Improving microbial system of continuous cropping soil by addition of Trichoderma asperellum and ultrafine powder humus

doi:10.3969/j.issn.1004-1524.2020.06.14

›› 2020, Vol. 32 ›› Issue (6): 1060-1069.DOI: 10.3969/j.issn.1004-1524.2020.06.14

• Environmental Science • Previous Articles Next Articles

Improving microbial system of continuous cropping soil by addition of Trichoderma asperellum and ultrafine powder humus

LIN Hui¹, ZHANG Jin¹, YUAN Qianyu^1,2, YE Jing¹, SUN Wanchun¹, YU Yijun³, YU Qiaogang¹, MA Junwei^1,*

1. Institute of Environment, Resource, Soil & Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China;
3. Hangzhou Center for Inspection and Testing for Quality and Safety of Fertilizer, Zhejiang Cultivated Land Quality and Fertilizer Management Station, Hangzhou 310020, China

Received:2019-12-10 Online:2020-06-25 Published:2020-06-24

Abstract

Abstract: The present work focused on the effects of application of Trichoderma asperellum T-1, ultrafine powder humus and their combination (Humus-T1) on the microbial system of continuous cropping soil, to offer highly efficient, low-cost and environment friendly methods for controlling soil-borne diseases and alleviation of continuous cropping obstacles. The inhibitory effects of T. asperellum T-1 on different types of Fusarium oxysporum(F. oxysporum f. sp. cucumberium,FOC; F. oxysporum f. sp. niveum,FON; F. oxysporum f. sp. vasinfectum,FOV) were determined by plate confrontation culture experiment. Soil microcosm experiments were performed with 4 treatments, including control (CK-S), and addition of 1% (mass fraction) ultrafine powder humus (TS1), or 0.5% (mass fraction) T. asperellum T-1 (TS2) or 0.5% (mass fraction) Humus-T1 (TS3). The design of treatments CK-S, TS1 and TS3 were also applied in the cucumber pot experiment, and were marked as CK-C, TC1 and TC2, respectively. Soil samples were collected and then subjected for plate count technique and real-time quantitative polymerase chain reaction (qPCR). The population of F. xysporum, fungi, bacteria and T. asperellum was determined as well as the incidence of cucumber wilt. It was shown that T. asperellum T-1 exhibited inhibitory effects on different kinds of F. oxysporum, and the highest inhibition effect was observed on FON. Under soil microcosm condition, treatments of TS1, TS2 and TS3 inhibited the proliferation of F. oxysporum in continuous cropping soil without significantly influencing the soil microbial population. The maximum inhibition rate was found in TS3 treatment. Soil microcosm experimental results showed that the combination of ultrafine powder humus and T-1 increased the colonization of T-1 in soils. Compared with CK-C, TC2 significantly (P<0.05) increased the number of T. asperellum and Actinomycetes in soils. Besides, TC2 exhibited more persistent inhibition effect on F. oxysporum and lower mortality rate of cucumber than TC1. In conclusion, application of T. asperellum T-1, ultrafine powder humus or Humus-T1 could improve the soil microbial system, inhibite the growth of F. oxysporum in soil, and Humus-T1 showed the best result.

Key words: Trichoderma asperellum, humus, Fusarium oxysporum, soil microorganism, cucumber

CLC Number:

LIN Hui, ZHANG Jin, YUAN Qianyu, YE Jing, SUN Wanchun, YU Yijun, YU Qiaogang, MA Junwei. Improving microbial system of continuous cropping soil by addition of Trichoderma asperellum and ultrafine powder humus[J]. , 2020, 32(6): 1060-1069.

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Improving microbial system of continuous cropping soil by addition of Trichoderma asperellum and ultrafine powder humus

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