Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep

doi:10.3969/j.issn.1004-1524.2019.09.03

›› 2019, Vol. 31 ›› Issue (9): 1416-1422.DOI: 10.3969/j.issn.1004-1524.2019.09.03

• Animal Science • Previous Articles Next Articles

Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep

BAI Junyan, CAO Heng, WANG Xu, YANG Youbing, FAN Hongdeng, FU Xueyan, SHI Kunpeng, DONG Zhihao, LU Xiaoning, LI Xinyue, HAO Weiguang, LI Ziheng, ZHENG Feiyang

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China

Received:2019-03-11 Online:2019-09-25 Published:2019-10-11

Abstract

Abstract: To investigate the relationship between GH gene and growth and development performance of sheep, the polymorphism of PvuⅡ locus in intron Ⅱ of GH gene in five sheep populations was analyzed by PCR-RFLP, and the association between the polymorphism of PvuⅡ locus and sheep weight and body size was analyzed. The results showed that two genotypes, AB genotype (264 bp/429 bp/693 bp) and BB genotype (264 bp/429 bp), were detected at PvuⅡ locus of intron Ⅱ of GH gene in five sheep. The AB genotype frequencies of Large-tailed Han sheep, Small-tailed Han sheep, Yuxi fat-tailed sheep, Hu sheep and Dorper sheep were 0.786, 0.750, 0.424, 0.471 and 0.459 respectively, and BB genotype frequencies were 0.214, 0.250, 0.576, 0.529 and 0.541, respectively. The PvuⅡ locus of GH gene intron Ⅱ in Hu sheep deviated significantly from Hardy-Weinbery equilibrium (P<0.05), and the PvuⅡ locus of GH gene intron Ⅱ in Small-tailed han sheep, Yuxi fat-tailed sheep and Dupo sheep deviated significantly from Hardy-Weinbery equilibrium (P<0.01). The correlation analysis showed that the body weight, body length, chest width, buttock height, cannon circumference, rump height and neck length of AB genotype sheep were significantly higher than those of BB genotype sheep (P<0.05). The results showed that the PvuⅡ locus of intron Ⅱ of GH gene had some effects on the growth and development performance of sheep.

Key words: sheep, GH gene, PCR-RFLP, association analysis

CLC Number:

S826

BAI Junyan, CAO Heng, WANG Xu, YANG Youbing, FAN Hongdeng, FU Xueyan, SHI Kunpeng, DONG Zhihao, LU Xiaoning, LI Xinyue, HAO Weiguang, LI Ziheng, ZHENG Feiyang. Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep[J]. , 2019, 31(9): 1416-1422.

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Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep

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