Flower bud formation and physiological biochemistry characteristics of Hongmeiren citrus hybrid in northern Zhejiang, China

doi:10.3969/j.issn.1004-1524.20221126

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (7): 1571-1581.DOI: 10.3969/j.issn.1004-1524.20221126

• Horticultural Science • Previous Articles Next Articles

Flower bud formation and physiological biochemistry characteristics of Hongmeiren citrus hybrid in northern Zhejiang, China

WANG Zhihao¹(), XI Xinyan¹, WANG Li², YANG Shuna¹, GAO Zhiyuan¹, YIN Yiming², ZOU Hui³, JIA Huijuan¹^,^*()

1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
2. Agricultural Technology Development Center of Huzhou, Huzhou 313000, Zhejiang, China
3. Nanxun Shuanglin Deyuan Family Farm, Huzhou 313012, Zhejiang, China

Received:2022-08-02 Online:2023-07-25 Published:2023-08-17
Contact: JIA Huijuan

Abstract

Abstract:

In order to reveal the flower bud differentiation rules and physiological biochemistry characteristics of different female branches of Hongmeiren citrus hybrid, and provide theoretical basis for the regulation of flowering and cultivation management. 6-year-old Hongmeiren citrus hybrid trees were used as materials. The frozen section technology was used to observe the morphological characteristics. In addition, endogenous hormones [abscisic acid (ABA), trans-zeatin-riboside (ZR), gibberellin (GA) and indole-3-acetic acid (IAA)] were determined by LC-MS and the leaves were dried for total carbon and nitrogen content determination. The morphological differentiation of flower buds was divided into 6 stages, including undifferentiated stage, initial differentiation stage, sepal differentiation stage, petal differentiation stage, stamen differentiation stage and pistil differentiation stage. The general trend was that the flower bud differentiation process of the front end shoots was faster than that of the middle and rear end shoots, and the proportion of flower bud in the pistil differentiation stage was higher. In three shoots, the carbon/nitrogen ratio(C/N), ABA/GA, ZR/GA, IAA/GA of autumn shoot in spring shoot and summer shoot and autumn shoot (SSA-AU) were higher than that of summer shoot in spring shoot and summer shoot and autumn shoot (SSA-SU) and spring shoot in spring shoot and summer shoot and autumn shoot (SSA-SP), while the C/N, ABA/GA, ZR/GA, IAA/GA of summer shoot in spring shoot and summer shoot (SS-SU) were higher than that of spring shoot in spring shoot and summer shoot (SS-SP), and the C/N, ABA/GA, ZR/GA, IAA/GA of autumn shoot in spring shoot and autumn shoot (SA-AU) were higher than that of spring shoot in spring shoot and autumn shoot (SA-SP). Before petal differentiation stage, GA content was high, ABA content, ZR content, ABA/GA and ZR/GA were all at low levels; after entering stamen differentiation stage and pistil differentiation stage, ABA content, ZR content, ABA/GA and ZR/GA all increased rapidly. Flower formation of the front end shoots was better, and the flower formation ability of middle and rear end shoots were weak, which was related to the difference in flower bud differentiation process, nutrient accumulation and endogenous hormone levels, and the hormones played a major regulatory role. The stamen differentiation stage (February 5 to February 20) was the critical period. Therefore, in the process of cultivation and management, special attention should be paid to the rational allocation of different types of female branches, the timing of topping and shoot release in summer and autumn, and trying to use hormones to regulate flower bud differentiation.

Key words: Hongmeiren, female branche, flower bud differentiation, endogenous hormone

CLC Number:

S666

WANG Zhihao, XI Xinyan, WANG Li, YANG Shuna, GAO Zhiyuan, YIN Yiming, ZOU Hui, JIA Huijuan. Flower bud formation and physiological biochemistry characteristics of Hongmeiren citrus hybrid in northern Zhejiang, China[J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1571-1581.

Figures/Tables 6

Fig.1 Sketch of sampling

Fig.2 Stages and characteristics of flower bud differentiation of Hongmeiren citrus hybrid a, Undifferentiated stage; b, Initial stage of flower buds; c, Sepal differentiation stage; d, Petal differentiation stage; e, Stamen differentiation stage; f, Pistil differentiation stage; GT, Growth tip; SE, Sepal primordial; PE, Petal primordial; SP, Stamen primordial; PP, Pistil primordial.

