Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 454-463.DOI: 10.3969/j.issn.1004-1524.2021.03.10
• Horticultrual Science • Previous Articles Next Articles
Analysis on alkaloids metabolites of two edible roses
QIAN Xiaohui, CHEN Longqing, LI Shuangqin, SHI Rui*(
)
- Functional Flower Resources of Yunnan Province and Engineering Research Center of Industrial Technology, College of Horticulture and Landscape Architecture, Southwest Forestry University, Kunming 650224, China
-
Received:2020-08-10Online:2021-03-25Published:2021-03-25 -
Contact:SHI Rui
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QIAN Xiaohui, CHEN Longqing, LI Shuangqin, SHI Rui. Analysis on alkaloids metabolites of two edible roses[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 454-463.
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Fig.1 2D PCA and 3D PCA of each sample ZY represented Rosa gallica L. cv. Dianhong, DH represented Rosa chinensis Jacq.cv. Crimson Glory, Mix01, Mix02 and Mix03 represented equivalent mixtures sample of ZY and DH.
Fig.1 2D PCA and 3D PCA of each sample ZY represented Rosa gallica L. cv. Dianhong, DH represented Rosa chinensis Jacq.cv. Crimson Glory, Mix01, Mix02 and Mix03 represented equivalent mixtures sample of ZY and DH.
Table 1 All PCA results in DH and ZY
| 名称Name | 成分1 PC1 | 成分2 PC2 | 成分3 PC3 | 成分4 PC4 | 成分5 PC5 |
|---|---|---|---|---|---|
| ZY | -9.16 | 4.36 | 1.39 | 0.15 | -4.48×10-16 |
| DH | 9.49 | 2.37 | 3.55 | -0.17 | -4.32×10-16 |
| Mix01 | -3.06 | -5.83 | 3.00 | 0.63 | 2.83×10-15 |
| Mix02 | 2.20 | 0.01 | -4.53 | 3.15 | 1.58×10-15 |
| Mix03 | 0.54 | -0.91 | -3.41 | -3.75 | 2.12×10-15 |
Table 1 All PCA results in DH and ZY
| 名称Name | 成分1 PC1 | 成分2 PC2 | 成分3 PC3 | 成分4 PC4 | 成分5 PC5 |
|---|---|---|---|---|---|
| ZY | -9.16 | 4.36 | 1.39 | 0.15 | -4.48×10-16 |
| DH | 9.49 | 2.37 | 3.55 | -0.17 | -4.32×10-16 |
| Mix01 | -3.06 | -5.83 | 3.00 | 0.63 | 2.83×10-15 |
| Mix02 | 2.20 | 0.01 | -4.53 | 3.15 | 1.58×10-15 |
| Mix03 | 0.54 | -0.91 | -3.41 | -3.75 | 2.12×10-15 |
Fig.2 Number of different types of metabolites identified in all samples
Fig.2 Number of different types of metabolites identified in all samples
Fig.3 Heat map of DH, ZH and Mix The columns represented different samples and the rows represented different metabolites; the depth of color represented the value of the correlation coefficient.
Fig.3 Heat map of DH, ZH and Mix The columns represented different samples and the rows represented different metabolites; the depth of color represented the value of the correlation coefficient.
Fig.4 The top 20 metabolites with fold change between ZY and DH Green represented down-regulated differential metabolites, red represented up-regulated differential metabolites, and the value represented the multiple of difference.
Fig.4 The top 20 metabolites with fold change between ZY and DH Green represented down-regulated differential metabolites, red represented up-regulated differential metabolites, and the value represented the multiple of difference.
Table 2 The significantly different metabolites in DH and ZY
| 序号 No. | 化合物 Compounds | 滇红 ZY/s-1 | 墨红 DH/s-1 | 差异倍数值 Fold change | 代谢物分组 Grouping of metabolites | 调控类型 Regulation type |
|---|---|---|---|---|---|---|
| 1 | L-天冬酰胺L-Asparagine | — | 1.34×107 | 6.73×10-7 | DH | 下调Down |
| 2 | 烟酸甲酯 | 3.93×105 | 7.23×106 | 5.43×10-2 | ZY、DH | 下调Down |
| Nicotinic Acid Methyl Ester(Methyl Nicotinate) | ||||||
| 3 | L-(-)-苏氨酸 L-(-)-Threonine | 5.88×105 | 2.23×106 | 2.63×10-1 | ZY、DH | 下调Down |
| 4 | L-(+)-精氨酸 L-(+)-Arginine | 1.37×106 | 6.55×106 | 2.09×10-1 | ZY、DH | 下调Down |
| 5 | N-乙酰-L-酪氨酸N-Acetyl-L-tyrosine | 3.46×104 | 1.35×104 | 2.56 | ZY、DH | 上调Up |
| 6 | O-乙酰丝氨酸O-Acetylserine | 1.41×105 | 3.06×105 | 4.61×10-1 | ZY、DH | 下调Down |
| 7 | DL-2-氨基己二酸DL-2-Aminoadipic acid | 7.49×105 | 1.86×106 | 4.02×10-1 | ZY、DH | 下调Down |
| 8 | D-(-)-缬氨酸 D-(-)-Valine | 1.45×106 | 2.99×106 | 4.84×10-1 | ZY、DH | 下调Down |
