Effect of RNA interference with PhCatC1/2 gene on expression of related immune genes in Panulirus homarus

doi:10.3969/j.issn.1004-1524.2023.05.07

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (5): 1037-1047.DOI: 10.3969/j.issn.1004-1524.2023.05.07

• Animal Science • Previous Articles Next Articles

Effect of RNA interference with PhCatC1/2 gene on expression of related immune genes in Panulirus homarus

LIANG Feishuang(), LIANG Huafang(), Huang Jiayu, WANG Panmei, WEN Chongqing

Fisheries College, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China

Received:2021-12-27 Online:2023-05-25 Published:2023-06-01

Abstract

Abstract:

Preliminary study of the effect of RNA interference with the PhCatC1/2 gene on the expression of immune genes associated with Panulirus homarus,the expression of PhCatC1/2, PhTlr13, PhLys and Phβ-G genes were detected by qPCR and changes in hepatopancreatic tissue were observed. The results showed that the expression of PhCatC2 was significantly different from the control group after PhCatC1-dsRNA injection (P<0.05), the PhTlr13 gene expression showed a significant increase in the first period and then a decrease compared with the control group,expression of both PhLys and Phβ-G genes increased with increasing duration of dsRNA interference. Expression of PhTlr13 and Phβ-G genes increased with increasing duration of dsRNA interference after silencing of the PhCatC2 gene, PhCatC1 gene expression showed an alternating trend of down- and up-regulation, PhLys gene expression showed a trend of down-regulation followed by up-regulation. Preliminary speculation from analysis of the PhCatC1/2 gene after silencing that the lobster Panulirus homarus may be involved in the immune response by promoting Toll-like receptor and lysosomal pathways and concluding that the PhCatC1/2 gene has an immune function.

Key words: Panulirus homarus, RNA interference, PhCatC1/2, PhTlr13, PhLys, Phβ-G, expression analysis

CLC Number:

S917

LIANG Feishuang, LIANG Huafang, Huang Jiayu, WANG Panmei, WEN Chongqing. Effect of RNA interference with PhCatC1/2 gene on expression of related immune genes in Panulirus homarus[J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1037-1047.

Figures/Tables 6

References 51

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引物名称 Primer name	引物序列 Primer sequence(5'→3')	用途 Application
EGFP-F	ATGGTGAGCAAGGGCGAGGAG
EGFP-R	TTACTTGTACAGCTCGTCCATGCC
dsRNA-EGFP-F	TAATACGACTCACTATAGGGATGGTGAGCAAGGGCGAGGAG	dsRNA合成dsRNA synthesis
dsRNA-EGFP-R	TAATACGACTCACTATAGGGTTACTTGTACAGCTCGTCCATGCC	dsRNA合成dsRNA synthesis
PhCatC1-F	TCCTGGAGACGGAACGCTT	qPCR
PhCatC1-R	CCCTGGGATAACACTGAACACG
dsRNA-PhCatC1-F	TAATACGACTCACTATAGGGTCCTGGAGACGGAACGCTT	dsRNA合成dsRNA synthesis
dsRNA-PhCatC1-R	TAATACGACTCACTATAGGGCCCTGGGATAACACTGAACACG	dsRNA合成dsRNA synthesis
PhCatC2-F	CGACCTCCTATTGTGACCGC	qPCR
PhCatC2-R	TGACCAACGCCTCCAGCAT
dsRNA-PhCatC2-F	TAATACGACTCACTATAGGGCGACCTCCTATTGTGACCGC	dsRNA合成dsRNA synthesis
dsRNA-PhCatC2-R	TAATACGACTCACTATAGGGTGACCAACGCCTCCAGCAT	dsRNA合成dsRNA synthesis
β-actin-F	CCATCCAGGCTGTGCTCTCT	内参基因Reference gene
β-actin-R	GCGGTGGTTGTTGAAGGTG
PhTlr13-F	AGTCCTCTTGCCCACCTTACA	qPCR
PhTlr13-R	TCGGATGACCACTCGCTAACT
PhLys-F	TGTCTCCTCCTGGCTGTTCT	qPCR
PhLys-R	CCCACCCATCATTTCTGC
Phβ-G -F	TGTGGGTCTCAGTGGTTTTGG	qPCR
Phβ-G -R	GGTCTGGTTGGATCTGCTTCTT

引物名称 Primer name	引物序列 Primer sequence(5'→3')	用途 Application
EGFP-F	ATGGTGAGCAAGGGCGAGGAG
EGFP-R	TTACTTGTACAGCTCGTCCATGCC
dsRNA-EGFP-F	TAATACGACTCACTATAGGGATGGTGAGCAAGGGCGAGGAG	dsRNA合成dsRNA synthesis
dsRNA-EGFP-R	TAATACGACTCACTATAGGGTTACTTGTACAGCTCGTCCATGCC	dsRNA合成dsRNA synthesis
PhCatC1-F	TCCTGGAGACGGAACGCTT	qPCR
PhCatC1-R	CCCTGGGATAACACTGAACACG
dsRNA-PhCatC1-F	TAATACGACTCACTATAGGGTCCTGGAGACGGAACGCTT	dsRNA合成dsRNA synthesis
dsRNA-PhCatC1-R	TAATACGACTCACTATAGGGCCCTGGGATAACACTGAACACG	dsRNA合成dsRNA synthesis
PhCatC2-F	CGACCTCCTATTGTGACCGC	qPCR
PhCatC2-R	TGACCAACGCCTCCAGCAT
dsRNA-PhCatC2-F	TAATACGACTCACTATAGGGCGACCTCCTATTGTGACCGC	dsRNA合成dsRNA synthesis
dsRNA-PhCatC2-R	TAATACGACTCACTATAGGGTGACCAACGCCTCCAGCAT	dsRNA合成dsRNA synthesis
β-actin-F	CCATCCAGGCTGTGCTCTCT	内参基因Reference gene
β-actin-R	GCGGTGGTTGTTGAAGGTG
PhTlr13-F	AGTCCTCTTGCCCACCTTACA	qPCR
PhTlr13-R	TCGGATGACCACTCGCTAACT
PhLys-F	TGTCTCCTCCTGGCTGTTCT	qPCR
PhLys-R	CCCACCCATCATTTCTGC
Phβ-G -F	TGTGGGTCTCAGTGGTTTTGG	qPCR
Phβ-G -R	GGTCTGGTTGGATCTGCTTCTT

Effect of RNA interference with PhCatC1/2 gene on expression of related immune genes in Panulirus homarus

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