Cloning and expression analysis of StDWF1 in Solanum tuberosum

doi:10.3969/j.issn.1004-1524.2018.06.04

›› 2018, Vol. 30 ›› Issue (6): 909-917.DOI: 10.3969/j.issn.1004-1524.2018.06.04

• Crop Science • Previous Articles Next Articles

Cloning and expression analysis of StDWF1 in Solanum tuberosum

TANG Xiao¹, DENG Mengsheng¹, ZOU Xue², Li Liqin^{1, 3}, ZHU Yuanzhi^{1, 3}, WANG Xiyao^{1, 3, *}

1. Potato Research and Development Center, College of Agronomy,Sichuan Agricultural University, Chengdu 611130, China;
2. Mianyang Academy of Agricultural Sciences, Mianyang 621000, China;
3. National Demonstration Center for Experimental Crop Science Education (Sichuan Agricultural University), Chengdu 611130, China

Received:2018-01-04 Online:2018-06-20 Published:2018-07-02

Abstract

Abstract: The DWF1 is a key gene in brassinosteroid (BR) biosynthesis pathway. To explore the function of StDWF1 in the growth and development of potato, this experiment was based on the potato variety Favorita test-tube plantlets, and we cloned the full-length sequence of StDWF1 gene. The open reading frame (ORF) of StDWF1 gene was 1 704 bp, and encoded 567 amino acids. The molecular weight of protein was 66.08 ku, and the isoelectric point (pI) was 7.96. Agrobacterium tumefaciens-mediated transient expression was carried out in tobacco, and laser scanning confocal microscopy showed that potato StDWF1 was located in cytoplasm. Fluorescence quantitative analysis showed StDWF1 expression quantity was the highest in new leaves, followed by old leaves, stolon, roots, stems and tubers. We analyzed the expression of StDWF1 in potato leaves at the growth period, and the results showed that the highest expression quantity was in potato seedling stage. The results of this experiment laid the theoretical foundation for further interpreting the regulation mechanism of BR in the potato growth and development.

Key words: potato, DWF1, gene cloning, expression analysis, subcellular localization

CLC Number:

S532

TANG Xiao, DENG Mengsheng, ZOU Xue, Li Liqin, ZHU Yuanzhi, WANG Xiyao. Cloning and expression analysis of StDWF1 in Solanum tuberosum[J]. , 2018, 30(6): 909-917.

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Cloning and expression analysis of StDWF1 in Solanum tuberosum

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