Cloning, bioinformatics analysis and tissue expression detection of MCUR1 gene in Saba pig

doi:10.3969/j.issn.1004-1524.2019.11.08

›› 2019, Vol. 31 ›› Issue (11): 1825-1833.DOI: 10.3969/j.issn.1004-1524.2019.11.08

• Animal Science • Previous Articles Next Articles

Cloning, bioinformatics analysis and tissue expression detection of MCUR1 gene in Saba pig

DONG Xinxing, LI Mingli, CUI Yijia, LAN Guoxiang, WANG Xiaoyi, YAN Dawei^*

College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received:2019-04-08 Online:2019-11-25 Published:2019-12-04

Abstract

Abstract: The aim of this study was to clone the MCUR1 gene from Saba pigs, analyze it by bioinformatics, and detect its expression level in different tissues of Saba pig and Large White pig by real-time fluorescent quantitative PCR. According to the MCUR1 gene sequence published in GenBank (accession number: XM_003128183.4), the primers were designed, and the MCUR1 gene CDS sequence of Saba pig was obtained by PCR amplification and sequencing. The sequence was 1 095 bp in length and encoded 364 amino acids. The molecular formula of MCUR1 protein is C₁₇₈₇H₂₉₄₉N₅₃₃O₅₀₃S₁₄. The molecular weight is 40.398 ku, the theoretical isoelectric point (pI) is 10.27, the instability coefficient is 55.70, the fat index is 95.99, and the average hydrophilicity is -0.213, which belongs to hydrophilic protein. MCUR1 protein has no signal peptide and transmembrane structure, and contains two spiral coiled structure that function primarily in the cytoplasm; contains 32 phosphorylation sites; contains one CpG island; the secondary structure consists of 58.24% alpha-helix, 4.12% beta turn, 30.22% random coil and 7.42% extended chain. The homology of the coding region of the porcine MCUR1 gene with bovine, chicken, dog, goat, human, monkey, mouse, and sheep was 85.3%, 68.0%, 83.6%, 87.9%, 84.4%, 80.3%, 76.8% and 88.2%, respectively, showing close relationship with sheep, goat and cattle, and distant relationship with chicken. The highest expression level of MCUR1 was observed in the liver and the least expression was in the lung. The expression of MCUR1 in the longissimus dorsi of the Large White pig was higher than that of the longissimus dorsi of the Saba pig (P<0.01).

Key words: Saba pig, MCUR1 gene, bioinformatics analysis, tissue expression

CLC Number:

S828

DONG Xinxing, LI Mingli, CUI Yijia, LAN Guoxiang, WANG Xiaoyi, YAN Dawei. Cloning, bioinformatics analysis and tissue expression detection of MCUR1 gene in Saba pig[J]. , 2019, 31(11): 1825-1833.

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Cloning, bioinformatics analysis and tissue expression detection of MCUR1 gene in Saba pig

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