Effect of glycine betaine on physiological characteristics of eggplant seedlings under high temperature stress

doi:10.3969/j.issn.1004-1524.20221258

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (9): 2097-2108.DOI: 10.3969/j.issn.1004-1524.20221258

• Horticultural Science • Previous Articles Next Articles

Effect of glycine betaine on physiological characteristics of eggplant seedlings under high temperature stress

GAO Xiaoping(), ZHANG Jing, NIU Tianhang, LIU Yang, CHANG Youlin, LIU Sitian, XIE Jianming()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2022-08-29 Online:2023-09-25 Published:2023-10-09

Abstract

Abstract:

To investigate the mitigating effect of exogenous glycine betaine on the injury of eggplant under high temperature stress, eggplant variety ‘Bride’ was used as the test material. Seedlings were foliar sprayed with 0 (CK), 20 (T1), 40 (T2) and 60 mmol·L^-1 (T3) glycine betaine at four leaves and one heart stage for 3 d. The changes in biomass accumulation, photosynthetic characteristics, Calvin cycle key enzymes activity and antioxidant enzymes activity were measured in a climatic chamber at 40 ℃ after high temperature stress for 48 h. The results showed that the growth of eggplant seedlings under high temperature stress could be alleviated to different degrees by spraying different concentrations of glycine betaine, with the most obvious effect of 40 mmol·L^-1 treatment. Compared with CK, the height, fresh weight, root length, root surface area, root volume and root tip number of T2 treated seedlings were significantly (P<0.05) increased by 32.97%, 34.17%, 19.47%, 16.55%, 32.20% and 25.29%, respectively; T2 treatment significantly increased chlorophyll a, chlorophyll b and total chlorophyll content; meanwhile, it significantly increased leaf photosynthetic parameters net photosynthesis rate (P_n), stomatal conductance (G_s) and transpiration rate (T_r) by 51.26%, 40.24% and 49.86%, respectively, and significantly increased the activities of Calvin cycle related enzymes activity such as fructose 1,6 diphosphatase (FBPase), fructose 1,6-bisphosphate aldolase (FBA), 3-phosphoglyceraldehyde dehydrogenase (GAPDH) and transketolase (TK), and the actual photochemical efficiency of photosystem Ⅱ. T2 treatment significantly increased leaf superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity and proline (Pro) content by 35.62%, 109.65%, 72.99% and 58.13%, respectively. Glycine betaine at 40 mmol·L^-1 reduced the degradation of chlorophyll accumulation, maintained photosynthetic carbon cycle enzyme activity, promoted photosynthesis, improved leaf antioxidant enzymes activity, and reduced cell membrane injury, thus promoting the growth of eggplant seedlings under high temperature stress

Key words: glycine betaine, eggplant, high temperature stress, physiological characteristic

CLC Number:

S626.5

GAO Xiaoping, ZHANG Jing, NIU Tianhang, LIU Yang, CHANG Youlin, LIU Sitian, XIE Jianming. Effect of glycine betaine on physiological characteristics of eggplant seedlings under high temperature stress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2097-2108.

Figures/Tables 12

References 46

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处理 Treatment	株高 Plant height/cm	全株鲜重 Plant fresh weight/g	全株干重 Plant dry weight/g	地上部鲜重 Shoot fresh weight/g	地上部干重 Shoot dry weight/g
CK	9.10±0.12 c	4.80±0.23 c	0.81±0.01 c	4.64±0.07 b	0.71±0.01 b
T1	10.10±0.17 b	5.39±0.13 b	0.83±0.01 bc	4.89±0.12 b	0.73±0.01 b
T2	12.10±0.12 a	6.44±0.18 a	0.90±0.01 a	5.74±0.24 a	0.82±0.02 a
T3	12.07±0.09 a	6.04±0.04 a	0.88±0.03 ab	5.59±0.07 a	0.81±0.01 a