Fig.3 Comparison of flower bud differentiation from different female branch types of Hongmeiren A, SSA-SP (spring shoot in spring shoot and summer shoot and autumn shoot); B, SSA-SU (summer shoot in spring shoot and summer shoot and autumn shoot); C, SSA-AU (autumn shoot in spring shoot and summer shoot and autumn shoot); D, SS-SP (spring shoot in spring shoot and summer shoot); E, SS-SU (summer shoot in spring shoot and summer shoot); F, SA-SP (spring shoot in spring shoot and autumn shoot); G, SA-AU (autumn shoot in spring shoot and autumn shoot); H, S-SP (spring shoot in single shoot).

Fig.4 Comparison of hormone content in flower buds from different branches of Hongmeiren SSA-SP, Spring shoot in spring shoot and summer shoot and autumn shoot; SSA-SU, Summer shoot in spring shoot and summer shoot and autumn shoot; SSA-AU, Autumn shoot in spring shoot and summer shoot and autumn shoot; SS-SP, Spring shoot in spring shoot and summer shoot; SS-SU, Summer shoot in spring shoot and summer shoot; SA-SP, Spring shoot in spring shoot and autumn shoot; SA-AU, Autumn shoot in spring shoot and autumn shoot; S-SP, Spring shoot of one shoot. The same as below.

Fig.5 Comparison of hormone ratio in bud from different female branch types of Hongmeiren

