›› 2018, Vol. 30 ›› Issue (6): 926-931.DOI: 10.3969/j.issn.1004-1524.2018.06.06

• Animal Science • Previous Articles     Next Articles

Ability to break through blood-brain barrier of porcine Japanese encephalitis virus SCYA201021 strain after infection in mice

CHAI Chunxia, WANG Qiao, LAI Yalan, XU Yixuan, WEN Xintian, CAO Sanjie, HUANG Xiaobo, WEN Yiping, ZHAO Qin, WU Rui*   

  1. Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2017-10-30 Online:2018-06-20 Published:2018-07-02

Abstract: In the present study, SPF Kuming mice were inoculated with SCYA201021 strain (F122 generation, attenuated strain of porcine Japanese encephalitis virus) by intracerebral injection in order to evaluate its potential value as the attenuated live vaccine, and its ability to break through the blood-brain barrier in mice. After infection in mice, pathological changes in mice brain were observed, and the E gene copes were measured by fluorescent quantitative PCR in the blood and brain at 6, 12, 18, 24 h after inoculation, respectively. The results indicated that the virulence of SCYA201021 strain (F122 generation) was significantly weakened as the half lethal does (LD50) in suckling mice brain was only 4×105 PFU·40 μL-1. Moreover, the mice did not show pathological changes in the brain during the 24 h after intraperitoneal injection, and the quantitative PCR results were negative, indicating that the SCYA201021 strain could not break through the blood-brain barrier in mice and the SCYA201021 strain had high safety.

Key words: porcine Japanese encephalitis virus, half lethal does, fluorescent quantitative PCR, blood-brain barrier

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