Antimicrobial resistance in Salmonella and Campylobacter isolated from Zhejiang and Fujian Province in 2019

doi:10.3969/j.issn.1004-1524.2020.12.13

Acta Agriculturae Zhejiangensis ›› 2020, Vol. 32 ›› Issue (12): 2218-2225.DOI: 10.3969/j.issn.1004-1524.2020.12.13

• Quality and Safety of Agriculturel Products • Previous Articles Next Articles

Antimicrobial resistance in Salmonella and Campylobacter isolated from Zhejiang and Fujian Province in 2019

TANG Biao¹^,²(), CHEN Lingyun¹^,², LUO Yi¹^,²^,³, SHI Xingfen⁴, HU Ji², CHEN Yifei¹^,², QIAN Mingrong¹^,², YANG Hua¹^,²^,^*()

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products (in Prepared), Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Life Sciences, China Jiliang University, Hangzhou 310018, China
4. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310020, China

Received:2020-06-04 Online:2020-12-25 Published:2020-12-25
Contact: YANG Hua

Abstract

Abstract:

To understand the epidemicity and antimicrobial resistance of Salmonella and Campylobacter in Zhejiang and Fujian in 2019, 1 480 fecal and anal swab samples were randomly collected in 10 cities of Zhejiang Province and Fujian Province in 2019. Salmonella and Campylobacter strains were isolated by the method of national standards of China, and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was used for identification. The minimal inhibitory concentration (MIC) of Salmonella and Campylobacter strains against 16 and 9 antibiotics, respectively, was determined by microdilution broth method. A total of 100 Salmonella strains and 100 Campylobacter strains were isolated in the present study. Among them, 11 serotypes were identified in 94 Salmonella strains. The dominant serotypes were Tsevie (20.2%), Kentucky (15.9%), Weltevreden (15.9%), Typhimurium (14.9%) and Enteritidis (14.9%). The results of drug sensitivity test showed that the resistance rates of the isolated Salmonella strains to tetracycline was the highest (76%), which was followed by ampicillin (70%) and sulfamethoxazole (65%). While, the drug resistance rate of Salmonella strains to amoxicillin/clavulanate was low (6%), and all the isolated Salmonella strains were sensitive to meropenem. In addition, the isolated Campylobacter strains had the highest resistance rate to ciprofloxacin (99%), nalidixic acid (88%) and tetracycline (87%), while the resistance rate to gentamicin was the lowest (23%). All in all, the antimicrobial resistance level of pathogenic bacteria from animals was high in Zhejiang Province and Fujian Province, which would affect the quality and safety of animal products and threaten the public health safety.

Key words: Salmonella, Campylobacter, antimicrobial resistance

CLC Number:

S852.6

TANG Biao, CHEN Lingyun, LUO Yi, SHI Xingfen, HU Ji, CHEN Yifei, QIAN Mingrong, YANG Hua. Antimicrobial resistance in Salmonella and Campylobacter isolated from Zhejiang and Fujian Province in 2019[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2218-2225.

Figures/Tables 6

References 25

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序号 ID	耐药谱 Drug-resistant pattern	个数 Number	百分比 Percentage/%
1	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-CAZ-ENR-OFL	18	18.0
2	—	15	15.0
3	AMP-SPT-TET-FFC-SF-SXT	8	8.0
4	AMP-TET-SF	6	6.0
5	TET-FFC-SF-SXT	5	5.0
6	AMP-GEN-SPT-TET-FFC-SF-SXT	5	5.0
7	TET-FFC	5	5.0
8	AMP-GEN-TET	4	4.0
9	AMP-GEN-TET-FFC-SF-SXT	4	4.0
10	AMP-A/C-GEN-SPT-TET-FFC-SF-SXT	4	4.0
11	AMP-GEN-TET-CL	3	3.0
12	AMP-SPT-FFC-SXT-CEF-ENR	2	2.0
13	AMP-SF-CL	2	2.0
14	AMP-TET-SF-CL	2	2.0
15	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-ENR-OFL	2	2.0
16	AMP-TET-FFC-SF-SXT	2	2.0
17	TET	1	1.0
18	AMP-A/C-SPT-TET-SXT-CL	1	1.0
19	SPT	1	1.0
20	AMP-GEN-TET-FFC	1	1.0
21	AMP-GEN-CL	1	1.0
22	AMP-TET	1	1.0
23	TET-SF-CL	1	1.0
24	AMP-SPT-SF-SXT-CL	1	1.0
25	AMP-A/C-SPT-TET-SF-CEF-CAZ-CL	1	1.0
26	AMP-TET-FFC-SF-ENR	1	1.0
27	SF	1	1.0
28	AMP-SPT-FFC-SF-SXT	1	1.0
29	TET-SF-ENR	1	1.0

