Preparation and efficacy evaluation of lotus root relieving alcoholism functional gel fudge

doi:10.3969/j.issn.1004-1524.20230406

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (2): 404-415.DOI: 10.3969/j.issn.1004-1524.20230406

• Food Science • Previous Articles Next Articles

Preparation and efficacy evaluation of lotus root relieving alcoholism functional gel fudge

YANG Zihan¹^,²(), WU Weijie², GAO Yuan², LIU Ruiling², SHENTU Xuping¹, GAO Haiyan², CHEN Hangjun²^,^*()

1. School of Life Sciences, China Jiliang University, Hangzhou 310018, China
2. Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Postharvest Preservation and Processing of Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-03-29 Online:2024-02-25 Published:2024-03-05

Abstract

Abstract:

This paper focuses on the extraction process conditions of the antidote components of lotus roots and the antidote efficacy of the fudge prepared. The extraction solvents, solid-liquid ratio, extraction time and extraction temperature were investigated by single-factor and orthogonal experiments using lotus root as the raw material and the in vitro activation rate of alcohol dehydrogenase (ADH) as the index. The composition of the lotus root extract was analyzed and used as the raw material for the preparation of alcoholic functional gummies. The scavenging rates of DPPH radicals, ABTS⁺radicals and ·OH radicals were determined by adding different concentrations of lotus root extracts to the gel fudge. The results showed that the best extraction process of lotus root extract was 100% methanol, 1∶40 ratio, 60 ℃ and 5 h. The in vitro activation rate of ADH was (19.60±2.10)%, and 433 components were analyzed by UPLC-QTOF-MS/MS, the main substances are phenolic acids and flavonoids. The IC₅₀ of lotus root gummy extract for the scavenging of ABTS⁺radical, ·OH radical and DPPH radical were 14.59 mg·mL^-1, 101.96 mg·mL^-1 and 5.54 mg·mL^-1, respectively. Animal experiments demonstrated that lotus root relieving alcoholism functional gel fudge significantly increased (P<0.05) and decreased (P<0.05) the liver ADH activity in mice after alcohol consumption. It also prolonged the mean latency time of intoxication and shortened the recovery time of the reflex after intoxication. The results of this study suggest that lotus root relieving alcoholism functional gel fudge have certain antidrinking effects. The results of this study provide a new theoretical and practical basis for the development of functional food with the effect of alcohol detoxification and liver protection.

Key words: lotus root, relieving alcoholism, gel fudge, process optimization

CLC Number:

TS201.1

YANG Zihan, WU Weijie, GAO Yuan, LIU Ruiling, SHENTU Xuping, GAO Haiyan, CHEN Hangjun. Preparation and efficacy evaluation of lotus root relieving alcoholism functional gel fudge[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 404-415.

Figures/Tables 12

Fig.1 Effect of different volume fractions of methanol as solvent on the activation rate of ethanol dehydrogenase from lotus root extract The data without the same lowecase letters show the significant difference (P<0.05). The same as below.

Fig.2 Effect of different extraction temperatures on the activation rate of ethanol dehydrogenase from lotus root extracts

Fig.3 Effect of different extraction times on the activation rate of ethanol dehydrogenase from lotus root extracts

Fig.4 Effect of different material-liquid ratios on the activation rate of ethanol dehydrogenase from lotus root extract

Table 1 Orthogonal experimental factors and level design

水平 Level	A甲醇体积分数 Methanol concentration/%	B提取时间 Extraction time/h	C提取温度 Extraction temperature/℃	D料液比 Solid-liquid ration(m/V)
1	80	3	50	1∶40
2	90	4	60	1∶45
3	100	5	70	1∶50

Table 2 Results of orthogonal experiments

试验编号 Test No.	因素 Factor				ADH激活率 ADH activation rate/%
试验编号 Test No.	A	B	C	D	ADH激活率 ADH activation rate/%
1	1	1	1	1	5.30
2	1	2	2	2	7.80
3	1	3	3	3	7.60
4	2	1	2	3	13.96
5	2	2	3	1	11.35
6	2	3	1	2	9.81
7	3	1	3	2	18.31
8	3	2	1	3	19.17
9	3	3	2	1	19.60
k₁	6.9	12.523	11.427	12.083	—
k₂	11.707	12.773	13.787	11.973	—
k₃	19.027	12.337	12.420	13.577	—
R	12.127	0.436	2.360	1.604	—

Table 3 Analysis of variance for the extraction process of antidote components from lotus root

因素 Factor	偏差平方和 Square of deviance	自由度 Degree of freedom	F比 F-ratio	F临界值 F marginal value	显著性 Significance
A	223.742	2	776.882	19	*
B	0.288	2	1	19
C	8.424	2	29.25	19	*
D	4.813	2	16.712	19
误差Error	0.29	2

Fig.5 Lotus root extract MRM multi-peak figure A, Positive ion mode; B, Negative ion mode.

Fig.6 lotus root extract metabolites distribution

Fig.7 Effects of different concentrations of lotus root extract on ABTS+ free radicals(A), ·OH radical (B) and DPPH free radicals (C) clearance rate

Table 4 Evaluation of the effect of lotus root relieving alcoholism funcitional gel fudge

分组 Group	实验数量/只 Experimental quantity	醉酒潜伏时间 The latency time/min	翻正反射恢复时间 The recovery time of the positive reflex/min	醉酒率 Drunk rate/%
模型组Model group	10	5.29±1.2	150.76±9.31	100
莲藕提取液干预组Lotus root extract intervention group	10	20.58±6.24^*	53.89±19.51^**	80

Fig.8 Lotus root relieving alcoholism functional gel fudge on ethanol content in mouse serum and ADH activity in mouse liver

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Preparation and efficacy evaluation of lotus root relieving alcoholism functional gel fudge

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