Proteomic analysis of maize seedling leaves in response to heat stress

doi:10.3969/j.issn.1004-1524.2018.06.03

›› 2018, Vol. 30 ›› Issue (6): 893-908.DOI: 10.3969/j.issn.1004-1524.2018.06.03

• Crop Science • Previous Articles Next Articles

Proteomic analysis of maize seedling leaves in response to heat stress

SHI Jiang¹, ZHAO Lin¹, ZHU Yueqing², LOU Xuping³, YU Jianzhong⁴, RUAN Songlin¹, CHEN Wenyue^{1, *}

1. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. Lin'an District Forestry Bureau (Agricultural Bureau) of Hangzhou, Hangzhou 311300, China;
3. Agricultural Science and Technology Institute of Xiaoshan District of Hangzhou City, Hangzhou 311202, China;
4. Agricultural Bureau of Chun'an County of Hangzhou City, Hangzhou 311700, China;

Received:2018-02-15 Online:2018-06-20 Published:2018-07-02

Abstract

Abstract: Maize is a warm-tempered crop, but it is sensitive to high temperature weather during its growth and development, which is easy to cause the decline of yield. In this study, seedlings of a heat-resistant maize variety Xiantian No. 5 as experimental materials were treated for 0, 0.5, 3.0 h at 42 ℃, and leaf proteins from all treatments was extracted to carry out screening of differential proteins, and then the GO classification and KEGG pathway were analyzed. The results showed that 181 proteins including 95 up-regulated proteins (quantitative ratio>1.20 and P<0.05) and 86 down-regulated proteins (quantitative ratio<0.83 and P<0.05) were identified. Compared with the control, the number of up-regulated proteins increased with the increase of heat treatment time, while the number of down-regulated proteins decreased. GO classification indicated that in these differentially up-regulation proteins, there were 2 proteins enriched and designated to 2 cell components, 18 proteins enriched and designated to 8 molecular functions and 15 proteins enriched and involved in 17 biological processes. Similarly, in differentially down regulated proteins, there were 2 proteins enriched and distributed in 8 cell components, 29 proteins were enriched and designated to 11 molecular functions. 45 proteins involved in 18 biological processes. KEGG pathway analysis revealed that 16 differentially up-regulated proteins were enriched and designated to participate in 6 pathways, while 14 differentially down-regulated proteins were enriched and designated to participate in 8 pathways. Domain analysis revealed that 27 differentially up-regulated proteins were enriched and designated to 22 various domains, while 34 differentially down-regulated proteins were enriched and designated to 21 different domains. According to the searching information in NCBI database, we found that 5 proteins of Dna J molecular chaperone family including heat shock protein, thioredoxin H type, dehydrogenase and ubiquitin binding enzyme were likely to be closely related to heat resistance. In conclusion, identification of proteins related to heat stress response can provide an insight to further revealing the underlying molecular mechanism of maize response to heat stress.

Key words: maize, heat response, proteomics, GO classification, KEGG pathway analysis

CLC Number:

S513

SHI Jiang, ZHAO Lin, ZHU Yueqing, LOU Xuping, YU Jianzhong, RUAN Songlin, CHEN Wenyue. Proteomic analysis of maize seedling leaves in response to heat stress[J]. , 2018, 30(6): 893-908.

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Proteomic analysis of maize seedling leaves in response to heat stress

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