Study on fermentation conditions of herbicidal active strain HL-1

doi:10.3969/j.issn.1004-1524.2021.06.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (6): 1042-1048.DOI: 10.3969/j.issn.1004-1524.2021.06.09

• Plant Protection • Previous Articles Next Articles

Study on fermentation conditions of herbicidal active strain HL-1

GAO Hanfeng(), LIU Yuqin, CHENG Liang, GUO Qingyun^*()

Institute of Plant Protection, Academy of Agriculture and Forestry Science, Qinghai University, Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Scientific Observing and Experimental Station of Crop Pest of Ministry of Agriculture and Rural Affairs in Xining, Xining 810016, China

Received:2020-06-29 Online:2021-06-25 Published:2021-06-25
Contact: GUO Qingyun

Abstract

Abstract:

Taking endophytic fungus HL-1 strain with herbicidal activity isolated from Qinghai diseased Cirsium setosum as the material, a single factor test was used to screen the carbon and nitrogen sources, solid fermentation substrate, inoculation amount and solid-liquid fermentation conditions that were most suitable for the growth of the strain. The results showed that the most suitable carbon source of HL-1 in the tested materials was glucose and the nitrogen source was yeast extract; when the strain was cultured in PDA medium, its maximum spore production was 5.79×10⁸ CFU·mL^-1 ; and in the optimal carbon source and optimal nitrogen source mixed medium, its maximum spore production was 4.81×10⁹ CFU·mL^-1. Rapeseed cake was the medium material with the largest spore production of HL-1 in the test materials, which had the largest spore production at a volume fraction of 40% inoculation volume. Through orthogonal optimization experiments, the spore production of the strain was found to be the largest under the conditions of culture temperature 27.5 ℃, culture medium water content 37% and fermentation time 8 d.

Key words: endophytic fungus HL-1, solid fermentation substrate, solid-liquid fermentation conditions

CLC Number:

S476.1

GAO Hanfeng, LIU Yuqin, CHENG Liang, GUO Qingyun. Study on fermentation conditions of herbicidal active strain HL-1[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1042-1048.

Figures/Tables 7

References 20

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水平 Level	培养条件Culture condition
水平 Level	培养基含水量 Medium water content/%	培养温度 Culture temperature/℃	培养时间 Culture time/d
上星号臂(+1.6818) Upper asterisk arm (+1.6818)	65	35	14
上水平(+1) Upper level(+1)	58	32	12
零水平(0) Zero level(0)	47.5	27.5	8
下水平(-1) Lower level(-1)	37	23	5
下星号臂(-1.6818) Lower asterisk arm (-1.6818)	30	20	3

水平 Level	培养条件Culture condition
水平 Level	培养基含水量 Medium water content/%	培养温度 Culture temperature/℃	培养时间 Culture time/d
上星号臂(+1.6818) Upper asterisk arm (+1.6818)	65	35	14
上水平(+1) Upper level(+1)	58	32	12
零水平(0) Zero level(0)	47.5	27.5	8
下水平(-1) Lower level(-1)	37	23	5
下星号臂(-1.6818) Lower asterisk arm (-1.6818)	30	20	3

碳氮源 Carbon and nitrogen sources	HL-1菌株的菌落形态 Colony morphology of HL-1 strain
C1	白色的菌落,没有同心轮纹,发达的气生菌丝 White colony without concentric ring pattern, developed aerial mycelium
C2	乳白色的菌落,具有同心轮纹,较少的气生菌丝 Milky white colony with concentric ring pattern, less aerial mycelium
C3	纯白色的菌落,具有同心状轮纹,发达的气生菌丝 Pure white colony with concentric ring pattern, developed aerial mycelium
C4	纯白色的菌落,具有同心轮纹,发达的气生菌丝且铺满整皿 Pure white colony with concentric ring pattern, developed aerial mycelium and full petrie dish
N1	菌落表面有乳白色的绒毛状菌丝,菌落同心轮纹,边缘呈带丝状,基内菌丝呈褐色 Milky white hairy mycelium on the surface of the colony, concentric ring colony with filamentous edge, brown subtrate mycelium
N2	白色的菌丝,基内菌丝部分呈灰褐色,发达的气生菌丝和基内菌丝 White mycelium, gray-brown and developed substrate mycelium, developed aerial mycelium
N3	较小的菌落形态,灰褐色的气生菌丝且表面有隆起,发达的基内菌丝 Small colony morphology, gray-brown aerial mycelium with raised surface, developed substrate mycelium
N4	较大的菌落形态,乳白色的气生菌丝,具有同心轮纹,菌落边缘呈带丝状,黄褐色的基内菌丝 Large colony morphology, milky white aerial mycelium,concentric ring pattern, filamentous edge, yellow-brown substrate mycelium

