus, isolation and identification, whole genome sequencing, genetic evolutionary analysis

CLC Number:

S852.657

JIA Beiping, LYU Xuan, YANG Qing, WANG Yinan, LI Wanxiao, XIE Xindi, ZHU Yingqi, WANG Bei, YIN Dongdong, ZHANG Yunkai, WANG Qing, WANG Guijun. Isolation, identification and genetic evolution analysis of novel goose astrovirus in Anhui Province, China[J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1048-1057.

Figures/Tables 10

References 33

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编号 No.	分离毒株 Virus strain	临床病例时间 Clinical case date	品种 Variety	送检日龄 Age in days/d	死亡率 Death rate/%
1	AHAU2018	2018年5月 May, 2018	朗德鹅Landes	10	77.8(1 400/1 800)
2	DY19	2019年9月 September, 2019	泰州白鹅Taizhou white	14	23.5(400/1 700)
3	ZY02	2021年4月 April, 2021	皖西白鹅Wanxi white	40	5.0~10.0
4	HR2105/2	2021年5月 May, 2021	朗德鹅Landes	17	40.0(1 000/2 500)
5	HR2110/1	2021年10月 October, 2021	朗德鹅Landes	20	70.4(2 174/3 090)

编号 No.	分离毒株 Virus strain	临床病例时间 Clinical case date	品种 Variety	送检日龄 Age in days/d	死亡率 Death rate/%
1	AHAU2018	2018年5月 May, 2018	朗德鹅Landes	10	77.8(1 400/1 800)
2	DY19	2019年9月 September, 2019	泰州白鹅Taizhou white	14	23.5(400/1 700)
3	ZY02	2021年4月 April, 2021	皖西白鹅Wanxi white	40	5.0~10.0
4	HR2105/2	2021年5月 May, 2021	朗德鹅Landes	17	40.0(1 000/2 500)
5	HR2110/1	2021年10月 October, 2021	朗德鹅Landes	20	70.4(2 174/3 090)

引物名称 Prime name	引物序列 Prime sequences(5'→3')	引物位置 Primer location
GASTV-0-F	AAACAGCGATATGGCGGC	1~18
GASTV-0-R	TGTACGACTTAGTCCAAGGAACG	823~845
GASTV-1-F	CACAGAGGCTACAATGCT	378~395
GASTV-1-R	CCTTCAACAACGACAATGG	1 665~1 683
GASTV-2-F	CAGTCCCTGTACAGATTTTA	1 446~1 465
GASTV-2-R	TCAACTTGTTCATCCTTTAC	2 792~2 811
GASTV-3-F	AGATTGATGAAGCCATTGAG	2 604~2 623
GASTV-3-R	CAGCCCGCCGTTCTGTCTGT	3 939~3 958
GASTV-4-F	AGGCTGTATCAGATATTGAT	3 755~3 774
GASTV-4-R	TCATTTTGTCATTAACGGG	5 014~5 032
GASTV-5-F	TCTGGTGAGTGGCGGACCGA	4 799~4 818
GASTV-5-R	TGTCATAACAGCCCACCAATTGTGT	6 273~6 297
GASTV-6-F	ACAACTGGACAAGGTACC	6 028~6 045
GASTV-6-R	TTTGCGGATTTTAAATGC	7 131~7 148

引物名称 Prime name	引物序列 Prime sequences(5'→3')	引物位置 Primer location
GASTV-0-F	AAACAGCGATATGGCGGC	1~18
GASTV-0-R	TGTACGACTTAGTCCAAGGAACG	823~845
GASTV-1-F	CACAGAGGCTACAATGCT	378~395
GASTV-1-R	CCTTCAACAACGACAATGG	1 665~1 683
GASTV-2-F	CAGTCCCTGTACAGATTTTA	1 446~1 465
GASTV-2-R	TCAACTTGTTCATCCTTTAC	2 792~2 811
GASTV-3-F	AGATTGATGAAGCCATTGAG	2 604~2 623
GASTV-3-R	CAGCCCGCCGTTCTGTCTGT	3 939~3 958
GASTV-4-F	AGGCTGTATCAGATATTGAT	3 755~3 774
GASTV-4-R	TCATTTTGTCATTAACGGG	5 014~5 032
GASTV-5-F	TCTGGTGAGTGGCGGACCGA	4 799~4 818
GASTV-5-R	TGTCATAACAGCCCACCAATTGTGT	6 273~6 297
GASTV-6-F	ACAACTGGACAAGGTACC	6 028~6 045
GASTV-6-R	TTTGCGGATTTTAAATGC	7 131~7 148

