Differences in soil microbial community diversity between healthy and scab-diseased potato plants in root zone

doi:10.3969/j.issn.1004-1524.20240756

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (8): 1743-1754.DOI: 10.3969/j.issn.1004-1524.20240756

• Environmental Science • Previous Articles Next Articles

Differences in soil microbial community diversity between healthy and scab-diseased potato plants in root zone

TAN Haixia¹^,²(), PENG Hongli¹^,², WANG Lianlong¹, WEI Jianmei¹

1. Department of Ecology, Hebei University of Environmental Engineering, Qinhuangdao 066102, Hebei, China
2. Hebei Key Laboratory of Agroecological Safety, Qinhuangdao 066102, Hebei, China

Received:2024-08-23 Online:2025-08-25 Published:2025-09-03

Abstract

Abstract:

To explore the differences in soil microbial diversity, soil samples were collected from the root zone of healthy potato plants (HS) and scab-diseased potato plants (OS), and high-throughput sequencing was used. The results indicated that the ACE index, Chao1 index and Shannon index of the bacterial community in OS were significantly (p<0.05) higher than those of HS, yet there was no significant difference in the alpha diversity indexes for the fungal community. At the phylum level, compared with OS, the relative abundance of Proteobacteria, Gemmatimonadota, Bacteroidota and Basidiomycota in HS was decreased significantly, while the relative abundances of Acidobacteriota, Actinobacteriota, Chloroflexi, Ascomycota and Chytridiomycota in HS was increased significantly. At the genus level, the relative abundance of Sphingomonas, Lysobacter, Tausonia, Humicola, Alternaria and Mortierella dcreased in the HS, while the relative abundances of Sonoraphlyctis and Penicillium increased. Principal co-ordinates analysis (PcoA) and LEfSe analysis indicated that there was significant difference in the soil microbial community structure between HS and OS. For soil chemical properties, the pH value and available potassium content of HS was significantly lower than that of OS, yet the content of soil organic matter, alkali-hydrolyzable nitrogen, available phosphorus in HS was significantly higher. Functional prediction results showed that there were significant differences in bacterial carbon metabolism and purine metabolism functions. The fungal community was mainly saprotroph in both HS and OS, yet the relative abundance of wood saprotroph in the OS was significantly higher than that of HS. These findings revealed the differences in the structure and function of soil microorganisms in the root zone between scab-diseased potato plants and healthy plants, which could provide theoretical basis for the effective prevention and control of potato scab disease.

Key words: potato common scab, root zone soil, microbial composition and structure, functional prediction

CLC Number:

TAN Haixia, PENG Hongli, WANG Lianlong, WEI Jianmei. Differences in soil microbial community diversity between healthy and scab-diseased potato plants in root zone[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1743-1754.

Figures/Tables 9

Fig.1 Operational taxonomic units (OTUs) of bacteria (left) and fungi (right) in potato root zone soil

Table 1 Alpha diversity indices of microbial communities in potato root zone soil

微生物类别 Microbial type	土壤样品 Soil sample	ACE指数 ACE index	Chao1指数 Chao1 index	辛普森指数 Simpson index	香农指数 Shannon index	覆盖率 Coverage/%
细菌Bacteria	HS	1 309.18±21.64 b	1 306.28±21.99 b	0.993 8±0.000 5 a	8.62±0.03 b	99.98±0.01 a
	OS	1 371.63±7.20 a	1 368.74±7.03 a	0.994 5±0.000 3 a	9.02±0.02 a	99.98±0.01 a
真菌Fungi	HS	233.82±35.89 a	233.46±36.26 a	0.803 4±0.215 2 a	4.20±1.68 a	99.99±0.01 a
	OS	294.00±40.44 a	293.58±40.65 a	0.935 2±0.011 4 a	5.19±0.33 a	99.99±0.01 a

Fig.2 Community compostion and relative abundance of main bacteria at phylum (left) and genus (right) level in potato root zone soil

Fig.3 Community compostion and relative abundance of main fungi at phylum (left) and genus (right) level in potato root zone soil

Fig.4 Principal coordinates analysis (PCoA) of bacterial (left) and fungal (right) community in potato root zone soil PCo1, Principal coordinate 1; PCo2, Principal coordinate 2.

Fig.5 Distribution of linear discriminant analysis (LDA) scores for bacterial (left) and fungal (right) community in potato root zone soil k_, p_, c_, o_, f_, g_, s_ indicate categorical level of kingdom, phylum, class, order, family, genus, species, respectively. LDA SCORE (log 10) represents the common logarithm value of LDA score.

Table 2 Chemical properties of soil in potato root zone

土壤样品 Soil sample	pH值 pH value	有机质含量 Organic matter content/ (g·kg^-1)	碱解氮含量 Alkali-hydrolyzable nitrogen content/(mg·kg^-1)	有效磷含量 Available phosphorus content/(mg·kg^-1)	速效钾含量 Available potassium content/(mg·kg^-1)
HS	4.74±0.01 b	11.51±0.01 a	96.33±0.58 a	157.17±1.05 a	308.67±0.67 b
OS	5.64±0.01 a	9.02±0.07 b	64.42±0.46 b	94.13±0.06 b	332.49±0.58 a

Fig.6 Correlation between soil chemical factors and bacterial community strucutre at phylum level (left) and fungal community structure at genus level (right) in potato root zone “*” “**” “***” represent significant correlation at p<0.05,p<0.01, p<0.001 level, respectively. AK, Available potassium content; AN, Alkali-hydrolyzable nitrogen content; OM, Organic matter content; AP, Available phosphorus content.

Fig.7 Functional prediction of bacterial (up) and fungal (down) community in potato root zone soil

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Differences in soil microbial community diversity between healthy and scab-diseased potato plants in root zone

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