Cloning and tissue expression analysis of PDK4 gene in Tunchang pig

doi:10.3969/j.issn.1004-1524.2020.06.05

›› 2020, Vol. 32 ›› Issue (6): 978-985.DOI: 10.3969/j.issn.1004-1524.2020.06.05

• Animal Science • Previous Articles Next Articles

Cloning and tissue expression analysis of PDK4 gene in Tunchang pig

SUN Ruiping^1,2, WANG Feng¹, CHAO Zhe^1,2, LIU Hailong^1,2, XING Manping¹, LIU Quanwei^1,2, HUANG Lili¹, ZHENG Xinli¹, WEI Limin^1,2,*

1. Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Science, Haikou 571100, China;
2. Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Haikou 571100, China

Received:2019-12-12 Online:2020-06-25 Published:2020-06-24

Abstract

Abstract: To obtain the coding sequence (CDS) of PDK4 gene in Tunchang pigs, analyze its molecular structure characteristics, study the expression levels of PDK4 mRNA in different tissues of Tunchang pigs, Duroc pigs and their hybrid F1 generation, pig PDK4 gene (Genbank No.: NM_001159306) was used as the reference primer, and the PDK4 CDS region of Tunchang pigs was obtained by RT-PCR amplification, sequencing and splicing. The results showed that the coding region of PDK4 gene was 1 224 bp and its relative molecular weight was 46 170.24 u. There was neither transmembrane structure nor signal peptide in PDK4 protein, and it was a non-secretory protein which mainly played a role in mitochondria. It was predicted that there could be two potential glycation sites and thirty-three phosphorylation sites, and the helical region occupied the largest proportion in the predicted secondary structure. The results of fluorescence quantitative PCR showed that in different tissues of Tunchang pig, the expression level of PDK4 gene was the highest in the longest dorsal muscle. The expression level of PDK4 gene in Tunchang pig's longest dorsal muscle was significantly higher than Duroc pig and its hybrid F1 generation.

Key words: Tunchang pig, PDK gene, cloning, tissue expression, Duroc pigs, real-time fluorescent quantitative PCR

CLC Number:

S828

SUN Ruiping, WANG Feng, CHAO Zhe, LIU Hailong, XING Manping, LIU Quanwei, HUANG Lili, ZHENG Xinli, WEI Limin. Cloning and tissue expression analysis of PDK4 gene in Tunchang pig[J]. , 2020, 32(6): 978-985.

References

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Cloning and tissue expression analysis of PDK4 gene in Tunchang pig

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