Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.)

doi:10.3969/j.issn.1004-1524.2020.11.12

›› 2020, Vol. 32 ›› Issue (11): 2020-2026.DOI: 10.3969/j.issn.1004-1524.2020.11.12

• Plant Protection • Previous Articles Next Articles

Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.)

ZHANG Zheng^1,2, WANG Xiaorong³, QIAN Hong⁴, ZHANG Lan¹, YAN Peng¹, ZHANG Liping¹, ZHANG Xinfu², LI Xin^1,*, HAN Wenyan¹

1. Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China;
2. Horticulture Department, Qingdao Agricultural University, Qingdao 266109, China;
3. School of Economics and Management, Hebei University of Architecture, Zhangjiakou 075000, China;
4. Deqing Agricultural Technology Extension Center, Deqing 313200, China

Received:2020-03-03 Online:2020-11-25 Published:2020-12-02

Abstract

Abstract: Anthracnose caused by Colletotrichum camelliae is an important foliar disease that severely damages the yield and quality of tea. It occurs widely in most of the tea production areas in China. In this study, Longjing 43 and Zhongcha 108 were used as experimental materials to explore the effects of anthracnose on photosynthetic rate, Rubisco activity, photosynthetic genes expression and H₂O₂ content in tea leaves. It was revealed that there was no significant difference in photosynthetic rate between Longjing 43 and Zhongcha 108 under mock treatment. However, after inoculation with the pathogen, the photosynthetic rate decreased significantly along with the post-inoculation time. At 6 days post-inoculation, the photosynthetic rate of Longjing 43 and Zhongcha 108 decreased by 61.76% and 42.04%, maximum carboxylation rate of Rubisco (V_cmax) decreased by 33.78% and 20.22%, and maximum rates of RuBP regeneration (J_max) decreased by 37.67% and 20.83%, respectively. The expression of photosynthetic genes decreased significantly after inoculation. Notably, the expression of CsRbcL in the leaves of Longjing 43 and Zhongcha 108 decreased by 47.08% and 36.36%, and CsRbcS decreased by 34.99% and 23.90%, respectively. In addition, the content of H₂O₂ in the leaves of Longjing 43 and Zhongcha 108 increased by 174.91% and 96.69%, respectively. The study further confirmed that Longjing 43 was more susceptible to anthracnose than Zhongcha 108. It was also suggested that the decrease of leaf photosynthetic rate and Rubisco activity after pathogen inoculation was mainly attributed to the excessive accumulation of H₂O₂ in tea leaves.

Key words: anthracnose, Camellia sinensis, photosynthesis, hydrogen peroxide, gene expression

CLC Number:

S435.711

ZHANG Zheng, WANG Xiaorong, QIAN Hong, ZHANG Lan, YAN Peng, ZHANG Liping, ZHANG Xinfu, LI Xin, HAN Wenyan. Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.)[J]. , 2020, 32(11): 2020-2026.

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Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.)

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