Performance of trehalose treatment on yield formation and quality of post-flowering heat-stressed weak gluten wheat Shengxuan No.6

doi:10.3969/j.issn.1004-1524.2023.01.01

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (1): 1-9.DOI: 10.3969/j.issn.1004-1524.2023.01.01

• Crop Science • Previous Articles Next Articles

Performance of trehalose treatment on yield formation and quality of post-flowering heat-stressed weak gluten wheat Shengxuan No.6

WANG Ben(), LI Yuxing, LI Zhe, JIANG Fengyi, HUANG Zhenglai(), FAN Yonghui, ZHANG Wenjing, MA Shangyu

College of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture and Rural Affairs, Hefei 230036, China

Received:2021-09-30 Online:2023-01-25 Published:2023-02-21

Abstract

Abstract:

To determine the effect of treatment with trehalose on yield formation and quality of post-flowering heat-stressed weak gluten wheat, the experiment was conducted in 2020-2021 at the National Agricultural Science and Technology Park in Huainan, Anhui Province, with high-quality weakly glutenous wheat Shengxuan No. 6 as the test material. The experiment was carried out at heading stage (T1), flowering stage (T2) and 10 days after flowering stage (T3). Treatment concentrations of trehalose were 5 mmol·L^-1 (C1), 10 mmol·L^-1 (C2), and 15 mmol·L^-1 (C3), with equal amounts of water sprayed as control, high temperature treatment was applied 15-19 d after flowering. The analysis showed that trehalose treatment increased grain yield of wheat under post-anthesis high temperature stress and improved the dry matter allocation and proportion of grains, and the dry matter allocation and proportion of nutrient organs displayed a decreasing trend after trehalose treatment, while the transport amount and rate of dry matter stored in nutrient organs before flowering to the grains and the accumulation of dry matter after flowering showed an increasing trend. With the increasing concentration of trehalose treatment the starch content of the grains also showed an increasing trend, and no significant difference was found between the two treatment levels of C2 and C3 in various treatment periods, however, the level of C3 was slightly higher than C2. Trehalose treatment reduced the content of grain albumin, globulin and gliadin protein, but the content of glutenin was higher than CK, and the glutein/gliadin value of the grains under trehalose treatment was significantly higher than CK. The trehalose-treated kernels were significantly different from CK in terms of protein content, wet gluten content, formation time, stabilization time and sedimentation value. The greatest improvement in grain yield and quality was achieved by spraying C3 concentration of trehalose at T1 period compared with CK under the study conditions of this experiment.

Key words: high temperature stress, weakly glutenous wheat, trehalose, yield formation, quality

CLC Number:

S512.1

WANG Ben, LI Yuxing, LI Zhe, JIANG Fengyi, HUANG Zhenglai, FAN Yonghui, ZHANG Wenjing, MA Shangyu. Performance of trehalose treatment on yield formation and quality of post-flowering heat-stressed weak gluten wheat Shengxuan No.6[J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 1-9.

Figures/Tables 10

References 35

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花后天数 Day after anthesis/d	处理时平均温 Average temperature at treatment/℃	处理时棚外平均温 Control mean temperature at treatment/℃	温度差 Temperature difference/℃
15	28.1	18.8	9.3
16	29.5	20.4	9.1
17	32.5	24.4	8.1
18	31.0	20.7	10.3
19	29.3	20.1	9.2

花后天数 Day after anthesis/d	处理时平均温 Average temperature at treatment/℃	处理时棚外平均温 Control mean temperature at treatment/℃	温度差 Temperature difference/℃
15	28.1	18.8	9.3
16	29.5	20.4	9.1
17	32.5	24.4	8.1
18	31.0	20.7	10.3
19	29.3	20.1	9.2

喷施时期 Application period	处理 Treatment	籽粒 Grain		茎鞘+叶片 Steam and sheath and leaf		颖壳+穗轴 Glume and spike axis
喷施时期 Application period	处理 Treatment	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%
T1	CK	1.20 f	41.86 d	1.28 a	43.87 a	0.38 c	13.27 d
	C1	1.26 e	43.40 c	1.28 a	43.49 c	0.35 e	12.15 i
	C2	1.34 b	44.96 b	1.26 b	41.69 g	0.37 d	12.42 h
	C3	1.43 a	47.49 a	1.23 e	41.11 i	0.34 f	11.29 l
T2	CK	1.19 f	42.78 cd	1.24 d	43.72 b	0.35 e	12.52 f
	C1	1.20 f	41.84 d	1.25 c	43.10 d	0.40 b	14.10 b
	C2	1.27 e	45.23 b	1.19 g	41.39 h	0.35 e	12.47 g
	C3	1.33 bc	47.27 a	1.02 h	35.78 l	0.45 a	16.16 a
T3	CK	1.20 f	42.49 cd	1.22 e	42.55 e	0.39 b	13.99 c
	C1	1.27 de	45.55 b	1.19 f	41.82 f	0.33 g	11.72 k
	C2	1.33 bc	46.56 a	1.18 g	40.54 k	0.34 f	12.00 j
	C3	1.30 cd	45.37 b	1.20 f	40.97 j	0.37 d	12.77 e
T		31.59^**	5.59^*	2 236.46^**	2 398.50^**	527.80^**	30 965.22^**
C		137.58^**	111.68^**	1 649.05^**	9 724.94^**	327.29^**	9 749.92^**
T×C		12.57^**	17.09^**	779.78^**	2 674.30^**	722.73^**	23 581.74^**