碳氮源 Carbon and nitrogen sources	HL-1菌株的菌落形态 Colony morphology of HL-1 strain
C1	白色的菌落,没有同心轮纹,发达的气生菌丝 White colony without concentric ring pattern, developed aerial mycelium
C2	乳白色的菌落,具有同心轮纹,较少的气生菌丝 Milky white colony with concentric ring pattern, less aerial mycelium
C3	纯白色的菌落,具有同心状轮纹,发达的气生菌丝 Pure white colony with concentric ring pattern, developed aerial mycelium
C4	纯白色的菌落,具有同心轮纹,发达的气生菌丝且铺满整皿 Pure white colony with concentric ring pattern, developed aerial mycelium and full petrie dish
N1	菌落表面有乳白色的绒毛状菌丝,菌落同心轮纹,边缘呈带丝状,基内菌丝呈褐色 Milky white hairy mycelium on the surface of the colony, concentric ring colony with filamentous edge, brown subtrate mycelium
N2	白色的菌丝,基内菌丝部分呈灰褐色,发达的气生菌丝和基内菌丝 White mycelium, gray-brown and developed substrate mycelium, developed aerial mycelium
N3	较小的菌落形态,灰褐色的气生菌丝且表面有隆起,发达的基内菌丝 Small colony morphology, gray-brown aerial mycelium with raised surface, developed substrate mycelium
N4	较大的菌落形态,乳白色的气生菌丝,具有同心轮纹,菌落边缘呈带丝状,黄褐色的基内菌丝 Large colony morphology, milky white aerial mycelium,concentric ring pattern, filamentous edge, yellow-brown substrate mycelium

碳氮源处理 Carbon and nitrogen source treatments	孢子平均值 Average value of spores/(CFU·mL^-1)	D值 D value
C1	2.30×10⁶	0.110 dD
C2	1.95×10⁶	0.009 dDE
C3	1.05×10⁷	0.250 cC
C4	1.50×10⁸	0.517 bB
N1	4.60×10⁶	0.069 dDE
N2	3.90×10⁶	0.033 deDE
N3	2.20×10⁹	0.650 aA
N4	2.25×10⁵	0.008 eE
PDA	5.79×10⁸	0.528 bB
C4+N3	4.81×10⁹	0.691 aA

Study on fermentation conditions of herbicidal active strain HL-1

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试验号 No.	含水量 Water content	温度 Temperature	培养时间 Culture time	lgCFU均值 lgCFU average/%
1	1	1	1	12.3
2	1	1	-1	31.3
3	1	-1	1	24.0
4	1	-1	-1	17.4
5	-1	1	1	35.7
6	-1	1	-1	32.5
7	-1	-1	1	40.0
8	-1	-1	-1	45.1
9	-1.6818	0	0	23.0
10	1.6818	0	0	31.1
11	0	-1.6818	0	14.0
12	0	1.6818	0	17.2
13	0	0	-1.6818	24.3
14	0	0	1.6818	31.2
15	0	0	0	21.5
16	0	0	0	24.1
17	0	0	0	28.0
18	0	0	0	29.2
19	0	0	0	25.0
20	0	0	0	23.4
21	0	0	0	25.0
22	0	0	0	28.1
23	0	0	0	22.0

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