分离年份 Year	分离毒株 Virus strain	核苷酸同源性Nucleotide homology/%
		AHAU 2018			DY-19			HR2105/2			HR2110/1			ZY02
		ORF1a	ORF1b	ORF2	ORF1a	ORF1b	ORF2	ORF1a	ORF1b	ORF2	ORF1a	ORF1b	ORF2	ORF1a	ORF1b	ORF2
2016	JSHA	97.6	99.1	98.8	97.7	98.9	98.3	97.5	98.1	97.9	97.3	98.5	98.0	97.5	98.3	97.7
2017	SD01	98.8	99.0	98.5	98.4	98.8	98.1	97.8	98.1	97.7	97.8	98.5	97.8	98.0	98.3	97.4
	GD	97.8	99.0	98.4	98.1	98.8	98.0	97.8	98.0	97.6	97.6	98.4	97.6	97.7	98.2	97.4
	SDPY	98.0	99.0	98.9	98.2	99.0	98.7	97.8	98.2	98.2	97.7	98.6	98.2	97.9	98.4	98.0
2018	AHAU2018	—	—	—	98.1	99.0	98.4	97.7	98.4	98.0	97.6	98.6	98.0	97.8	98.6	97.7
	CXZ	97.8	99.0	99.1	97.8	98.8	98.7	97.5	98.1	98.3	97.3	98.5	98.3	97.6	98.3	98.0
	GTF-04	97.9	98.9	97.8	97.7	98.7	97.6	97.4	98.1	97.1	97.3	98.3	97.3	97.5	98.3	96.9
	GTF-07	97.8	98.6	98.4	97.7	98.4	98.0	97.4	97.6	97.5	97.3	98.0	97.5	97.5	97.8	97.3
	AHAU1	97.8	98.8	98.9	97.9	98.8	98.5	97.5	98.0	98.1	97.4	98.4	98.1	97.5	98.2	97.9
	AHAU4	98.0	98.8	98.9	98.1	98.7	98.5	97.7	97.9	98.1	97.6	98.3	98.1	97.7	98.1	97.8
	XX	98.8	99.4	98.4	98.4	99.2	98.0	98.0	98.4	97.7	97.9	98.8	97.6	98.1	98.6	97.5
2019	DY-19	98.1	99.0	98.4	—	—	—	99.2	98.8	98.3	99.2	99.5	98.9	98.9	99.0	98.1
	SDXT	97.5	98.7	99.0	97.5	98.5	98.7	97.1	97.7	98.2	97.0	98.1	98.2	97.2	97.9	97.9
	HBXG	97.9	98.9	98.8	98.1	98.7	98.4	97.7	97.9	98.0	97.6	98.3	98.2	97.8	98.1	97.8
2020	HNKF-1	97.9	98.8	98.7	99.6	99.7	99.5	99.2	98.6	98.6	99.2	99.5	99.1	98.9	98.8	98.4
	HNSQ-6	98.0	98.9	98.8	99.4	99.7	99.5	99.1	98.7	98.7	99.0	99.4	99.1	98.7	98.9	98.4
2021	HR2105/1	97.7	98.8	98.2	99.2	99.6	98.7	98.7	98.6	98.8	99.4	99.5	98.6	98.8	98.8	98.5
	HR2105/2	97.7	98.4	98.0	98.9	98.8	98.3	—	—	—	98.8	98.5	98.2	99.8	99.8	99.6
	HR2110/1	97.6	98.6	98.0	99.2	99.5	98.9	99.4	98.5	98.2	—	—	—	98.8	98.6	98.0
	ZY02	97.8	98.6	97.7	98.9	99.0	98.1	98.8	99.8	99.6	98.8	98.6	98.0	—	—	—
	ZM	97.9	98.5	98.1	99.0	98.9	98.4	99.0	99.7	99.8	99.0	98.5	98.3	99.8	99.9	99.6

Isolation, identification and genetic evolution analysis of novel goose astrovirus in Anhui Province, China

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[10]	BA Shaobo, SHI Lin, LI Qunjing, LIU Zhengkui, CHEN Lin, WANG Xiaodu, SONG Houhui. Isolation, identification and molecullar epidemiological analysis of 17-ZJ-HZ strain of porcine reproductive and respiratory syndrome virus [J]. , 2018, 30(8): 1303-1311.
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[12]	ZHENG Liping, GENG Yi, LEI Xueping, YU Zehui, HUANG Xiaoli, CHEN Defang, OUYANG Ping, CAO Shiqi, HAN Rui. Isolation and identification of Streptococcus iniae from sturgeon and its pathological lesions of infection [J]. , 2018, 30(2): 203-210.
[13]	LIAO Changyu, ZHANG Pengfei, WANG Yin, YANG Zexiao, YAO Xueping, JIANG Ruijiao, WU Xulong, ZHANG Bo, ZHOU Lijun, SONG Yong. Identification and biological characteristics analysis of Moraxella from swine [J]. , 2018, 30(1): 36-42.
[14]	MENG Zhengqun, LENG Yiyi, REN Meishen, LIU Yadong, WANG Yin, YAO Xueping, YANG Zexiao. Isolation, identification and drug resistance detection of a calf Klebsiella pneumoniae strain [J]. , 2017, 29(4): 534-541.
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