喷施时期 Application period	处理 Treatment	籽粒 Grain		茎鞘+叶片 Steam and sheath and leaf		颖壳+穗轴 Glume and spike axis
喷施时期 Application period	处理 Treatment	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%
T1	CK	1.20 f	41.86 d	1.28 a	43.87 a	0.38 c	13.27 d
	C1	1.26 e	43.40 c	1.28 a	43.49 c	0.35 e	12.15 i
	C2	1.34 b	44.96 b	1.26 b	41.69 g	0.37 d	12.42 h
	C3	1.43 a	47.49 a	1.23 e	41.11 i	0.34 f	11.29 l
T2	CK	1.19 f	42.78 cd	1.24 d	43.72 b	0.35 e	12.52 f
	C1	1.20 f	41.84 d	1.25 c	43.10 d	0.40 b	14.10 b
	C2	1.27 e	45.23 b	1.19 g	41.39 h	0.35 e	12.47 g
	C3	1.33 bc	47.27 a	1.02 h	35.78 l	0.45 a	16.16 a
T3	CK	1.20 f	42.49 cd	1.22 e	42.55 e	0.39 b	13.99 c
	C1	1.27 de	45.55 b	1.19 f	41.82 f	0.33 g	11.72 k
	C2	1.33 bc	46.56 a	1.18 g	40.54 k	0.34 f	12.00 j
	C3	1.30 cd	45.37 b	1.20 f	40.97 j	0.37 d	12.77 e
T		31.59^**	5.59^*	2 236.46^**	2 398.50^**	527.80^**	30 965.22^**
C		137.58^**	111.68^**	1 649.05^**	9 724.94^**	327.29^**	9 749.92^**
T×C		12.57^**	17.09^**	779.78^**	2 674.30^**	722.73^**	23 581.74^**

喷施时期 Application period	处理 Treatment	花前干物质转运 Pre-anthesis dry matter translocation			花后干物质积累 Post-anthesis dry matter accumulation
喷施时期 Application period	处理 Treatment	转运量 Translocation into grain/(kg·hm^-2)	转运率 Translocation ratio/%	对籽粒干物质贡献率 Contribution to grain/%	积累量 Assimilation amount/(kg·hm^-2)	对籽粒干物质贡献率 Contribution to grain/%
T1	CK	2 724.95 j	20.49 j	33.39 i	5 438.06 c	66.61 b
	C1	2 878.55 i	21.57 i	33.53 hi	5 708.35 b	66.47 bc
	C2	2 892.10 i	21.66 i	31.71 j	6 229.67 a	68.29 a
	C3	3 352.88 f	24.90 f	34.33 h	6 422.11 a	65.67 c
喷施时期 Application period	处理 Treatment	花前干物质转运 Pre-anthesis dry matter translocation			花后干物质积累 Post-anthesis dry matter accumulation
喷施时期 Application period	处理 Treatment	转运量 Translocation into grain/(kg·hm^-2)	转运率 Translocation ratio/%	对籽粒干物质贡献率 Contribution to grain/%	积累量 Assimilation amount/(kg·hm^-2)	对籽粒干物质贡献率 Contribution to grain/%
T2	CK	3 181.22 g	23.70 g	39.13 e	4 950.75 d	60.87 f
	C1	2 738.51 j	20.58 j	33.57 hi	5 420.39 c	66.43 bc
	C2	3 560.68 d	26.36 d	41.09 c	5 106.94 d	58.91 h
	C3	3 967.25 a	29.22 a	43.79 a	5 093.91 d	56.21 j
T3	CK	2 996.00 h	22.39 h	36.66 g	5 178.66 d	63.34 d
	C1	3 678.13 b	27.19 b	42.29 b	5 020.58 d	57.71 i
	C2	3 641.99 c	26.93 c	40.14 d	5 433.20 c	59.86 g
	C3	3 379.98 e	25.09 e	38.05 f	5 503.81 bc	61.95 e
T		5 075.23^**	3 896.35^**	670.48^**	146.17^**	670.38^**
C		4 368.38^**	3 353.54^**	49.66^**	27.89^**	49.65^**
T×C		2 342.76^**	1 798.68^**	150.84^**	15.27^**	150.82^**

