Microbial ecological process of polluted urban river purified by Iris tectorum combined with immobilized bacteria

doi:10.3969/j.issn.1004-1524.2019.01.16

›› 2019, Vol. 31 ›› Issue (1): 121-129.DOI: 10.3969/j.issn.1004-1524.2019.01.16

• Environmental Science • Previous Articles Next Articles

Microbial ecological process of polluted urban river purified by Iris tectorum combined with immobilized bacteria

LIU Zongnan^1,2, WANG Xin², WU Yifei², YAO Xiaohong², SUN Hong², SHEN Qi², LI Weilin¹, TANG Jiangwu^2,*

1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-04-19 Online:2019-01-25 Published:2019-04-09

Abstract

Abstract: In order to explore the plant-microorganism interaction and coupling effects, high throughput sequencing technique was used to study the dynamic changes of microbial community in the river sewage by the coupling of Iris tectorum and immobilized water purification bacteria. The source water used in the experiment was river sewage. Four experimental groups were set up: Iris tectorum, Iris tectorum and immobilized water purification bacteria, immobilized water purification bacteria, and blank control. The dynamic simulation experiment was carried out in the experimental device by controlling the flow rate. The results demonstrated that, compared with the inflow water, the ammonia nitrogen in the outflow was on a downward trend, and remained stable after 6 days. The removal efficiency of total nitrogen was low. Total organic carbon in the water also presented a downturn trend and remained stable after 3 days. The removal efficiency of chemical oxygen demand was low. The water was rich in bacterial diversity, covering 10 phyla and 67 genera. The bacterial communities presented a dynamic changing function, which was influenced both by Iris tectorum and immobilized water purification bacteria. Ammonia oxidizing functional microorganisms were mainly affiliated in Proteobacteria, with rather low relative abundance in the species of known classifications. Denitrification functional microorganisms mainly focused on 6 genera of Proteobacteria, whose communities also presented a dynamic changing process. The results showed that Iris tectorum and immobilized bacteria had a significant effect on the water purification process, and had a certain synergistic effect on the changes of bacterial community in water body, and the effect of immobilized water purification bacteria was stronger than that of Iris tectorum.

Key words: water pollution, immobilized bacteria, high-throughput sequencing

CLC Number:

X522

LIU Zongnan, WANG Xin, WU Yifei, YAO Xiaohong, SUN Hong, SHEN Qi, LI Weilin, TANG Jiangwu. Microbial ecological process of polluted urban river purified by Iris tectorum combined with immobilized bacteria[J]. , 2019, 31(1): 121-129.

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Microbial ecological process of polluted urban river purified by Iris tectorum combined with immobilized bacteria

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