Extraction, structural characterization and antioxidant activity of polysaccharide from Dendrobium officinale in Yandang Mountain, China

doi:10.3969/j.issn.1004-1524.20230543

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (8): 1898-1908.DOI: 10.3969/j.issn.1004-1524.20230543

• Food Science • Previous Articles Next Articles

Extraction, structural characterization and antioxidant activity of polysaccharide from Dendrobium officinale in Yandang Mountain, China

ZHAO Xiaoliang¹(), LU Yun¹, KANG Xingxing¹, LONG Zeyu¹, ZHENG Xiaojie²^,^*()

1. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
2. Institute of Food Science, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, Zhejiang, China

Received:2023-04-27 Online:2024-08-25 Published:2024-09-06
Contact: ZHENG Xiaojie

Abstract

Abstract:

In the present study, the fresh stem of Dendrobium officinale in Yandang Mountain was selected as the test object, the liquid-to-material ratio, extraction temperature, extration time and extraction times were selected as the key factors, as well as the yield of polysaccharide being response value, to optime the extraction process of polysaccharide from D. officinale in Yandang Mountain. The structure of the extracted polyssacharide was preliminarily explored by Fourier infrared spectroscopy and high performance liquid chromatography (HPLC). Besides, the in vitro activity of polysaccharides to scavenge free radicals was examined. The results showed that the optimal process conditions for the extraction of polysaccharide were liquid-to-material ratio of 55∶1 (mL·g^-1), extraction temperature of 80 ℃, extraction time of 130 min, and extraction times of 2 times. The actual polysaccharide yield under these conditions was 31.02%. By infrared spectroscopic analysis, it was shown that the polysaccharide contained both α-type and β-type glycosidic bonds, and the extracted polysaccharide was composed of mannose, glucuronic acid, galacturonic acid, glucose and xylose, and the amount of substance ratio of the above monosaccharides was 1.57∶0.49∶0.99∶2.08∶1.00. In vitro, the extracted polysaccharide could scavenge 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radicals, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals, hydroxyl free radicals and superoxide anion, and the determined half maximal inhibitory concentrations (IC₅₀) were 2.46, 4.47, 5.60, 2.66 mg·mL^-1, respectively, indicating that the extracted polysaccharide had antioxidant activity.

Key words: Dendrobium officinale, polysaccharide, process optimization, structural characterization, antioxidant activity

CLC Number:

TQ281
S567

ZHAO Xiaoliang, LU Yun, KANG Xingxing, LONG Zeyu, ZHENG Xiaojie. Extraction, structural characterization and antioxidant activity of polysaccharide from Dendrobium officinale in Yandang Mountain, China[J]. Acta Agriculturae Zhejiangensis, 2024, 36(8): 1898-1908.

Figures/Tables 12

Table 1 Factors and levels in response surface analysis

编码水平 Coding level	各因素的设定值Setting values for factors
编码水平 Coding level	LTM/(mL·g^-1)	ET/min	ETP/℃	ETS
-1	30	60	50	1
0	45	120	70	2
1	60	180	90	3

Fig.1 Effect of liquid-to-material ratio, extraction time, extraction temperature and extraction times on the yield of polysaccharides from Dendrobium of ficinale

Table 2 Design and results of response surface experiment

编号 No.	各因素的编码水平Coding levels of factor				Y/%
编号 No.	X₁	X₂	X₃	X₄	Y/%
1	-1	0	0	1	28.21
2	0	-1	0	1	26.82
3	0	1	-1	0	25.31
4	0	0	-1	-1	24.63
5	0	1	1	0	28.26
6	-1	0	1	0	27.86
7	-1	0	-1	0	25.32
8	0	0	-1	1	25.15
9	0	-1	1	0	28.56
10	0	0	1	1	29.92
11	0	0	0	0	31.05
12	0	-1	0	-1	26.96
13	-1	-1	0	0	26.13
14	1	1	0	0	27.29
15	0	0	0	0	30.76
16	0	0	0	0	31.02
17	0	1	0	1	26.86
18	1	0	1	0	31.13
19	0	0	0	0	30.45
20	0	0	1	-1	29.80
21	0	-1	-1	0	24.09
22	0	0	0	0	30.86
23	0	1	0	-1	26.96
24	1	-1	0	0	27.21
25	1	0	-1	0	24.65
26	1	0	0	-1	28.16
27	-1	0	0	-1	26.36
28	-1	1	0	0	27.15
29	1	0	0	1	27.86

