Simultaneous detection and analysis of 16 kinds of mycotoxins in Pu-erh tea

doi:10.3969/j.issn.1004-1524.2019.10.16

›› 2019, Vol. 31 ›› Issue (10): 1700-1708.DOI: 10.3969/j.issn.1004-1524.2019.10.16

• Food Science • Previous Articles Next Articles

Simultaneous detection and analysis of 16 kinds of mycotoxins in Pu-erh tea

HU Lin¹, SHI Zhen², ZHAO Li², LIU Xiaosong², DONG Yuying², JIANG Mengyuan^1,*, LI Wenting^2,*

1. Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, China;
2. Kunming Center for Disease Control and Prevention, Kunming 650228, China

Received:2019-03-27 Online:2019-10-25 Published:2019-10-30

Abstract

Abstract: To establish a convenient and accurate method for the determination of 16 mycotoxins with liquid chromatography-tandem mass spectrometry(LC-MS/MS) in Yunnan Pu-erh tea, including aflatoxin, fumonisin (FB₁-FB₂), nivalenol, deoxynivalenol (DON), zearalenone, ochratoxin A, T-2 toxin, HT-2 toxin and heterochromatoxin, samples were extracted by organic solvent (methanol-water-formic acid volume ratio was 70∶29∶1), purified by PriboFast^® Multi-Toxin IAC immunoaffinity purification column, determined and analyzed by multi-reaction monitoring of LC-MS/MS, and quantified by internal standard method. The regression equations of 16 mycotoxins had a good linear relationship(r>0.99). The recovery rate at three different levels was 84.2%-102.7%, and the relative standard deviation was 2.18%-4.78%. In the detection of 16 kinds of mycotoxins in 174 samples of Yunnan Pu'er tea, DON was detected in 53 samples of tea with the detection rate of 30.63%, FB₁ was detected in 96 samples of tea with the detection rate of 55.17%, and FB₂ was detected in 12 samples of tea with the detection rate of 6.90%, FB₃ was detected in 12 samples of tea with the detection rate of 6.90%. Other mycotoxins were not detected. The contents of the 4 mycotoxins detected were between 0.2-6.0 μg·kg^-1, which did not exceed the international food safety limit. The detection rate of mycotoxins in Pu-erh tea in Yunnan Province was low, and the content of mycotoxins met the requirements of national standards. The method was accurate, reliable and convenient, which can be applied to the detection of mycotoxins in tea, especially in batch tea.

Key words: mycotoxin, Pu-erh tea, liquid chromatography-tandem mass spectrometry(LC-MS/MS)

CLC Number:

TS207.4

HU Lin, SHI Zhen, ZHAO Li, LIU Xiaosong, DONG Yuying, JIANG Mengyuan, LI Wenting. Simultaneous detection and analysis of 16 kinds of mycotoxins in Pu-erh tea[J]. , 2019, 31(10): 1700-1708.

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Simultaneous detection and analysis of 16 kinds of mycotoxins in Pu-erh tea

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