Identification and biological characteristics of anthracnose pathogen on Tetrastigma hemsleyanum

doi:10.3969/j.issn.1004-1524.2020.11.11

›› 2020, Vol. 32 ›› Issue (11): 2009-2019.DOI: 10.3969/j.issn.1004-1524.2020.11.11

• Plant Protection • Previous Articles Next Articles

Identification and biological characteristics of anthracnose pathogen on Tetrastigma hemsleyanum

LI Xuqing^a, YAN Jianli^b,*, RUAN Songlin^c

a. Institute of Vegetable;
b. Branch of Science and Technology;
c. Institute of Crop (Ecology), Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2020-05-15 Online:2020-11-25 Published:2020-12-02

Abstract

Abstract: Leaf spot occurred severely in Chun'an city, Zhejiang Province, and caused serious economic losses in local Tetrastigma hemsleyanum production. To clarify the species and biological characteristics of the pathogen caused leaf spot could provide scientific data for the disease effective control. The pathogen was isolated by plate confrontation assay and identified as Colletotrichum gloeosporioides based on combination of morphological characters, molecular data (rDNA-ITS, β-tubulin, ACT, gpd, CaM)and pathogenicity tests. And the biological characteristics determined by crossing and haemocytometer methods showed that the fungus was able to grow at temperature between 20-35 ℃ with an optimum of 25 ℃ for both mycelia growth and sporulation; the continuous darkness was good for both mycelia growth and sporulation; In addition, the optimal pH for mycelia growth and sporulation was 5.0 and 8.0, respectively.There were various kinds of carbohydrates and nitrogen known to provide growth of the fungus. Maltose,sorbitol and yeast were the best carbon and nitrogen source for mycelia growth, respectively, while ethanol and glutamine were the worst, respectively. Sorbitol and yeast were the best carbon and nitrogen source for sporulation, respectively, while mannitol and casein tryptone were the worst, respectively.

Key words: Tetrastigma hemsleyanum, anthracnose, Colletotrichum gloeosporioides, identification of pathogen, biological characteristics

CLC Number:

S432.4⁺4

LI Xuqing, YAN Jianli, RUAN Songlin. Identification and biological characteristics of anthracnose pathogen on Tetrastigma hemsleyanum[J]. , 2020, 32(11): 2009-2019.

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Identification and biological characteristics of anthracnose pathogen on Tetrastigma hemsleyanum

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