›› 2018, Vol. 30 ›› Issue (6): 886-892.DOI: 10.3969/j.issn.1004-1524.2018.06.02

• Crop Science • Previous Articles     Next Articles

Effects of combined symbiotic nitrogen-fixing bacteria on growth of wheat seed under different environmental factors

DENG Tiantian, CHEN Xi*, HOU Yumeng   

  1. Henan Institute of Engineering, Zhengzhou 451191, China
  • Received:2017-10-10 Online:2018-06-20 Published:2018-07-02

Abstract: Three species of co-symbiotic nitrogen fixation bacteria were obtained from 13 strains of wheat. Different strains were determined by the single factor batch experiments to obtain the optimal culture time, temperature, pH, glucose concentration, NaCl concentration and nitrogen concentration; furthermore, to investigate the effects of the single strain and combination groups on the growth of wheat seeds. The results showed that under the conditions of pH 7, the temperature of 35 ℃, NaCl concentration of 5%, glucose concentration of 10%, nitrogen concentration of 3 g·L-1, culture time of 36 h, the combination of group action showed better effect than the single strain, and compared with the control, the germination rate was increased by 4% on average, the germination potential, germination index also increased. To some extent, the combinatorial bacteria group can better adapt to the changeable environment, playing a stable role in promoting the yield.

Key words: symbiotic nitrogen fixing bacteria, growth curve, wheat

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