Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (9): 2023-2032.DOI: 10.3969/j.issn.1004-1524.20221279

• Crop Science • Previous Articles     Next Articles

Identification and analysis of HP gene family in wheat

LOU Yuangen1(), LI Chuang2, LI Jingjing1, XING Guozhen2, LU Yanan2, ZHENG Wenming2,*()   

  1. 1. Henan Vocational College of Nursing, Anyang 455000, Henan, China
    2. College of Life Sciences/State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2022-08-31 Online:2023-09-25 Published:2023-10-09
  • Contact: ZHENG Wenming

Abstract:

HP (histidine phosphotransfer proteins) plays an important role in regulating growth and development and responding to stress in plants. In order to understand the evolution and function of HP genes in wheat genome, this study identified the HP gene family for Triticum aestivum by the bioinformatics method, and analyzed the physicochemical properties, evolutionary characteristics, gene structure, cis-acting elements, and expression patterns under stress. The results showed that 31 HP genes were identified. The length of amid acids of HP proteins ranged from 116 to 200. Comparison with HP proteins of other plants and analysis of protein structure, gene structure and motif showed that sequences of HP gene family were conserved in wheat. Analysis of cis-acting elements revealed that the promoter regions of HP genes contained regulatory elements involved in abiotic stress, such as light, phytohormone, drought and low temperature. Heatmap analysis showed that HP genes significantly differed in the expression levels under abiotic stress. qRT-PCR analysis indicated that TaHP5-6B was strongly induced under low phosphorus stress. In conclusion, HP family genes identified at the genome-wide level are important gene resources associated with abiotic stress response in wheat.

Key words: wheat, histidine phosphotransfer protein, conserved sequence, HP gene family, low phosphorus stress

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