Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd

doi:10.3969/j.issn.1004-1524.2018.01.08

›› 2018, Vol. 30 ›› Issue (1): 58-64.DOI: 10.3969/j.issn.1004-1524.2018.01.08

• Horticultural Science • Previous Articles Next Articles

Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd

LIU Ziji¹, ZHU Jie², NIU Yu¹, YANG Yan^{1, *}

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, P. R. China, Danzhou 571737, China;
2. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China

Received:2017-06-07 Online:2018-01-20 Published:2018-02-09

Abstract

Abstract: In order to explore the expression regulation of α-momorcharin gene, the upstream sequence, 1 620 bp in length including start codon of α-momorcharin gene was cloned in bitter gourd (Y5). The 1 500 bp upstream of the transcription start site was selected for analysis based on PlantCARE promoter prediction tools. The results showed that in addition to containing the core elements of TATA-box and CAAT-box, the α-momorcharin promoter also contained light response elements, gibberellin response element, heat stress response element, drought response element, salicylic acid response element, jasmonic acid response element etc. Taking 28 bitter gourd germplasm as materials, the SNP and InDel distribution in promoter region of α-momorcharin was analyzed. Twenty-four SNP loci and 1 InDel were found. Four haplotypes were identified in α-momorcharin gene promoter through SNP and InDel grouping. Three SNPs were located in transcription factor binding sites, which may play important roles in regulating the expression of α-momorcharin gene.

Key words: bitter gourd, α-momorcharin;, promoter, haplotype

CLC Number:

S652.9

LIU Ziji, ZHU Jie, NIU Yu, YANG Yan. Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd[J]. , 2018, 30(1): 58-64.

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Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd

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