Optimization of efficient regeneration system in stem of potato using response surface methodology

doi:10.3969/j.issn.1004-1524.2018.06.05

›› 2018, Vol. 30 ›› Issue (6): 918-925.DOI: 10.3969/j.issn.1004-1524.2018.06.05

• Crop Science • Previous Articles Next Articles

Optimization of efficient regeneration system in stem of potato using response surface methodology

JIANG Linjuan¹, ZOU Xue¹, HUANG Xueli¹, NI Su¹, LI Liqin¹, YANG Shimin^{1, 2, *}

1. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;
2.National Demonstration Center for Experimental Crop Science Education, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-09-04 Online:2018-06-20 Published:2018-07-02

Abstract

Abstract: To establish the optimal hormone concentrations of culture medium for efficient regeneration of potato stem callus tissue, as well as build a new system for tissue culture and rapid propagation technology, stem of Chuanyu No.10 was employed as explants to induce callus tissue, and the Box-Behnken design method of response surface methodology (RSM) was used to research the effect of TDZ, 2,4-D and GA₃ on the differentiation of potato callus. Meanwhile, the hormone concentrations were optimized. The results showed that the sensitivity of the hormone concentrations in culture medium to potato callus budding from the response surface method optimization model is TDZ>GA₃>2,4-D. The determination conefficient R² of the model was 0.992 1, the optimal medium for adventitious bud differentiation from stem callus of Chuanyu No.10 was MS+2.02 mg·L^-1 TDZ+0.08 mg·L^-1 2,4-D+2.25 mg·L^-1 GA₃.In this condition, the budding rate was actual measured, which was consistent with the predicted values. That confirmed the validity of the model.

Key words: potato, callus, response surface method, medium

CLC Number:

S532

JIANG Linjuan, ZOU Xue, HUANG Xueli, NI Su, LI Liqin, YANG Shimin. Optimization of efficient regeneration system in stem of potato using response surface methodology[J]. , 2018, 30(6): 918-925.

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Optimization of efficient regeneration system in stem of potato using response surface methodology

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