处理 Treatment	株高 Plant height/cm	全株鲜重 Plant fresh weight/g	全株干重 Plant dry weight/g	地上部鲜重 Shoot fresh weight/g	地上部干重 Shoot dry weight/g
CK	9.10±0.12 c	4.80±0.23 c	0.81±0.01 c	4.64±0.07 b	0.71±0.01 b
T1	10.10±0.17 b	5.39±0.13 b	0.83±0.01 bc	4.89±0.12 b	0.73±0.01 b
T2	12.10±0.12 a	6.44±0.18 a	0.90±0.01 a	5.74±0.24 a	0.82±0.02 a
T3	12.07±0.09 a	6.04±0.04 a	0.88±0.03 ab	5.59±0.07 a	0.81±0.01 a

处理 Treatment	根长 Root length/cm	根表面积 Surface area/cm²	根体积 Root volume/cm³	根尖数 Root tip
CK	985.28±6.68 c	150.90±3.79 b	1.77±0.04 c	2 214.67±101.18 c
T1	1 048.91±22.61 b	159.57±4.26 ab	2.09±0.13 ab	2 375.67±100.22 bc
T2	1 177.07±25.88 a	175.88±6.99 a	2.34±0.04 a	2 774.67±78.77 a
T3	1 016.42±7.69 bc	164.43±5.12 ab	1.85±0.11 bc	2 544.67±46.19 ab

处理 Treatment	根长 Root length/cm	根表面积 Surface area/cm²	根体积 Root volume/cm³	根尖数 Root tip
CK	985.28±6.68 c	150.90±3.79 b	1.77±0.04 c	2 214.67±101.18 c
T1	1 048.91±22.61 b	159.57±4.26 ab	2.09±0.13 ab	2 375.67±100.22 bc
T2	1 177.07±25.88 a	175.88±6.99 a	2.34±0.04 a	2 774.67±78.77 a
T3	1 016.42±7.69 bc	164.43±5.12 ab	1.85±0.11 bc	2 544.67±46.19 ab

处理 Treatment	叶绿素a含量 Chlorophyll a content/(mg·g^-1)	叶绿素b含量 Chlorophyll b content/(mg·g^-1)	总叶绿素含量 Total chlorophyll content/(mg·g^-1)	叶绿素a/叶绿素b Chla/Chlb
CK	1.90±0.04 c	0.75±0.03 b	2.65±0.04 c	2.54±0.13 b
T1	2.22±0.06 b	0.74±0.03 b	2.96±0.03 b	3.03±0.21 a
T2	2.57±0.04 a	0.84±0.02 a	3.41±0.02 a	3.07±0.11 a
T3	2.17±0.04 b	0.81±0.01 b	2.98±0.05 b	2.68±0.05 ab

Effect of glycine betaine on physiological characteristics of eggplant seedlings under high temperature stress

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处理 Treatment	净光合速率 P_n/(μmol·m^-2·s^-1)	气孔导度 G_s/(mmol·m^-2·s^-1)	蒸腾速率 T_r/(mmol·m^-2·s^-1)	胞间CO₂浓度 C_i/(μmol·mol^-1)
CK	5.93±0.30 c	244.33±4.98 d	3.47±0.09 d	403.67±7.84 a
T1	6.33±0.42 c	306.67±2.85 c	4.30±0.12 c	385.33±2.33 b
T2	8.97±0.35 a	342.67±4.98 a	5.20±0.06 a	344.33±5.04 c
T3	7.77±0.24 b	325.67±2.03 b	4.83±0.09 b	390.67±3.28 ab

处理 Treatment	最大光化学效率 F_v/F_m	实际光化学效率 ΦPSⅡ	光化学猝灭系数 qP	非光化学猝灭系数 NPQ
CK	0.683±0.003 c	0.531±0.013 c	0.839±0.010 c	0.178±0.003 a
T1	0.756±0.004 b	0.554±0.009 bc	0.878±0.013 b	0.166±0.004 ab
T2	0.797±0.003 a	0.601±0.006 a	0.899±0.011 ab	0.150±0.004 c
T3	0.744±0.004 b	0.567±0.009 b	0.916±0.006 a	0.156±0.004 bc

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