Fig.6 Comparison of C/N in leaf from different female branch types of Hongmeiren

References 24

[1]	马静静, 俞忠, 陆慧, 等. 张家港市“红美人”柑橘设施栽培技术[J]. 上海农业科技, 2021(3): 62-63.
	MA J J, YU Z, LU H, et al. Facility cultivation techniques of ‘Hongmeiren’ citrus hybrid in Zhangjiagang[J]. Shanghai Agricultural Science and Technology, 2021(3): 62-63. (in Chinese)
[2]	柯甫志, 徐建国, 罗君琴, 等. 红美人柑橘果实套袋栽培试验[J]. 浙江农业科学, 2014, 55(6): 850-852.
	KE F Z, XU J G, LUO J Q, et al. Bagging cultivation experiment of ‘Hongmeiren’ citrus fruit[J]. Journal of Zhejiang Agricultural Sciences, 2014, 55(6): 850-852. (in Chinese)
[3]	赵通, 陈翠莲, 程丽, 等. ‘李光杏’花芽分化时期内源激素及碳氮比值的动态研究[J]. 干旱地区农业研究, 2020, 38(3): 97-104.
	ZHAO T, CHEN C L, CHENG L, et al. Dynamic study on endogenous hormones and C/N ratio during flower-bud differentiation of Li-Guang apricot[J]. Agricultural Research in the Arid Areas, 2020, 38(3): 97-104. (in Chinese with English abstract)
[4]	庞钰洁, 陶宁颖, 竺啸恒, 等. 根域限制栽培对桃花芽分化进程中碳氮比及ABA含量的影响[J]. 果树学报, 2018, 35(11): 1363-1373.
	PANG Y J, TAO N Y, ZHU X H, et al. Effect of root restriction on flower bud formation of FYuanmeng’ peach trees[J]. Journal of Fruit Science, 2018, 35(11): 1363-1373. (in Chinese with English abstract)
[5]	杨盛, 郝国伟, 张晓伟, 等. ‘玉露香梨’僵芽发生与内源激素和碳氮营养的关系[J]. 园艺学报, 2015, 42(6): 1057-1065.
	YANG S, HAO G W, ZHANG X W, et al. Effects of endogenous hormone, carbon and nitrogen nutrition on development of wizened bud in ‘Yulu Xiangli’ pear[J]. Acta Horticulturae Sinica, 2015, 42(6): 1057-1065. (in Chinese with English abstract)
[6]	林玲, 黄羽, 谢太理, 等. 3个葡萄品种花芽分化过程中内源激素含量变化初报[J]. 南方农业学报, 2012, 43(6): 806-809.
	LIN L, HUANG Y, XIE T L, et al. Changes of endogenous hormone content during flower bud differentiation in three grape varieties[J]. Journal of Southern Agriculture, 2012, 43(6): 806-809. (in Chinese with English abstract)
[7]	张琛, 刘辉, 郗笃隽, 等. 杭州和泰安甜樱桃不同发育阶段花芽内含物及花芽质量的比较研究[J]. 果树学报, 2021, 38(8): 1308-1318.
	ZHANG C, LIU H, XI D J, et al. Comparative study on flower bud inclusion and quality in different development stages of sweet cherry from Hangzhou and Tai’an[J]. Journal of Fruit Science, 2021, 38(8): 1308-1318. (in Chinese with English abstract)
[8]	吴志祥, 王令霞, 陶忠良, 等. 2个荔枝品种花芽分化期碳氮营养的变化[J]. 热带作物学报, 2006, 27(4): 25-28.
	WU Z X, WANG L X, TAO Z L, et al. Changes of carbonitride in two Litchi cultivars during flower bud differentiation[J]. Chinese Journal of Tropical Crops, 2006, 27(4): 25-28. (in Chinese with English abstract)
[9]	许伟东, 郑诚乐, 吴宪志, 等. 杨梅花芽生理分化期叶片碳氮含量动态变化[J]. 福建热作科技, 2009, 34(4): 18-20.
	XU W D, ZHENG C L /Y, WU X Z, et al. Dynamic changes of carbon and nitrogen contents in leaves of Myrica rubra during flower bud physiological differentiation[J]. Fujian Science & Technology of Tropical Crops, 2009, 34(4): 18-20. (in Chinese)
[10]	樊卫国, 刘国琴, 安华明, 等. 刺梨花芽分化期芽中内源激素和碳、氮营养的含量动态[J]. 果树学报, 2003, 20(1): 40-43.
	FAN W G, LIU G Q, AN H M, et al. Study on the changes of endogenous hormones, carbohydrate and nitrogen nutrition at the flower bud differentiation stage of Rosa roxburghii[J]. Journal of Fruit Science, 2003, 20(1): 40-43. (in Chinese with English abstract)
[11]	区善汉, 李雁群, 陈宜超, 等. 桂林福本和纽荷尔脐橙前期花芽形态分化比较研究[J]. 基因组学与应用生物学, 2009, 28(2): 316-320.
	OU S H, LI Y Q, CHEN Y C, et al. Comparative studies on morphological differentiation of flower bud in early stage between blessing navel orange and newhall orange in Guilin[J]. Genomics and Applied Biology, 2009, 28(2): 316-320. (in Chinese with English abstract)
[12]	区善汉, 李雁群, 麦适秋, 等. 3个新引进脐橙品种在桂林花芽分化的解剖学观察[J]. 分子植物育种, 2009, 7(4): 767-771.
	OU S H, LI Y Q, MAI S Q, et al. Morphological studies on flower bud differentiation of three navel orange varieties introduced newly in Guilin[J]. Molecular Plant Breeding, 2009, 7(4): 767-771. (in Chinese with English abstract)
[13]	区善汉, 李雁群, 麦适秋, 等. Lanelate脐橙花芽形态分化研究[J]. 西南农业学报, 2009, 22(6): 1670-1673.
	OU S H, LI Y Q, MAI S Q, et al. Morphological differentiation of flower bud of lanelate oranges[J]. Southwest China Journal of Agricultural Sciences, 2009, 22(6): 1670-1673. (in Chinese with English abstract)
[14]	麦适秋, 张敏, 莫健生, 等. 桂林Barnfield脐橙花芽分化形态观察[J]. 南方农业学报, 2012, 43(7): 1021-1024.
	MAI S Q, ZHANG M, MO J S, et al. Investigation on flower bud differentiation and morphology of Barnfield navel orange in Guilin[J]. Journal of Southern Agriculture, 2012, 43(7): 1021-1024. (in Chinese with English abstract)
[15]	区善汉, 李雁群, 梅正敏, 等. 迟熟蕉柑花芽分化观察研究[J]. 广西农业科学, 2009, 40(5): 548-551.
	OU S H, LI Y Q, MEI Z M, et al. Investigation of bud differentiation for late-ripening Jiaogan mandarins[J]. Guangxi Agricultural Sciences, 2009, 40(5): 548-551. (in Chinese with English abstract)
[16]	周翡云. 融安金柑和滑皮金柑花芽分化与结果习性研究[D]. 南宁: 广西大学, 2014.
	ZHOU F Y. Research on the flower development and fructification habit of Rong’ an kumquat and Huapi kumquat[D]. Nanning: Guangxi University, 2014. (in Chinese with English abstract)
[17]	王锦涛. 不同海拔对‘琯溪蜜柚’花芽分化的影响[D]. 福州: 福建农林大学, 2016.
	WANG J T. Effects of altitude on bud differentiation in Guanxi pomelo[D]. Fuzhou: Fujian Agriculture and Forestry University, 2016. (in Chinese with English abstract)
[18]	张丹, 李新国, 罗丽华, 等. 无籽蜜柚物候期与花芽分化形态学研究[J]. 福建农业学报, 2018, 33(1): 51-53.
	ZHANG D, LI X G, LUO L H, et al. Phenology and flower bud differentiation morphology of seedless honey pummelo[J]. Fujian Journal of Agricultural Sciences, 2018, 33(1): 51-53. (in Chinese with English abstract)
[19]	胡绍彬, 王锦涛, 赵秋月, 等. 不同海拔对‘琯溪蜜柚’春梢花芽分化的影响[J]. 热带作物学报, 2021, 42(4): 1029-1034.
	HU S B, WANG J T, ZHAO Q Y, et al. Influence of different altitude on flower bud differentiation of ‘guanximiyou’ pummel (Citrus grandis) in different altitudes[J]. Chinese Journal of Tropical Crops, 2021, 42(4): 1029-1034. (in Chinese with English abstract)
[20]	ZUO X Y, ZHANG D, WANG S X, et al. Expression of genes in the potential regulatory pathways controlling alternate bearing in ‘Fuji’ (Malus domestica Borkh.) apple trees during flower induction[J]. Plant Physiology and Biochemistry, 2018, 132: 579-589.
[21]	张上隆. PP₃₃₃与去叶处理对柑橘成花、内源激素含量及其平衡的影响[C]//张上隆. 纪念吴耕民教授诞生一百周年论文集. 北京: 中国农业科技出版社, 1995: 15-21.
[22]	黄迪辉, 黄辉白. 柑桔成花机理的研究I.与内源激素的关系[J]. 果树科学, 1992, 9(1): 13-18.
	HUANG D H, HUANG H B. Studies on the mechanism of citrus flower-bud formation Ⅰ. relations to endogenous hormones[J]. Journal of Fruit Science, 1992, 9(1): 13-18. (in Chinese with English abstract)
[23]	李欢, 李建兵, 黄家权. 外源多效唑对红肉蜜柚成花的影响[J]. 分子植物育种, 2019, 17(9): 3046-3052.
	LI H, LI J B, HUANG J Q. Effect of exogenous paclobutrazol on flowering of red pulp honey pomelo[J]. Molecular Plant Breeding, 2019, 17(9): 3046-3052. (in Chinese with English abstract)
[24]	付崇毅, 刘杰才, 崔世茂, 等. 低温对日光温室砂糖橘成花诱导及生理反应的影响[J]. 中国生态农业学报, 2013, 21(5): 572-579.
	FU C Y, LIU J C, CUI S M, et al. Flower induction and physiological response of Citrus reticulata “Shatangju” to low temperature under solar greenhouse condition[J]. Chinese Journal of Eco-Agriculture, 2013, 21(5): 572-579. (in Chinese with English abstract)

