Water purification effect of Ceratophyllum demersum L. and change of microbial community under different treatments

doi:10.3969/j.issn.1004-1524.2020.06.15

›› 2020, Vol. 32 ›› Issue (6): 1070-1081.DOI: 10.3969/j.issn.1004-1524.2020.06.15

• Environmental Science • Previous Articles Next Articles

Water purification effect of Ceratophyllum demersum L. and change of microbial community under different treatments

SHI Chuanqi, HU Baozhong, YU Shaopeng^*, MENG Bo, YANG Chunxue, LIU Jia, DING Junnan

Heilongjiang Province Key Laboratory of Cold Region Wetland Ecology and Environment Research, Harbin Institution of Wetland Research, Harbin University, Harbin 150086, China

Received:2019-12-27 Online:2020-06-25 Published:2020-06-24

Abstract

Abstract: In order to study the purification effect of submerged macrophyte Ceratophyllum demersum on the still water in cold region and its microbial community change under different planting diversity and different aeration treatments, the still water samples in cold region were collected in this experiment. Aeration treatments were operated in fixed-capacity containers with C. demersum in laboratory, and the removal effects of total nitrogen (TN) and total phosphorus (TP) were determined. The high-throughput sequencing technology was used to analyze the microbial community structure and diversity change in different sampling time and treatments. Combined with the physicochemical factors of water samples, the influencing factors of microbial community change were analyzed. The results showed that: considering the C. demersum amount and energy consumption, water purification achieved better effect when the planting density of C. demersum was 4.44 g·L^-1 under the condition of 12 h aeration treatment, the removal rate of TN and TP was 51.23% and 89.27%, respectively. A total of 3 283 operational taxonomic units (OTU) and 107 core OTUs were obtained from the sequencing of microbial samples. Overall, the diversity of microbial community in each group did not increase, and the difference between groups decreased gradually. The dominant phyla were Proteobacteria, Bacteroidetes and Actinobacteria. The relative abundance of the dominant phylum could be increased by adding proper C. demersum, but the relative abundance of Proteobacteria and Bacteroidetes could be reduced by aeration treatment. There were a large proportion of unclassified groups in different samples, and Polynucleobacter in Proteobacteria had a high relative abundance. Adding proper C. demersum and aeration treatment could improve its relative abundance. Compared with the effect of TN and TP concentration changes, adding C. demersum and aeration treatment had more significant effect on microbial dominant groups.

Key words: Ceratophyllum demersum, aeration, total nitrogen, total phosphorus, microbial community

CLC Number:

SHI Chuanqi, HU Baozhong, YU Shaopeng, MENG Bo, YANG Chunxue, LIU Jia, DING Junnan. Water purification effect of Ceratophyllum demersum L. and change of microbial community under different treatments[J]. , 2020, 32(6): 1070-1081.

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Water purification effect of Ceratophyllum demersum L. and change of microbial community under different treatments

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