›› 2019, Vol. 31 ›› Issue (9): 1423-1428.DOI: 10.3969/j.issn.1004-1524.2019.09.04

• Animal Science • Previous Articles     Next Articles

Epidemiological investigation of porcine epidemic diarrhea virus and sequence analysis of ORF3 and S1 genes in Sichuan from 2016 to 2018

DEXI Cuomu1, WANG Yin2,*, YANG Zexiao2, YAO Xueping2, LUO Yan2, LIAO Changyu2, ZHANG Pengfei2, JIANG Dike2, XIANG Mingyuan2, JIANG Ruijiao2, SONG Yong3   

  1. 1. Zuogong County Animal Husbandry Station, Changdu 854499, China;
    2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    3. Ruipu Biological Co., Ltd., Tianjin 300300, China
  • Received:2018-07-15 Online:2019-09-25 Published:2019-10-11

Abstract: To analyze the prevalence and molecular genetic evolution of pig epidemic diarrhea in Sichuan, this study conducted RT-PCR and related epidemiological investigations on 71 cases of diarrhea collected from June 2016 to June 2018, and ORF3 and S1 genes of four positive samples were tested and sequenced. The results showed that the peak incidence days in the past two years were January 28 and February 11, respectively, and the peak incidence period was from December to March. The number of infections in pig farms decreased year by year, and the new epidemic source increased. ORF3 gene can be divided into two genotypes, some strains had a small number of base mutations. The results of S1 gene high-variation segmentation showed that the prevalent PEDV strain from 2016 to 2018 was the GII group, which was the same as the current popular PEDV variant strain. The homology was 92.8%~99.3% and the number of base variation was large. The relationship with the domestic classic vaccine strain CV777 was far and the homology was 82.7%~87.3%. The incidence period was more concentrated than the traditional PEDV. The results showed that the incidence of PED in large-scale pig farms in Sichuan showed a downward trend, and preliminary results were obtained for the prevention and control of the disease. The current trend of PEDV strains was mutated. This study could lay the foundation for the prevention and control of PEDV.

Key words: porcine epidemic diarrhea virus, epidemiological survey, gene sequence analysis

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