Flower bud formation and physiological biochemistry characteristics of Hongmeiren citrus hybrid in northern Zhejiang, China

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 24

Related Articles 8

Recommended Articles

Metrics

Comments

[1]	XU Hongxia, LI Xiaoying, GE Hang, ZHU Qixuan, CHEN Junwei. Transcriptome-based analysis of the role of endogenous hormones in regulating flower development in loquat (Eriobotrya japonica Lindl.) [J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1648-1661.
[2]	NIU Erli, PEI Hongbin, DING Jian, ZHU Shenlong. Flower bud differentiation and fruit development of three olive cultivars [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 887-896.
[3]	WEN Mingxia, ZHANG Shunchang, WU Shaohui, HU Lipeng, WANG Peng, HUANG Bei. Effect of protein hydrolysate-based amino-acid fertilizers on fruit quality of Hongmeiren citrus hybrid [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 422-428.
[4]	WANG Chengkuan, HUANG Zhendong, LIU Xingquan, HONG Xiaoling. Effects of meteorological factors on fruit quality of Hongmeiren citrus [J]. , 2020, 32(10): 1798-1808.
[5]	GUO Jing 1， ZHOU Kejin2，*. The differences of endogenous hormones level and their response to ripener among the different rapeseed varieties [J]. , 2016, 28(3): 383-.
[6]	FAN Xiao\\|xue1， SONG Bo1， XU Hai1， CHEN Long\\|zheng1，*， XU Zhi\\|gang2， YUAN Xi\\|han1. Effects of light\\|emitting diodes on contents of endogenous hormones in non\\|heading Chinese cabbage and tomato [J]. , 2015, 27(11): 1927-.
[7]	YUAN Yan\\|bo;WANG Li\\|hui;YU Xiao\\|nan*. Comparative analysis of endogenous hormones during dormant bud development of Chinese herbaceous peony [J]. , 2014, 26(1): 0-60.
[8]	YU Min;WANG Shao-min;ZHOU Hong;ZHANG Hai-zhen;FU Xiao-lu. Effects of plant regulators NAA and 6-BA on bulb growth and endogenous hormone content of Lycoris haywardii [J]. , 2013, 25(4): 0-771.