| 9 | L-瓜氨酸 L-Citrulline | 2.09×105 | 5.04×105 | 4.14×10-1 | ZY、DH | 下调Down |
| 10 | L-谷氨酸L-Glutamic acid | 1.61×106 | 1.04×107 | 1.54×10-1 | ZY、DH | 下调Down |
| 11 | L-(+)-赖氨酸L-(+)-Lysine | 4.09×105 | 1.65×106 | 2.48×10-1 | ZY、DH | 下调Down |
| 12 | DL-高半胱氨酸DL-Homocysteine | 8.19×103 | 1.85×104 | 4.42×10-1 | ZY、DH | 下调Down |
| 13 | N6-乙酰-L-赖氨酸N6-Acetyl-L-lysine | 5.29×105 | 2.16×106 | 2.45×10-1 | ZY、DH | 下调Down |
| 14 | 甘氨酰-L-脯氨酸Glycyl-L-proline | 2.53×104 | 7.20×104 | 3.52×10-1 | ZY、DH | 下调Down |
| 15 | L-谷氨酰胺L-Glutamine | 5.14×105 | 2.42×106 | 2.12×10-1 | ZY、DH | 下调Down |
| 16 | 谷胱甘肽Glutathione | 1.09×106 | 4.12×104 | 2.64×101 | ZY、DH | 上调Up |
| 17 | S-(5'-腺苷)-L-高半胱氨酸 | — | 6.92×104 | 1.30×10-4 | DH | 下调Down |
| S-(5'-Adenosy)-L-homocysteine | ||||||
| 18 | 3-羟丙基棕榈酸酯葡萄糖胺 | 3.61×106 | 1.05×106 | 3.45 | ZY、DH | 上调Up |
| 3-Hydroxypropyl palmitate glc-glucosamine |
Table 2 The significantly different metabolites in DH and ZY
| 序号 No. | 化合物 Compounds | 滇红 ZY/s-1 | 墨红 DH/s-1 | 差异倍数值 Fold change | 代谢物分组 Grouping of metabolites | 调控类型 Regulation type |
|---|---|---|---|---|---|---|
| 1 | L-天冬酰胺L-Asparagine | — | 1.34×107 | 6.73×10-7 | DH | 下调Down |
| 2 | 烟酸甲酯 | 3.93×105 | 7.23×106 | 5.43×10-2 | ZY、DH | 下调Down |
| Nicotinic Acid Methyl Ester(Methyl Nicotinate) | ||||||
| 3 | L-(-)-苏氨酸 L-(-)-Threonine | 5.88×105 | 2.23×106 | 2.63×10-1 | ZY、DH | 下调Down |
| 4 | L-(+)-精氨酸 L-(+)-Arginine | 1.37×106 | 6.55×106 | 2.09×10-1 | ZY、DH | 下调Down |
| 5 | N-乙酰-L-酪氨酸N-Acetyl-L-tyrosine | 3.46×104 | 1.35×104 | 2.56 | ZY、DH | 上调Up |
| 6 | O-乙酰丝氨酸O-Acetylserine | 1.41×105 | 3.06×105 | 4.61×10-1 | ZY、DH | 下调Down |
| 7 | DL-2-氨基己二酸DL-2-Aminoadipic acid | 7.49×105 | 1.86×106 | 4.02×10-1 | ZY、DH | 下调Down |
| 8 | D-(-)-缬氨酸 D-(-)-Valine | 1.45×106 | 2.99×106 | 4.84×10-1 | ZY、DH | 下调Down |
| 9 | L-瓜氨酸 L-Citrulline | 2.09×105 | 5.04×105 | 4.14×10-1 | ZY、DH | 下调Down |
| 10 | L-谷氨酸L-Glutamic acid | 1.61×106 | 1.04×107 | 1.54×10-1 | ZY、DH | 下调Down |
| 11 | L-(+)-赖氨酸L-(+)-Lysine | 4.09×105 | 1.65×106 | 2.48×10-1 | ZY、DH | 下调Down |
| 12 | DL-高半胱氨酸DL-Homocysteine | 8.19×103 | 1.85×104 | 4.42×10-1 | ZY、DH | 下调Down |
| 13 | N6-乙酰-L-赖氨酸N6-Acetyl-L-lysine | 5.29×105 | 2.16×106 | 2.45×10-1 | ZY、DH | 下调Down |
| 14 | 甘氨酰-L-脯氨酸Glycyl-L-proline | 2.53×104 | 7.20×104 | 3.52×10-1 | ZY、DH | 下调Down |
| 15 | L-谷氨酰胺L-Glutamine | 5.14×105 | 2.42×106 | 2.12×10-1 | ZY、DH | 下调Down |
| 16 | 谷胱甘肽Glutathione | 1.09×106 | 4.12×104 | 2.64×101 | ZY、DH | 上调Up |
| 17 | S-(5'-腺苷)-L-高半胱氨酸 | — | 6.92×104 | 1.30×10-4 | DH | 下调Down |
| S-(5'-Adenosy)-L-homocysteine | ||||||
| 18 | 3-羟丙基棕榈酸酯葡萄糖胺 | 3.61×106 | 1.05×106 | 3.45 | ZY、DH | 上调Up |
| 3-Hydroxypropyl palmitate glc-glucosamine |
Fig.5 Statistic of KEGG enrichment The size of the dot indicated the amount of enriched metabolites, the color indicated the significant value, and the enrichment factor indicated that the ratio of the number of metabolites differentially expressed in the corresponding pathway to the total number of metabolites detected and annotated by the pathway.
Fig.5 Statistic of KEGG enrichment The size of the dot indicated the amount of enriched metabolites, the color indicated the significant value, and the enrichment factor indicated that the ratio of the number of metabolites differentially expressed in the corresponding pathway to the total number of metabolites detected and annotated by the pathway.
Fig.6 Differential metabolites in Biosynthesis of amino acids pathways in DH and ZY Green lines represented significant down-regulated differential metabolites, blue dotted line represented the detected metabolites with no significant difference.
Fig.6 Differential metabolites in Biosynthesis of amino acids pathways in DH and ZY Green lines represented significant down-regulated differential metabolites, blue dotted line represented the detected metabolites with no significant difference.
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