Polymorphism analysis of TGF-β2 gene promoter of chickens

doi:10.3969/j.issn.1004-1524.2016.12.10

›› 2016, Vol. 28 ›› Issue (12): 2028-2032.DOI: 10.3969/j.issn.1004-1524.2016.12.10

• Animal Science • Previous Articles Next Articles

Polymorphism analysis of TGF-β2 gene promoter of chickens

LU Wei, WU Yun, XU Lu, XU Qi, CHANG Guo-bin, CHEN Guo-hong^*

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2016-05-12 Online:2016-12-15 Published:2017-01-05

Abstract

Abstract: To study the SNP in TGF-β2 gene promoter and its effect on the promoter functional elements, genotyping was used by PCR-SSCP in the region of 554-824 bp(19 433 476-19 433 746 bp in Chromosome 3) of the promoter of TGF-β2 gene among 16 chicken breeds. After the direct sequencing, we found three SNP loci totally in all breeds: the C/G in 679 loci, the G/A in 699 loci and the T/C in 774 loci. Three SNPs were detected in the Big-bone chicken and Taihu chicken, but no SNP was found in AA chicken. Moreover, we analyzed the transcription factor binding sites and CpG island by JASPAR and MethPrimer software respectively in TGF-β2 gene promoter, and found that SNPs changed the transcription factor binding sites and the CpG islands. The results suggested that SNPs in TGF-β2 promoter played an important role in the regulation of gene expression through different ways.

Key words: chicken, TGF-β2, promoter, SNP

CLC Number:

S831.2

LU Wei, WU Yun, XU Lu, XU Qi, CHANG Guo-bin, CHEN Guo-hong. Polymorphism analysis of TGF-β2 gene promoter of chickens[J]. , 2016, 28(12): 2028-2032.

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Polymorphism analysis of TGF-β2 gene promoter of chickens

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