Performance of trehalose treatment on yield formation and quality of post-flowering heat-stressed weak gluten wheat Shengxuan No.6

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喷施时期 Application period	处理 Treatment	清蛋白 Albumin/%	球蛋白 Globulin/%	醇溶蛋白 Gliadin/%	谷蛋白 Glutein/%	谷/醇 Glutein/gliadin
T1	CK	3.19±0.01 a	2.37±0.01 a	2.34±0.06 a	2.80±0.03 i	1.20±0.03 g
	C1	2.97±0.04 b	2.07±0.01 e	1.94±0.05 b	3.00±0.03 f	1.55±0.03 f
	C2	2.83±0.04 cd	1.92±0.01 h	1.72±0.06 d	3.10±0.03 c	1.81±0.06 d
	C3	2.77±0.04 d	1.77±0.01 j	1.52±0.06 f	3.20±0.03 a	2.12±0.08 a
T2	CK	3.19±0.01 a	2.34±0.02 b	2.34±0.06 a	2.80±0.03 i	1.20±0.03 g
	C1	3.01±0.04 b	2.17±0.01 c	1.96±0.06 b	2.95±0.03 h	1.50±0.04 f
	C2	2.88±0.03 c	2.06±0.01 e	1.79±0.05 c	3.02±0.03 e	1.69±0.04 e
	C3	2.80±0.04 cd	1.97±0.01 g	1.62±0.04 e	3.10±0.03 c	1.92±0.03 c
T3	CK	3.19±0.01 a	2.37±0.01 a	2.34±0.06 a	2.80±0.03 i	1.20±0.03 g
	C1	2.98±0.04 b	2.12±0.01 d	1.95±0.06 b	2.97±0.03 g	1.53±0.04 f
	C2	2.85±0.04 cd	2.00±0.01 f	1.75±0.06 d	3.06±0.03 d	1.75±0.05 d
	C3	2.79±0.04 d	1.87±0.01 i	1.59±0.04 e	3.15±0.03 b	1.98±0.03 b
T		1.13	154.34^**	16.39^**	1 290.30^**	21.24^**
C		151.54^**	1 907.43^**	2 329.44^**	27 761.85^**	950.76^**
T×C		0.15	33.38^**	4.10^**	186.63^**	5.50^**

喷施时期 Application period	处理 Treatment	蛋白质 Protein content/%	容重 Bulk density/g	湿面筋 Wet gluten/%	形成时间 Formation time/min	稳定时间 Stabilization time/min	沉降值 Sedimentation value/mL
T1	CK	12.76±0.09 a	792.00±1.25 bc	26.14±0.17 a	7.60±0.05 a	2.87±0.03 a	20.53±0.50 a
	C1	12.16±0.09 c	790.67±0.27 bcde	23.58±0.17 c	6.90±0.14 b	2.27±0.03 c	16.47±0.05 b
	C2	11.86±0.09 e	788.00±1.63 e	21.69±0.17 e	6.07±0.03 cd	1.97±0.03 e	14.17±0.17 c
	C3	11.58±0.05 g	792.00±0.47 bc	21.37±0.18 g	5.37±0.19 e	1.70±0.05 g	12.50±0.08 d
T2	CK	12.76±0.09 a	791.67±1.19 bcd	26.14±0.17 a	7.60±0.05 a	2.87±0.03 a	20.53±0.50 a
	C1	12.22±0.09 b	792.00±1.25 bc	23.68±0.17 b	7.05±0.14 b	2.39±0.03 b	16.62±0.05 b
	C2	11.93±0.09 d	788.33±0.54 cde	22.44±0.17 d	6.28±0.03 c	2.15±0.03 d	14.33±0.17 c
	C3	11.79±0.09 f	791.00±0.00 bcde	21.68±0.17 e	5.59±0.19 e	1.91±0.03 ef	12.64±0.08 d
T3	CK	12.76±0.09 a	799.00±0.00 a	26.11±0.19 a	7.57±0.05 a	2.87±0.03 a	20.53±0.50 a
	C1	12.20±0.09 bc	791.67±1.19 bcd	23.75±0.19 b	7.02±0.14 b	2.36±0.03 b	16.59±0.05 b
	C2	11.93±0.09 d	792.33±0.98 b	22.45±0.19 d	6.30±0.03 c	2.10±0.03 d	14.33±0.17 c
	C3	11.75±0.09 f	794.33±0.72 b	21.58±0.19 f	5.73±0.05 de	1.85±0.03 f	12.62±0.08 d
T		31.90^**	14.49^**	109.87^**	2.32	26.40^**	0.18
C		2536.05^**	9.42^**	12 625.54^**	160.95^**	933.22^**	421.18^**
T×C		8.46^**	2.44^*	39.40^**	0.52	3.46^*	0.02

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