Evaluation of heat tolerance and response of enzymatic antioxidant system to heat stress in Brassica parachinensis L.

doi:10.3969/j.issn.1004-1524.2020.01.09

›› 2020, Vol. 32 ›› Issue (1): 72-79.DOI: 10.3969/j.issn.1004-1524.2020.01.09

• Horticultural Science • Previous Articles Next Articles

Evaluation of heat tolerance and response of enzymatic antioxidant system to heat stress in Brassica parachinensis L.

PANG Qiangqiang, ZHOU Man, SUN Xiaodong, ZHANG Wen, CAI Xinglai^*

Vegetable Research Institute for Hainan Provincial Academy of Agricultural Sciences, Hainan Provincial Key Laboratory for Vegetable and Biology, Hainan Provincial Engineering Research Center for Melon and Vegetable Breeding, Haikou 571100, China

Received:2019-05-09 Online:2020-01-25 Published:2020-03-11

Abstract

Abstract: In order to identify the heat tolerance of different varieties of Brassica parachinensis L., heat damage index was used as an evaluation index to evaluate the heat tolerance of 19 materials. On this basis, the response of enzymatic antioxidant system to simulated high temperature stress was studied. The results showed that through the analysis of heat damage index, 5 heat-resistant varieties, 11 medium heat-resistant varieties and 3 heat-sensitive varieties were screened out. The heat-resistant variety CX10, medium-heat-resistant variety CX17 and heat-sensitive variety CX13 under high temperature treatment showed that conductivity, MDA contents, activities of SOD, POD and CAT, expression levels of the related genes Mn-SOD, POD and CAT were increased compared with the control. The increase of conductivity and MDA content in heat-resistant varieties was significantly lower than that in heat-sensitive varieties. SOD, POD and CAT activity, as well as the expression of Mn-SOD, POD and CAT genes in the heat-resistant varieties, increased largest. After 3 days' recovery, compared with the treatment under stress, all indicators showed a downward trend, but higher than the control. These results indicated that high temperature stress affected the normal metabolism of enzymatic antioxidant system in Brassica parachinensis L. Even after high temperature was relieved, it could not return to the normal state. However, heat-resistant varieties could alleviate the damage caused by stress by regulating their protective system.

Key words: Brassica parachinensis L., heat tolerance, enzymatic antioxidant system, heat stress

CLC Number:

S634.5

PANG Qiangqiang, ZHOU Man, SUN Xiaodong, ZHANG Wen, CAI Xinglai. Evaluation of heat tolerance and response of enzymatic antioxidant system to heat stress in Brassica parachinensis L.[J]. , 2020, 32(1): 72-79.

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Evaluation of heat tolerance and response of enzymatic antioxidant system to heat stress in Brassica parachinensis L.

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