Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)

doi:10.3969/j.issn.1004-1524.2018.08.08

›› 2018, Vol. 30 ›› Issue (8): 1328-1335.DOI: 10.3969/j.issn.1004-1524.2018.08.08

• Animal Science • Previous Articles Next Articles

Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)

MA Xiaoping¹, YANG Qiuxia¹, YU Yan¹, LI Desheng², WANG Chengdong², LING Shanshan², GU Yu^3,*

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. China Conservation and Research Center for Giant Panda, Ya'an 625000, China;
3. College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China;

Received:2018-01-08 Online:2018-08-25 Published:2018-08-28

Abstract

Abstract: In order to explore the difference of biological characteristics and drug sensitivity of Cladosporium cladosporioides (Z20) and the mutant strain (Zt), Z20 and Zt were continuously incubated on PDA medium for 7 d, and the colony morphology and growth information were observed and recorded every day. The ultrastructure of Z20 and Zt were observed under the transmission electron microscope. The polysaccharide contents of Z20 and Zt were detected by sulfuric acid-phenol method. The minimum inhibitory concentrations (MICs) of five commonly used antifungal agents against Cladosporium cladosporioides Z20 and Zt were determined by 96-well plate microdilution method. The results showed as follows: The growth rate of Zt was higher than Z20, the colour of colony was shallow, but the number of spores decreased on PDA by continuously incubate. Under the transmission electron microscope, the mycelial wall thickness and mycelial width of wild strain Z20 were significantly greater than those of mutant strain Zt, and sugar content of mycelium was also significantly higher than that of mutant strain Zt. Antifungal susceptibility test showed that except griseofulvin, the sensitivity of mutant strain Zt to five drugs were higher than that of the Z20.

Key words: Cladosporium cladosporioides, biological characteristics, minimal inhibitory concentration, drug sensitivity test

CLC Number:

MA Xiaoping, YANG Qiuxia, YU Yan, LI Desheng, WANG Chengdong, LING Shanshan, GU Yu. Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)[J]. , 2018, 30(8): 1328-1335.

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Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)

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