Table 3 Analysis of variance of regression model

来源 Source	平方和 Sum of squares	均方 Mean square	F值 F value	P值 P value
模型Model	127.03	9.07	66.30	<0.000 1
X₁	2.31	2.31	16.91	0.001 1
X₂	0.35	0.35	2.58	0.130 3
X₃	57.99	57.99	423.77	<0.000 1
X₄	0.32	0.32	2.32	0.150 4
X₁X₂	0.22	0.22	1.61	0.224 6
X₁X₃	3.88	3.88	28.36	0.000 1
X₁X₄	1.16	1.16	8.44	0.011 5
X₂X₃	0.58	0.58	4.22	0.059 1
X₂X₄	0.01	0.01	0.0029	0.957 6
X₃X₄	0.04	0.04	0.2923	0.597 3
$X 12$	16.78	16.78	122.58	<0.000 1
$X 22$	35.05	35.05	256.09	<0.000 1
$X 32$	24.40	24.40	178.28	<0.000 1
$X 42$	15.85	15.85	115.82	<0.000 1
残差Residual	1.92	0.14	—	—
失拟项Lack of Fit	1.68	0.17	2.87	0.161 0
纯误差Pure error	0.23	0.06	—	—

Table 3 Analysis of variance of regression model

来源 Source	平方和 Sum of squares	均方 Mean square	F值 F value	P值 P value
模型Model	127.03	9.07	66.30	<0.000 1
X₁	2.31	2.31	16.91	0.001 1
X₂	0.35	0.35	2.58	0.130 3
X₃	57.99	57.99	423.77	<0.000 1
X₄	0.32	0.32	2.32	0.150 4
X₁X₂	0.22	0.22	1.61	0.224 6
X₁X₃	3.88	3.88	28.36	0.000 1
X₁X₄	1.16	1.16	8.44	0.011 5
X₂X₃	0.58	0.58	4.22	0.059 1
X₂X₄	0.01	0.01	0.0029	0.957 6
X₃X₄	0.04	0.04	0.2923	0.597 3
$X 12$	16.78	16.78	122.58	<0.000 1
$X 22$	35.05	35.05	256.09	<0.000 1
$X 32$	24.40	24.40	178.28	<0.000 1
$X 42$	15.85	15.85	115.82	<0.000 1
残差Residual	1.92	0.14	—	—
失拟项Lack of Fit	1.68	0.17	2.87	0.161 0
纯误差Pure error	0.23	0.06	—	—

Fig.2 Response surface and contour plots of the interaction of various factors to the polysaccharide yield of Dendrobium officinale

Fig.3 Ultraviolet spectrum of polysaccharide extracted from Dendrobium officinale

Fig.4 Infrared spectroscopy of polysaccharide from Dendrobium officinale

Fig.5 Liquid chromatograms of the mixed standard solution of monosaccharides and the polysaccharide hydrolysate of Dendrobium officinale 1-9 represent mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, fucose, respectively.

Fig.6 Scavenging effects of polysaccharide from Dendrobium officinale (PDO) and vitamin C on DPPH free radicals

Fig.7 Scavenging effects of polysaccharide from Dendrobium officinale (PDO) and vitamin C on ABTS free radicals

Fig.8 Scavenging effects of polysaccharide from Dendrobium officinale (PDO) and vitamin C on hydroxyl free radicals

Fig.9 Scavenging effects of polysaccharide from Dendrobium officinale (PDO) and vitamin C on superoxide anion

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Extraction, structural characterization and antioxidant activity of polysaccharide from Dendrobium officinale in Yandang Mountain, China

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