序号 ID	耐药谱 Drug-resistant pattern	个数 Number	百分比 Percentage/%
1	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-CAZ-ENR-OFL	18	18.0
2	—	15	15.0
3	AMP-SPT-TET-FFC-SF-SXT	8	8.0
4	AMP-TET-SF	6	6.0
5	TET-FFC-SF-SXT	5	5.0
6	AMP-GEN-SPT-TET-FFC-SF-SXT	5	5.0
7	TET-FFC	5	5.0
8	AMP-GEN-TET	4	4.0
9	AMP-GEN-TET-FFC-SF-SXT	4	4.0
10	AMP-A/C-GEN-SPT-TET-FFC-SF-SXT	4	4.0
11	AMP-GEN-TET-CL	3	3.0
12	AMP-SPT-FFC-SXT-CEF-ENR	2	2.0
13	AMP-SF-CL	2	2.0
14	AMP-TET-SF-CL	2	2.0
15	AMP-GEN-SPT-TET-FFC-SF-SXT-CEF-ENR-OFL	2	2.0
16	AMP-TET-FFC-SF-SXT	2	2.0
17	TET	1	1.0
18	AMP-A/C-SPT-TET-SXT-CL	1	1.0
19	SPT	1	1.0
20	AMP-GEN-TET-FFC	1	1.0
21	AMP-GEN-CL	1	1.0
22	AMP-TET	1	1.0
23	TET-SF-CL	1	1.0
24	AMP-SPT-SF-SXT-CL	1	1.0
25	AMP-A/C-SPT-TET-SF-CEF-CAZ-CL	1	1.0
26	AMP-TET-FFC-SF-ENR	1	1.0
27	SF	1	1.0
28	AMP-SPT-FFC-SF-SXT	1	1.0
29	TET-SF-ENR	1	1.0

序号 ID	耐药谱 Drug-resistant pattern			个数 Number	百分比 Percentage/%
1	CIP-NAL-TET			21	21.0
2	CIP-NAL-TET-CLI-ERY-AZM-TEL			11	11.0
3	CIP-TET-CLI-ERY-AZM-FFC			11	11.0
4	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL			8	8.0
5	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL-FFC			7	7.0
6	CIP-NAL-TET-CLI			5	5.0
7	CIP-NAL			5	5.0
8	CIP-NAL-TET-AZM			3	3.0
9	CIP-NAL-TET-FFC			3	3.0
10	CIP-NAL-CLI-AZM			3	3.0
11	CIP-NAL-TET-CLI-ERY-AZM-TEL-FFC			3	3.0
12	CIP-NAL-TET-CLI-AZM			2	2.0
13	CIP-NAL-TET-CLI-AZM-TEL			2	2.0
14	CIP-NAL-TET-CLI-AZM-TEL-FFC			2	2.0
15	CIP-NAL-GEN-CLI-ERY-AZM-TEL			2	2.0
16	CIP-NAL-GEN-TET-CLI-AZM-TEL-FFC			2	2.0
17		CIP-NAL-TET-TEL-FFC	1		1.0
18		CIP-NAL-TET-AZM-TEL	1		1.0
19		—	1		1.0
20		CIP-NAL-GEN-TET	1		1.0
21		CIP-NAL-GEN-TET-CLI-TEL	1		1.0
22		CIP-NAL-TET-CLI-TEL	1		1.0
23		CIP-NAL-GEN-TET-CLI	1		1.0
24		CIP-NAL-GEN-TET-CLI-ERY-AZM	1		1.0
25		CIP-NAL-TEL	1		1.0
26		CIP-NAL-CLI	1		1.0

序号 ID	耐药谱 Drug-resistant pattern			个数 Number	百分比 Percentage/%
1	CIP-NAL-TET			21	21.0
2	CIP-NAL-TET-CLI-ERY-AZM-TEL			11	11.0
3	CIP-TET-CLI-ERY-AZM-FFC			11	11.0
4	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL			8	8.0
5	CIP-NAL-GEN-TET-CLI-ERY-AZM-TEL-FFC			7	7.0
6	CIP-NAL-TET-CLI			5	5.0
7	CIP-NAL			5	5.0
8	CIP-NAL-TET-AZM			3	3.0
9	CIP-NAL-TET-FFC			3	3.0
10	CIP-NAL-CLI-AZM			3	3.0
11	CIP-NAL-TET-CLI-ERY-AZM-TEL-FFC			3	3.0
12	CIP-NAL-TET-CLI-AZM			2	2.0
13	CIP-NAL-TET-CLI-AZM-TEL			2	2.0
14	CIP-NAL-TET-CLI-AZM-TEL-FFC			2	2.0
15	CIP-NAL-GEN-CLI-ERY-AZM-TEL			2	2.0
16	CIP-NAL-GEN-TET-CLI-AZM-TEL-FFC			2	2.0
17		CIP-NAL-TET-TEL-FFC	1		1.0
18		CIP-NAL-TET-AZM-TEL	1		1.0
19		—	1		1.0
20		CIP-NAL-GEN-TET	1		1.0
21		CIP-NAL-GEN-TET-CLI-TEL	1		1.0
22		CIP-NAL-TET-CLI-TEL	1		1.0
23		CIP-NAL-GEN-TET-CLI	1		1.0
24		CIP-NAL-GEN-TET-CLI-ERY-AZM	1		1.0
25		CIP-NAL-TEL	1		1.0
26		CIP-NAL-CLI	1		1.0

Antimicrobial resistance in Salmonella and Campylobacter isolated from Zhejiang and Fujian Province in 2019

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