Transcriptome analysis of tomato under salt stress induced by c-GMP

doi:10.3969/j.issn.1004-1524.2020.10.07

›› 2020, Vol. 32 ›› Issue (10): 1788-1797.DOI: 10.3969/j.issn.1004-1524.2020.10.07

• Horticultural Science • Previous Articles Next Articles

Transcriptome analysis of tomato under salt stress induced by c-GMP

ZHU Xiaolin^1,2,3, WEI Xiaohong^1,2,*, WANG Baoqiang^1,2,3, WANG Xian^1,2, ZHANG Mingjun^2,3

1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Gansu Key Lab of Crop Genetic & Germplasm Enhancement, Lanzhou 730070, China;
3. Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou 730070, China

Received:2020-01-18 Online:2020-10-25 Published:2020-11-16

Abstract

Abstract: Using tomato as material, Illumina high-throughput sequence technology was used to sequence the transcriptome of tomato seedlings under NaCl stress, c-GMP induction and the combination of the two. The results showed that: a total of 83.35 Gb of original data were obtained, and the percentage of Q30 bases was 90.91% and above. The original reading of each sample was compared with the designated reference genome, and the comparison efficiency ranged from 88.03% to 92.74%. At the same time, the 31 734 sequences obtained were annotated to different databases (GO, KEGG, KOG, NR, Pfam, Swiss-Prot and egg NOG), and they were divided into cell component, molecular function and biological process through the GO database. Among the 51 subfunctional groups under the big annotation category, they were annotated into 25 categories and 128 metabolic pathways through the COG database and the KEGG database, respectively. A total of 127 944 SNP sites were found under NaCl stress, c-GMP induction and the combination of the two treatments, of which only 22.6% of SNP sites were located in the non-coding region. The differential gene results showed that c-GMP could induce the expression of more genes under salt stress. The transcriptome sequence data was reliable and the results covered a wide range, providing a theoretical basis for gene mining and functional research of tomato under salt stress induced by c-GMP.

Key words: tomato, salt stress, c-GMP induction, transcriptome analysis

CLC Number:

S641.2

ZHU Xiaolin, WEI Xiaohong, WANG Baoqiang, WANG Xian, ZHANG Mingjun. Transcriptome analysis of tomato under salt stress induced by c-GMP[J]. , 2020, 32(10): 1788-1797.

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Transcriptome analysis of tomato under salt stress induced by c-GMP

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