Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum

doi:10.3969/j.issn.1004-1524.2017.02.02

›› 2017, Vol. 29 ›› Issue (2): 186-192.DOI: 10.3969/j.issn.1004-1524.2017.02.02

• Crop Science • Previous Articles Next Articles

Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum

WANG Yan¹, LIU Yao², LIANG Chenggang^{1, *}, CHEN Qingqing¹, SHI Taoxiong¹, CHEN Qijiao¹, MENG Ziye¹, CHEN Qingfu¹

1. Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China;
2. Shaanxi Micangshan National Nature Reserve, Hanzhong 723500, China

Received:2016-07-29 Online:2017-02-15 Published:2017-03-06

Abstract

Abstract: ABA-insensitive (ABI) is a kind of transcription factors that regulates plant stress response, seed shattering and dormancy. Bioinformatic analysis indicated that the cDNA sequence of common buckwheat ABI(CL38501) gene contained 963 bp, coded 320 amino acids including the bZIP domain and the conserved domain Ⅱ of ABI5, suggesting that it might encode the ABI5 family protein. The major genes involve in ABA signal transduction pathway were up-regulated in cultivar including ABI that was up-regulated by 4.42-fold and 3.66-fold at bud stage and flowering stage, respectively, as compared with the wild germplasm of common buckwheat. A total of 405 sites in ABI gene including 30 polymorphic sites were identified among 9 cultivars and 5 wild germplasms of common buckwheat. Only 1 polymorphic site was identified among 9 cultivars, whereas 7 polymorphic sites were identified in 5 wild germplasms. These results indicated that the ABI sequence of common buckwheat was highly conserved. A total of 11 consistent polymorphic amino acid sites were identified among cultivar and wild germplasm. The change of protein conformation was found between cultivar and wild germplasm of common buckwheat induced by the amino acid transformation at the 32nd and 60th sites of ABI, implying that it might be associated with the loss of seed shattering and dormancy during the long domestication process.

Key words: common buckwheat, ABA-insensitive gene, sequence analysis, protein structure

CLC Number:

WANG Yan, LIU Yao, LIANG Chenggang, CHEN Qingqing, SHI Taoxiong, CHEN Qijiao, MENG Ziye, CHEN Qingfu. Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum[J]. , 2017, 29(2): 186-192.

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Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum

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