Construction of compound bacteria for polluted water purification based on mixed culture and PCR-denaturing gradient gel electrophoresis

doi:10.3969/j.issn.1004-1524.2019.11.16

›› 2019, Vol. 31 ›› Issue (11): 1896-1902.DOI: 10.3969/j.issn.1004-1524.2019.11.16

• Environmental Science • Previous Articles Next Articles

Construction of compound bacteria for polluted water purification based on mixed culture and PCR-denaturing gradient gel electrophoresis

WANG Xin, TANG Jiangwu^*, WU Yifei, YAO Xiaohong, SUN Hong, SHEN Qi, LI Yuancheng

Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-07-22 Online:2019-11-25 Published:2019-12-04

Abstract

Abstract: In the current study, 11 strains with different water purification functions were chose to be cultured together in LB medium, and PCR-denaturing gradient gel electrophoresis (DGGE) was used to reveal the changes of bacterial community after co-culturing to determine the coexistence and growth characteristics of these strains. The dominant strains in mixed culture were used as the core to form different compound bacteria, and their functional effects were determined through water purification experiments. The results showed that strains AOZ1 or BSK9, W14 or BSK3 could grow effectively under mixed culture conditions, and were the dominant bacteria in mixed culture. With strains AOZ1 or BSK9 as the core strains, the compound water purification functional bacteria could be constructed. Among them, the compound bacteria composed of strain AOZ1, BSK9, W14 and BSK3 had high removal rates of chemical oxygen demand (COD) and ammonia nitrogen, which reached 48.0% and 76.8%, respectively. The results suggested that compound water purification bacteria could be effectively constructed by the liquid co-culture method along with PCR-DGGE.

Key words: compound bacteria, microbial enhanced remediation, water pollution control

CLC Number:

WANG Xin, TANG Jiangwu, WU Yifei, YAO Xiaohong, SUN Hong, SHEN Qi, LI Yuancheng. Construction of compound bacteria for polluted water purification based on mixed culture and PCR-denaturing gradient gel electrophoresis[J]. , 2019, 31(11): 1896-1902.

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Construction of compound bacteria for polluted water purification based on mixed culture and PCR-denaturing gradient gel electrophoresis

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