Isolation, identification and phylogenetic analysis on gp85 gene of subgroup J avian leukosis virus

doi:10.3969/j.issn.1004-1524.2019.05.05

›› 2019, Vol. 31 ›› Issue (5): 709-715.DOI: 10.3969/j.issn.1004-1524.2019.05.05

• Animal Science • Previous Articles Next Articles

Isolation, identification and phylogenetic analysis on gp85 gene of subgroup J avian leukosis virus

WANG Yuanhong¹, LU Zhimin¹, ZHANG Xueqi¹, LIU Zimin¹, HU Zihui¹, YANG Kankan¹, LIU Hongmei¹, PAN Ling¹, PENG Kaisong¹, LI Yongdong^2,*, WANG Yong^1,*

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2. Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China

Received:2018-09-19 Online:2019-05-25 Published:2019-05-23

Abstract

Abstract: To identify the avian leukosis virus (ALV) in chicken farm in Chaohu, Anhui Province, the virus was isolated from the tumor tissue by using DF-1 cells. The results were confirmed by RT-PCR and transmission electron microscopy. The diseased chicken organs such as liver, heart, spleen and glandular stomach were analyzed by clinical observation, pathological anatomy and histopathological examination. Finally, the gene of envelope protein gp85 were cloned and sequenced by designing specific primers for gp85 motif from ALV reference strains in GenBank, and their nucleotide homologies were compared. The results showed that a virus strain, AHCH-2018, was confirmed by sequencing the 928 bp PCR amplification product. The virus about 100 nm in diameter appeared under the transmission electron microscope, which was enveloped and spherical in shape. Histopathological observation showed that larger areas of the pericardial fat, myocardium, spleen, liver and glandular stomach were infiltrated by lymphocytes and lymphoid cells. The gp85 gene nucleotide sequence analysis showed that the identity of AHCH-2018 strain and other ALV J reference strains ranged from 94.1%-99.5%, amongst which the highest identity (99.5%) was shown with GD1401J strain, when compared with ALV A, B, C, D and E, the nucleotide identity ranged from 46.5%-49.7%. The phylogenetic analysis showed that AHCH-2018 and J subgroup prototype strain were present in the same branch. This study provided a data reference for understanding the molecular epidemiological characteristics of avian leukemia in Anhui Province, China.

Key words: Avian leukosis, histopathology, gp85 gene, sequence analysis

CLC Number:

S858.31

WANG Yuanhong, LU Zhimin, ZHANG Xueqi, LIU Zimin, HU Zihui, YANG Kankan, LIU Hongmei, PAN Ling, PENG Kaisong, LI Yongdong, WANG Yong. Isolation, identification and phylogenetic analysis on gp85 gene of subgroup J avian leukosis virus[J]. , 2019, 31(5): 709-715.

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Isolation, identification and phylogenetic analysis on gp85 gene of subgroup J avian leukosis virus

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