›› 2019, Vol. 31 ›› Issue (5): 704-708.DOI: 10.3969/j.issn.1004-1524.2019.05.04

• Animal Science • Previous Articles     Next Articles

Molecular cloning and bioinformatics analysis of white king pigeon (Columba livia) ovalbumin-related protein Y gene

XU Xiaoqin1,2,3, DU Xue1, LI Guoqin1,4, ZHANG Long2, TAO Zhengrong1, LU Lizhi1,4,*   

  1. 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. Institute of Ecology, China West Normal University, Nanchong 637000, China;
    3. College of Animal Sciences & Technology, Nanjing Agricultural University, Nanjing 210095, China;;
    4.Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs, Hangzhou 310021,China
  • Received:2018-09-28 Online:2019-05-25 Published:2019-05-23

Abstract: The experiment was conducted to clone the whole cDNA of OVALY gene in Columba liviaand analyze the bioinformatic information of the cDNA sequence.The nucleotide sequence of a full-length 2 068 bp cDNA for OVALY was obtained, containing 5'UTR (180 bp), CDS (1 164 bp) and 3'UTR (720 bp), and coding 388 amino acids (44.19 ku, GenBank accession number: KX230792). The OVALY cDNA sequence of white king pigeon was most similar to Nipponianippon, and the homology was 81%. This sequence was also similar to Haliaeetusalbicilla and Pteroclesgutturalis, and the homologies were 80% and 79%, respectively. 4 N-glycosylated sites and 22 phosphorylation sites were detected in these amino acids. Conserved domain analysis of OVALY protein showed this protein had a conserved domain for serpin superfamily.

Key words: Columba livia, OVALY, RACE

CLC Number: