Abstract: The acute toxicity of three kinds of triazole fungicides (tebuconazole, propiconazole, difenoconazole) to zebrafish was explored, and the joint toxicity of two drugs under conditions of equivalent toxicity and equivalent concentration was explored. At the same time, a six-day embryonic exposure was also carried out to investigate the developmental effect of three triazole fungicides. The study showed that the acute toxicity of the three kinds of triazole fungicides (tebuconazole, propiconazole, difenoconazole) to embryo, larvae and adult fish of zebrafish were medium; difenoconazole and propiconazole had synergistic effects on the embryo and fish larvae of zebrafish under conditions of equivalent toxicity and equivalent concentration; the rest combination of two drugs have antagonistic effects on the embryo and larvae of zebrafish. The results of developmental toxicity test indicated that the three triazole fungicides were more toxic to zebrafish at an early life stage and could induce a series of adverse symptoms in zebrafish embryos and larvae, including decreased hatching rate, abnormal heart rate and growth inhibition, etc.

Key words: triazole fungicides, zebrafish, life stages, acute toxicity, combined toxicity, developmental toxicity