MicroRNA sequencing and analysis of porcine lung of Jiangquhai pig response to Mycoplasma hyopneumoniae infection

doi:10.3969/j.issn.1004-1524.2019.12.05

›› 2019, Vol. 31 ›› Issue (12): 1979-1986.DOI: 10.3969/j.issn.1004-1524.2019.12.05

• Animal Science • Previous Articles Next Articles

MicroRNA sequencing and analysis of porcine lung of Jiangquhai pig response to Mycoplasma hyopneumoniae infection

NI Ligang^1,2, ZHAO Xuting², WANG Xiaoyan^1,*, SONG Chengyi¹, WU Xinsheng¹, GAN Yuan³

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
3. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210004, China

Received:2019-04-23 Online:2019-12-25 Published:2019-12-25

Abstract

Abstract: The purpose of the study was to explore the expression profile and mechanism of miRNA of porcine lung response to Mycoplasma hyopneumoniae infection. In this experiment, Jiangquhai pigs were divided into the infected group and the control group, and the infected group was inoculated with Mycoplasma hyopneumoniae. At the 28th day after inoculation, all pigs were slaughtered and lung tissues were collected for high-throughput microRNA sequencing. The differentially expressed miRNAs were screened and the related target genes were predicted using several prediction softwares. The results showed that a total of 14 265 786 and 14 000 588 clean reads were obtained from the infected group and the control group respectively. Compared with the control group, 73 significant differentially expressed miRNAs were obtained from infected group, 39 and 34 miRNAs were up-regulated and down-regulated. Among them, 4 miRNAs were randomly selected and verified by qRT-PCR, the expression pattern were consistent with the sequencing results. 1 685 target genes and 4 220 target sites for all differentially expressed miRNAs were predicted. 8 miRNAs were related to immunoregulation. The predicted target genes were analyzed by GO, which showed that these miRNA were widely involved in the regulation of biological processes, cellular components and molecular functions cells. KEGG pathway analysis showed that the miRNAs were involved in the regulation of apoptosis, ECM-receptor interaction, focal adhesion and other signaling pathways. In this study, we obtained the miRNA expression profile of porcine lung of Jiangquhai pig response to Mycoplasma hyopneumoniae infection, and immunity-associated miRNAs and signal pathways were obtained by bioinformatics analysis, the results were helpful for understanding the mechanism of Mycoplasma hyopneumoniae infection in Jiangquhai pigs.

Key words: Jiangquhai pig, Mycoplasma hyopneumoniae, high throughput sequencing, miRNA expression profile

CLC Number:

S852.62

NI Ligang, ZHAO Xuting, WANG Xiaoyan, SONG Chengyi, WU Xinsheng, GAN Yuan. MicroRNA sequencing and analysis of porcine lung of Jiangquhai pig response to Mycoplasma hyopneumoniae infection[J]. , 2019, 31(12): 1979-1986.

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MicroRNA sequencing and analysis of porcine lung of Jiangquhai pig response to Mycoplasma hyopneumoniae infection

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