Expression and distribution of KISS-1/GPR54 on hypothalamic-pituitary-ovary axis in estrous cycle of Ganjia Tibetan sheep

doi:10.3969/j.issn.1004-1524.2020.06.04

›› 2020, Vol. 32 ›› Issue (6): 971-977.DOI: 10.3969/j.issn.1004-1524.2020.06.04

• Animal Science • Previous Articles Next Articles

Expression and distribution of KISS-1/GPR54 on hypothalamic-pituitary-ovary axis in estrous cycle of Ganjia Tibetan sheep

CHEN Weigang¹, BAO Yingying¹, HE Yuqin^1,*, GE Wenbo¹, ZHANG Xia¹, HUADAO Cairang², YANG Dapeng¹, ZHOU Kairen¹

1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Ganjia Township Animal Husbandry and Veterinary Workstation of Xiahe County in Gannan Tibetan Autonomous Prefecture, Xiahe 747199, China

Received:2019-12-04 Online:2020-06-25 Published:2020-06-24

Abstract

Abstract: The aim of this study was to clarify the expression of KISS-1/GPR54 on the hypothalamus-pituitary-ovary axis (HPOA) of Ganjia Tibetan sheep during estrus cycle and its regulation function on seasonal estrus cycle in Ganjia Tibetan sheep. In this study, 32 Ganjia Tibetan sheep in the estrus cycle were selected and the differences in the expressions and distribution of KISS-1/GPR54 on the hypothalamic-pituitary-ovary axis in the estrus cycle of Ganjia Tibetan sheep were detected by qRT-PCR and immunohistochemistry method. The qRT-PCR results showed that the expression of KISS-1 and GPR54 were found in HPOA tissues of Ganjia Tibetan sheep during the whole estrus cycle. In the hypothalamus, the relative expression levels of KISS-1 in proestrus were significantly (P<0.05) higher than the other three estrus stages. The relative expression levels of proestrus and interestrus in pituitary tissues were significantly (P<0.05) higher than that in metestrus. In the ovary axis tissue, the expression levels in the diestrus stage were significantly (P<0.05) higher than those in the other three estrus stages. The relative expression levels of GPR54 in the hypothalamus were the highest in the estrus stage, and were significantly (P<0.05) higher than those in the other three estrus stages;in the pituitary, the relative expression levels in the proestrus were significantly (P<0.05) higher than the other three estrus stages. The relative expression levels in the ovary axis tissues were the highest in proestrus, and were significantly (P<0.05) higher than that in other stages. Immunohistochemical staining results showed that kisspeptin and GPR54 positive products were mainly distributed in the cytoplasm and expressed in the arachnoid nucleus and the anterior ventral region of the ventricle in the hypothalamus. It was mainly expressed in basophil and chromophobe cells in adenohypophysis and in follicular granule layer and follicular intima in ovary axis. In conclusion, the differential expression of KISS-1 and GPR54 in the HPOA of Ganjia Tibetan sheep's estrus cycle had a certain regulatory effect on the seasonal estrus cycle of Tibetan sheep.

Key words: Ganjia Tibetan sheep, KISS-1, GPR54, estrus cycle, hypothalamic-pituitary-ovary axis (HPOA)

CLC Number:

S826

CHEN Weigang, BAO Yingying, HE Yuqin, GE Wenbo, ZHANG Xia, HUADAO Cairang, YANG Dapeng, ZHOU Kairen. Expression and distribution of KISS-1/GPR54 on hypothalamic-pituitary-ovary axis in estrous cycle of Ganjia Tibetan sheep[J]. , 2020, 32(6): 971-977.

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Expression and distribution of KISS-1/GPR54 on hypothalamic-pituitary-ovary axis in estrous cycle of Ganjia Tibetan sheep

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